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. 1997 Sep;63(9):3698–3702. doi: 10.1128/aem.63.9.3698-3702.1997

Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures.

L K Poulsen 1, H M Dalton 1, M L Angles 1, K C Marshall 1, S Molin 1, A E Goodman 1
PMCID: PMC168676  PMID: 9293021

Abstract

A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells; a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.

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Selected References

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