Abstract
Two naphthalene-degrading bacteria, Pseudomonas putida G7 and Pseudomonas sp. strain NCIB 9816-4, were chemotactically attracted to naphthalene in drop assays and modified capillary assays. Growth on naphthalene or salicylate induced the chemotactic response. P. putida G7 was also chemotactic to biphenyl; other polyaromatic hydrocarbons that were tested did not appear to be chemoattractants for either Pseudomonas strain. Strains that were cured of the naphthalene degradation plasmid were not attracted to naphthalene.
Full Text
The Full Text of this article is available as a PDF (2.3 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973 Jan;74(1):77–91. doi: 10.1099/00221287-74-1-77. [DOI] [PubMed] [Google Scholar]
- Barnsley E. A. The induction of the enzymes of naphthalene metabolism in pseudomonads by salicylate and 2-aminobenzoate. J Gen Microbiol. 1975 May;88(1):193–196. doi: 10.1099/00221287-88-1-193. [DOI] [PubMed] [Google Scholar]
- Bateman J. N., Speer B., Feduik L., Hartline R. A. Naphthalene association and uptake in Pseudomonas putida. J Bacteriol. 1986 Apr;166(1):155–161. doi: 10.1128/jb.166.1.155-161.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunn N. W., Gunsalus I. C. Transmissible plasmid coding early enzymes of naphthalene oxidation in Pseudomonas putida. J Bacteriol. 1973 Jun;114(3):974–979. doi: 10.1128/jb.114.3.974-979.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eaton R. W. Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid. J Bacteriol. 1994 Dec;176(24):7757–7762. doi: 10.1128/jb.176.24.7757-7762.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fahrner K. A., Block S. M., Krishnaswamy S., Parkinson J. S., Berg H. C. A mutant hook-associated protein (HAP3) facilitates torsionally induced transformations of the flagellar filament of Escherichia coli. J Mol Biol. 1994 Apr 29;238(2):173–186. doi: 10.1006/jmbi.1994.1279. [DOI] [PubMed] [Google Scholar]
- Friedman A. M., Long S. R., Brown S. E., Buikema W. J., Ausubel F. M. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene. 1982 Jun;18(3):289–296. doi: 10.1016/0378-1119(82)90167-6. [DOI] [PubMed] [Google Scholar]
- Ghosal D., You I. S., Gunsalus I. C. Nucleotide sequence and expression of gene nahH of plasmid NAH7 and homology with gene xylE of TOL pWWO. Gene. 1987;55(1):19–28. doi: 10.1016/0378-1119(87)90244-7. [DOI] [PubMed] [Google Scholar]
- Harwood C. S., Nichols N. N., Kim M. K., Ditty J. L., Parales R. E. Identification of the pcaRKF gene cluster from Pseudomonas putida: involvement in chemotaxis, biodegradation, and transport of 4-hydroxybenzoate. J Bacteriol. 1994 Nov;176(21):6479–6488. doi: 10.1128/jb.176.21.6479-6488.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harwood C. S., Parales R. E. The beta-ketoadipate pathway and the biology of self-identity. Annu Rev Microbiol. 1996;50:553–590. doi: 10.1146/annurev.micro.50.1.553. [DOI] [PubMed] [Google Scholar]
- Harwood C. S., Rivelli M., Ornston L. N. Aromatic acids are chemoattractants for Pseudomonas putida. J Bacteriol. 1984 Nov;160(2):622–628. doi: 10.1128/jb.160.2.622-628.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Masduki A., Nakamura J., Ohga T., Umezaki R., Kato J., Ohtake H. Isolation and characterization of chemotaxis mutants and genes of Pseudomonas aeruginosa. J Bacteriol. 1995 Feb;177(4):948–952. doi: 10.1128/jb.177.4.948-952.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Platt A., Shingler V., Taylor S. C., Williams P. A. The 4-hydroxy-2-oxovalerate aldolase and acetaldehyde dehydrogenase (acylating) encoded by the nahM and nahO genes of the naphthalene catabolic plasmid pWW60-22 provide further evidence of conservation of meta-cleavage pathway gene sequences. Microbiology. 1995 Sep;141(Pt 9):2223–2233. doi: 10.1099/13500872-141-9-2223. [DOI] [PubMed] [Google Scholar]
- Serdar C. M., Gibson D. T. Isolation and characterization of altered plasmids in mutant strains of Pseudomonas putida NCIB 9816. Biochem Biophys Res Commun. 1989 Oct 31;164(2):764–771. doi: 10.1016/0006-291x(89)91525-8. [DOI] [PubMed] [Google Scholar]
- Serdar C. M., Gibson D. T. Studies of nucleotide sequence homology between naphthalene-utilizing strains of bacteria. Biochem Biophys Res Commun. 1989 Oct 31;164(2):772–779. doi: 10.1016/0006-291x(89)91526-x. [DOI] [PubMed] [Google Scholar]
- Simon M. J., Osslund T. D., Saunders R., Ensley B. D., Suggs S., Harcourt A., Suen W. C., Cruden D. L., Gibson D. T., Zylstra G. J. Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4. Gene. 1993 May 15;127(1):31–37. doi: 10.1016/0378-1119(93)90613-8. [DOI] [PubMed] [Google Scholar]
- Yen K. M., Gunsalus I. C. Plasmid gene organization: naphthalene/salicylate oxidation. Proc Natl Acad Sci U S A. 1982 Feb;79(3):874–878. doi: 10.1073/pnas.79.3.874. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yen K. M., Serdar C. M. Genetics of naphthalene catabolism in pseudomonads. Crit Rev Microbiol. 1988;15(3):247–268. doi: 10.3109/10408418809104459. [DOI] [PubMed] [Google Scholar]
- You I. S., Ghosal D., Gunsalus I. C. Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate hydroxylase gene (nahG) and its 3'-flanking region. Biochemistry. 1991 Feb 12;30(6):1635–1641. doi: 10.1021/bi00220a028. [DOI] [PubMed] [Google Scholar]
- You I. S., Ghosal D., Gunsalus I. C. Nucleotide sequence of plasmid NAH7 gene nahR and DNA binding of the nahR product. J Bacteriol. 1988 Dec;170(12):5409–5415. doi: 10.1128/jb.170.12.5409-5415.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van der Meer J. R. Evolution of novel metabolic pathways for the degradation of chloroaromatic compounds. Antonie Van Leeuwenhoek. 1997 Feb;71(1-2):159–178. doi: 10.1023/a:1000166400935. [DOI] [PubMed] [Google Scholar]