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. 1997 Jan 22;264(1378):45–51. doi: 10.1098/rspb.1997.0007

Kinematic analysis of shear displacement as a means for operating mechanotransduction channels in the contact region between adjacent stereocilia of mammalian cochlear hair cells.

D N Furness 1, D E Zetes 1, C M Hackney 1, C R Steele 1
PMCID: PMC1688222  PMID: 9061959

Abstract

In sensory hair cells of the cochlea, deflection of the stereociliary bundle results in direct mechanical gating of mechanoelectrical transduction channels, a function generally attributed to the tip link running between the tips of short stereocilia and the sides of adjacent taller ones. However, immunocytochemical experiments indicate that the channels may not be associated with the tip link but occur just below it in a region of contact between the stereocilia. To determine whether transduction channels in this location could be operated during physiologically appropriate deflections as effectively by shear displacement as if they were associated with the tip link, a two dimensional kinematic analysis of relative motion between stereocilia has been performed assuming contact between stereocilia is maintained during deflection. Bundle geometry and dimensions were determined from transmission electron micrographs of hair cells from several frequency locations between 0.27 and 13.00 kHz in the guinea-pig cochlea. The analysis indicates that for a 10 nm deflection of the tallest stereocilia of both inner and outer hair cells, i.e. within the range of the maximum sensitivity of mammalian hair bundles, the average shear displacement in the contact region would be 1.6 nm, but that it increases systematically towards higher frequency regions for outer hair cells. This displacement is comparable in magnitude to tip-link elongation for individual stereociliary pairs.

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Selected References

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  1. Corey D. P., Hudspeth A. J. Ionic basis of the receptor potential in a vertebrate hair cell. Nature. 1979 Oct 25;281(5733):675–677. doi: 10.1038/281675a0. [DOI] [PubMed] [Google Scholar]
  2. Corey D. P., Hudspeth A. J. Response latency of vertebrate hair cells. Biophys J. 1979 Jun;26(3):499–506. doi: 10.1016/S0006-3495(79)85267-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crawford A. C., Evans M. G., Fettiplace R. Activation and adaptation of transducer currents in turtle hair cells. J Physiol. 1989 Dec;419:405–434. doi: 10.1113/jphysiol.1989.sp017878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Flock A., Flock B., Murray E. Studies on the sensory hairs of receptor cells in the inner ear. Acta Otolaryngol. 1977 Jan-Feb;83(1-2):85–91. doi: 10.3109/00016487709128817. [DOI] [PubMed] [Google Scholar]
  5. Furness D. N., Hackney C. M. Cross-links between stereocilia in the guinea pig cochlea. Hear Res. 1985 May;18(2):177–188. doi: 10.1016/0378-5955(85)90010-3. [DOI] [PubMed] [Google Scholar]
  6. Geisler C. D. A model of stereociliary tip-link stretches. Hear Res. 1993 Feb;65(1-2):79–82. doi: 10.1016/0378-5955(93)90203-d. [DOI] [PubMed] [Google Scholar]
  7. Greenwood D. D. A cochlear frequency-position function for several species--29 years later. J Acoust Soc Am. 1990 Jun;87(6):2592–2605. doi: 10.1121/1.399052. [DOI] [PubMed] [Google Scholar]
  8. Hackney C. M., Furness D. N., Benos D. J., Woodley J. F., Barratt J. Putative immunolocalization of the mechanoelectrical transduction channels in mammalian cochlear hair cells. Proc Biol Sci. 1992 Jun 22;248(1323):215–221. doi: 10.1098/rspb.1992.0064. [DOI] [PubMed] [Google Scholar]
  9. Howard J., Roberts W. M., Hudspeth A. J. Mechanoelectrical transduction by hair cells. Annu Rev Biophys Biophys Chem. 1988;17:99–124. doi: 10.1146/annurev.bb.17.060188.000531. [DOI] [PubMed] [Google Scholar]
  10. Jacobs R. A., Hudspeth A. J. Ultrastructural correlates of mechanoelectrical transduction in hair cells of the bullfrog's internal ear. Cold Spring Harb Symp Quant Biol. 1990;55:547–561. doi: 10.1101/sqb.1990.055.01.053. [DOI] [PubMed] [Google Scholar]
  11. Jiang D., Furness D. N., Hackney C. M., Lopez D. E. Microslicing of the resin-embedded cochlea in comparison with the surface preparation technique for analysis of hair cell number and morphology. Br J Audiol. 1993 Jun;27(3):195–203. doi: 10.3109/03005369309076693. [DOI] [PubMed] [Google Scholar]
  12. Lim D. J. Functional structure of the organ of Corti: a review. Hear Res. 1986;22:117–146. doi: 10.1016/0378-5955(86)90089-4. [DOI] [PubMed] [Google Scholar]
  13. Osborne M. P., Comis S. D., Pickles J. O. Morphology and cross-linkage of stereocilia in the guinea-pig labyrinth examined without the use of osmium as a fixative. Cell Tissue Res. 1984;237(1):43–48. doi: 10.1007/BF00229198. [DOI] [PubMed] [Google Scholar]
  14. Pickles J. O. A model for the mechanics of the stereociliar bundle on acousticolateral hair cells. Hear Res. 1993 Aug;68(2):159–172. doi: 10.1016/0378-5955(93)90120-p. [DOI] [PubMed] [Google Scholar]

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