Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1997 Dec;63(12):4965–4968. doi: 10.1128/aem.63.12.4965-4968.1997

Conditions for natural transformation of Ralstonia solanacearum.

F Bertolla 1, F Van Gijsegem 1, X Nesme 1, P Simonet 1
PMCID: PMC168825  PMID: 9406418

Abstract

The development of competence allowing natural transformation of Ralstonia solanacearum was found to occur during exponential growth and not in response to any excreted factors. Linear DNAs were effectively integrated by recombination requiring a minimum of 50 bp of homologous DNA. Therefore, DNA from other genera and species were ineffective.

Full Text

The Full Text of this article is available as a PDF (165.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arlat M., Van Gijsegem F., Huet J. C., Pernollet J. C., Boucher C. A. PopA1, a protein which induces a hypersensitivity-like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum. EMBO J. 1994 Feb 1;13(3):543–553. doi: 10.1002/j.1460-2075.1994.tb06292.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ballard R. W., Palleroni N. J., Doudoroff M., Stanier R. Y., Mandel M. Taxonomy of the aerobic pseudomonads: Pseudomonas cepacia, P. marginata, P. alliicola and P. caryophylli. J Gen Microbiol. 1970 Feb;60(2):199–214. doi: 10.1099/00221287-60-2-199. [DOI] [PubMed] [Google Scholar]
  3. Carlson C. A., Pierson L. S., Rosen J. J., Ingraham J. L. Pseudomonas stutzeri and related species undergo natural transformation. J Bacteriol. 1983 Jan;153(1):93–99. doi: 10.1128/jb.153.1.93-99.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chamier B., Lorenz M. G., Wackernagel W. Natural Transformation of Acinetobacter calcoaceticus by Plasmid DNA Adsorbed on Sand and Groundwater Aquifer Material. Appl Environ Microbiol. 1993 May;59(5):1662–1667. doi: 10.1128/aem.59.5.1662-1667.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Datta N., Hedges R. W. Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae. J Gen Microbiol. 1972 Sep;72(2):349–355. doi: 10.1099/00221287-72-2-349. [DOI] [PubMed] [Google Scholar]
  6. Deich R. A., Smith H. O. Mechanism of homospecific DNA uptake in Haemophilus influenzae transformation. Mol Gen Genet. 1980 Feb;177(3):369–374. doi: 10.1007/BF00271475. [DOI] [PubMed] [Google Scholar]
  7. Dubnau D. The regulation of genetic competence in Bacillus subtilis. Mol Microbiol. 1991 Jan;5(1):11–18. doi: 10.1111/j.1365-2958.1991.tb01820.x. [DOI] [PubMed] [Google Scholar]
  8. Goodman S. D., Scocca J. J. Identification and arrangement of the DNA sequence recognized in specific transformation of Neisseria gonorrhoeae. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6982–6986. doi: 10.1073/pnas.85.18.6982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kahn M. E., Barany F., Smith H. O. Transformasomes: specialized membranous structures that protect DNA during Haemophilus transformation. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6927–6931. doi: 10.1073/pnas.80.22.6927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Khasanov F. K., Zvingila D. J., Zainullin A. A., Prozorov A. A., Bashkirov V. I. Homologous recombination between plasmid and chromosomal DNA in Bacillus subtilis requires approximately 70 bp of homology. Mol Gen Genet. 1992 Sep;234(3):494–497. doi: 10.1007/BF00538711. [DOI] [PubMed] [Google Scholar]
  11. Lorenz M. G., Wackernagel W. Bacterial gene transfer by natural genetic transformation in the environment. Microbiol Rev. 1994 Sep;58(3):563–602. doi: 10.1128/mr.58.3.563-602.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lorenz M. G., Wackernagel W. High Frequency of Natural Genetic Transformation of Pseudomonas stutzeri in Soil Extract Supplemented with a Carbon/Energy and Phosphorus Source. Appl Environ Microbiol. 1991 Apr;57(4):1246–1251. doi: 10.1128/aem.57.4.1246-1251.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morrison D. A., Guild W. R. Transformation and deoxyribonucleic acid size: extent of degradation on entry varies with size of donor. J Bacteriol. 1972 Dec;112(3):1157–1168. doi: 10.1128/jb.112.3.1157-1168.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Paul J. H., Frischer M. E., Thurmond J. M. Gene transfer in marine water column and sediment microcosms by natural plasmid transformation. Appl Environ Microbiol. 1991 May;57(5):1509–1515. doi: 10.1128/aem.57.5.1509-1515.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Prentki P., Krisch H. M. In vitro insertional mutagenesis with a selectable DNA fragment. Gene. 1984 Sep;29(3):303–313. doi: 10.1016/0378-1119(84)90059-3. [DOI] [PubMed] [Google Scholar]
  16. Rayssiguier C., Thaler D. S., Radman M. The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature. 1989 Nov 23;342(6248):396–401. doi: 10.1038/342396a0. [DOI] [PubMed] [Google Scholar]
  17. Shen P., Huang H. V. Homologous recombination in Escherichia coli: dependence on substrate length and homology. Genetics. 1986 Mar;112(3):441–457. doi: 10.1093/genetics/112.3.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sparling P. F. Genetic transformation of Neisseria gonorrhoeae to streptomycin resistance. J Bacteriol. 1966 Nov;92(5):1364–1371. doi: 10.1128/jb.92.5.1364-1371.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Spratt B. G., Zhang Q. Y., Jones D. M., Hutchison A., Brannigan J. A., Dowson C. G. Recruitment of a penicillin-binding protein gene from Neisseria flavescens during the emergence of penicillin resistance in Neisseria meningitidis. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8988–8992. doi: 10.1073/pnas.86.22.8988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stein D. C. Transformation of Neisseria gonorrhoeae: physical requirements of the transforming DNA. Can J Microbiol. 1991 May;37(5):345–349. doi: 10.1139/m91-056. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES