Abstract
A range of marine phytoplankton was grown in closed systems in order to investigate the kinetics of dissolved inorganic carbon (DIC) use and the influence of the nitrogen source under conditions of constant pH. The kinetics of DIC use could be described by a rectangular hyperbolic curve, yielding estimations of KG(DIC) (the half saturation constant for carbon-specific growth, i.e. C mu) and mu max (the theoretical maximum C mu). All species attained a KG(DIC) within the range of 30-750 microM DIC. For most species, NH4+ use enabled growth with a lower KG(DIC) and/or, for two species, an increase in mu max. At DIC concentrations of > 1.6 mM, C mu was > 90% saturated for all species relative to the rate at the natural seawater DIC concentration of 2.0 mM. The results suggest that neither the rate nor the extent of primary productivity will be significantly limited by the DIC in the quasi-steady-state conditions associated with oligotrophic oceans. The method needs to be applied in the conditions associated with dynamic coastal (eutrophic) systems for clarification of a potential DIC rate limitation where cells may grow to higher densities and under variable pH and nitrogen supply.
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- Badger M. R., Kaplan A., Berry J. A. Internal Inorganic Carbon Pool of Chlamydomonas reinhardtii: EVIDENCE FOR A CARBON DIOXIDE-CONCENTRATING MECHANISM. Plant Physiol. 1980 Sep;66(3):407–413. doi: 10.1104/pp.66.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boklage C. E. Sex ratio unaffected by parental age gap. Nature. 1997 Nov 20;390(6657):243–243. doi: 10.1038/36765. [DOI] [PubMed] [Google Scholar]
- Goldman J. C., Graham S. J. Inorganic carbon limitation and chemical composition of two freshwater green microalgae. Appl Environ Microbiol. 1981 Jan;41(1):60–70. doi: 10.1128/aem.41.1.60-70.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller A. G., Colman B. Evidence for HCO(3) Transport by the Blue-Green Alga (Cyanobacterium) Coccochloris peniocystis. Plant Physiol. 1980 Feb;65(2):397–402. doi: 10.1104/pp.65.2.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller A. G., Turpin D. H., Canvin D. T. Growth and Photosynthesis of the Cyanobacterium Synechococcus leopoliensis in HCO(3)-Limited Chemostats. Plant Physiol. 1984 Aug;75(4):1064–1070. doi: 10.1104/pp.75.4.1064. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rau G. H., Takahashi T., Des Marais D. J. Latitudinal variations in plankton delta 13C: implications for CO2 and productivity in past oceans. Nature. 1989 Oct 12;341(6242):516–518. doi: 10.1038/341516a0. [DOI] [PubMed] [Google Scholar]
- Raven J. A. Nutrient transport in microalgae. Adv Microb Physiol. 1980;21:47–226. doi: 10.1016/s0065-2911(08)60356-2. [DOI] [PubMed] [Google Scholar]
- Read B. A., Tabita F. R. High substrate specificity factor ribulose bisphosphate carboxylase/oxygenase from eukaryotic marine algae and properties of recombinant cyanobacterial RubiSCO containing "algal" residue modifications. Arch Biochem Biophys. 1994 Jul;312(1):210–218. doi: 10.1006/abbi.1994.1301. [DOI] [PubMed] [Google Scholar]
- Suzuki K., Spalding M. H. Adaptation of Chlamydomonas reinhardtii High-CO(2)-Requiring Mutants to Limiting CO(2). Plant Physiol. 1989 Jul;90(3):1195–1200. doi: 10.1104/pp.90.3.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]