Abstract
The evolutionary relationships of the burrowing amphisbaenians ('worm lizards') have long been controversial for several reasons: the rarity of museum specimens available for study, highly derived morphological conditions that can confound comparative studies and difficulty in obtaining tissues for molecular phylogenetic studies because of their secretive habits in the wild. We present a phylogenetic analysis of two nuclear genes obtained from both fresh tissues and museum specimens of worm lizards. We achieved sufficient taxonomic sampling for analysis by extracting DNA from museum specimens using a modified forensics protocol. Results show the limbless Rhineuridae to be the most basal lineage, whereas the limbed Bipedidae occupy a more derived position as the sister-taxon to a Trogonophidae-Amphisbaenidae clade. This pattern of relationships indicates widespread morphological convergence within the group, including three independent incidences of limb loss. Convergence in skull shape and scalation is also prevalent. Mosaic evolution in the skull versus postcranial skeleton parallels that seen in snake evolution.
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- doi: 10.1098/rstb.1998.0308. [DOI] [PMC free article] [Google Scholar]
- Posada D., Crandall K. A. MODELTEST: testing the model of DNA substitution. Bioinformatics. 1998;14(9):817–818. doi: 10.1093/bioinformatics/14.9.817. [DOI] [PubMed] [Google Scholar]
- Saint K. M., Austin C. C., Donnellan S. C., Hutchinson M. N. C-mos, a nuclear marker useful for squamate phylogenetic analysis. Mol Phylogenet Evol. 1998 Oct;10(2):259–263. doi: 10.1006/mpev.1998.0515. [DOI] [PubMed] [Google Scholar]
- Shedlock A. M., Haygood M. G., Pietsch T. W., Bentzen P. Enhanced DNA extraction and PCR amplification of mitochondrial genes from formalin-fixed museum specimens. Biotechniques. 1997 Mar;22(3):394-6, 398, 400. doi: 10.2144/97223bm03. [DOI] [PubMed] [Google Scholar]
- Tchernov E., Rieppel O., Zaher H., Polcyn M. J., Jacobs L. L. A fossil snake with limbs. Science. 2000 Mar 17;287(5460):2010–2012. doi: 10.1126/science.287.5460.2010. [DOI] [PubMed] [Google Scholar]
- Vidal Nicolas, Hedges S. Blair. Molecular evidence for a terrestrial origin of snakes. Proc Biol Sci. 2004 May 7;271 (Suppl 4):S226–S229. doi: 10.1098/rsbl.2003.0151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whiting Alison S., Bauer Aaron M., Sites Jack W., Jr Phylogenetic relationships and limb loss in sub-Saharan African scincine lizards (Squamata: Scincidae). Mol Phylogenet Evol. 2003 Dec;29(3):582–598. doi: 10.1016/s1055-7903(03)00142-8. [DOI] [PubMed] [Google Scholar]
- Wiens J. J., Slingluff J. L. How lizards turn into snakes: a phylogenetic analysis of body-form evolution in anguid lizards. Evolution. 2001 Nov 11;55(11):2303–2318. doi: 10.1111/j.0014-3820.2001.tb00744.x. [DOI] [PubMed] [Google Scholar]
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