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. 1993 Dec;83(12):1754–1756. doi: 10.2105/ajph.83.12.1754

Neonatal tetanus in Peru: risk assessment with modified enzyme-linked immunosorbent assay and toxoid skin test.

L Vernacchio 1, G Madico 1, M Verastegui 1, F Diaz 1, T S Collins 1, R H Gilman 1
PMCID: PMC1694911  PMID: 8259811

Abstract

We used a modified enzyme-linked immunosorbent assay (ELISA) to investigate tetanus immunity in 232 pregnant Peruvian women. One hundred forty-two (61.2%) had protective antitoxin titers (> or = 0.01 IU/mL). Protective titers correlated positively with the number of toxoid doses reported during the current pregnancy. A majority of women reporting no toxoid doses during the current pregnancy had at least one prenatal health care visit. We evaluated a toxoid skin test in 44 of the subjects, but it correlated poorly with the ELISA. The modified ELISA is a useful in vitro method for studying tetanus immunity in the developing world.

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Selected References

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  1. Bhatia S., Ray K., Mathur R., Yadav T. P., Rai Chowdhary A. N., Grover S. S., Narayanan I. Evaluation of cutaneous response to tetanus antigen to document immunization: preliminary observations. Indian Pediatr. 1985 Apr;22(4):267–273. [PubMed] [Google Scholar]
  2. Black R. E., Huber D. H., Curlin G. T. Reduction of neonatal tetanus by mass immunization of non-pregnant women: duration of protection provided by one or two doses of aluminium-adsorbed tetanus toxoid. Bull World Health Organ. 1980;58(6):927–930. [PMC free article] [PubMed] [Google Scholar]
  3. Chen S. T., Edsall G., Peel M. M., Sinnathuray T. A. Timing of antenatal tetanus immunization for effective protection of the neonate. Bull World Health Organ. 1983;61(1):159–165. [PMC free article] [PubMed] [Google Scholar]
  4. Dastur F. D., Awatramani V. P., Dixit S. K., D'Sa J. A., Cooverji N. D., Anand M. P. Response to single dose of tetanus vaccine in subjects with naturally acquired tetanus antitoxin. Lancet. 1981 Aug 1;2(8240):219–222. doi: 10.1016/s0140-6736(81)90472-4. [DOI] [PubMed] [Google Scholar]
  5. Facktor M. A., Bernstein R. A., Fireman P. Hypersensitivity to tetanus toxoid. J Allergy Clin Immunol. 1973 Jul;52(1):1–12. doi: 10.1016/0091-6749(73)90115-2. [DOI] [PubMed] [Google Scholar]
  6. Newell K. W., Dueñas Lehmann A., LeBlanc D. R., Garces Osorio N. The use of toxoid for the prevention of tetanus neonatorum. Final report of a double-blind controlled field trial. Bull World Health Organ. 1966;35(6):863–871. [PMC free article] [PubMed] [Google Scholar]
  7. Ray S. N., Ray K., Grover S. S., Sharma R. S., Sharma S. P. Sero-survey of diphtheria and tetanus antitoxin. Indian J Med Res. 1978 Dec;68:901–904. [PubMed] [Google Scholar]
  8. Réthy L., Réthy L. A. Active anti-tetanus immunisation of females to control neonatal tetanus. Lancet. 1986 Mar 15;1(8481):616–616. doi: 10.1016/s0140-6736(86)92835-7. [DOI] [PubMed] [Google Scholar]
  9. Simonsen O., Bentzon M. W., Heron I. ELISA for the routine determination of antitoxic immunity to tetanus. J Biol Stand. 1986 Jul;14(3):231–239. doi: 10.1016/0092-1157(86)90008-9. [DOI] [PubMed] [Google Scholar]
  10. Simonsen O., Kjeldsen K., Heron I. Immunity against tetanus and effect of revaccination 25-30 years after primary vaccination. Lancet. 1984 Dec 1;2(8414):1240–1242. doi: 10.1016/s0140-6736(84)92796-x. [DOI] [PubMed] [Google Scholar]
  11. Simonsen O., Schou C., Heron I. Modification of the ELISA for the estimation of tetanus antitoxin in human sera. J Biol Stand. 1987 Apr;15(2):143–157. doi: 10.1016/0092-1157(87)90037-0. [DOI] [PubMed] [Google Scholar]
  12. Sridama V., Pacini F., Yang S. L., Moawad A., Reilly M., DeGroot L. J. Decreased levels of helper T cells: a possible cause of immunodeficiency in pregnancy. N Engl J Med. 1982 Aug 5;307(6):352–356. doi: 10.1056/NEJM198208053070606. [DOI] [PubMed] [Google Scholar]
  13. Thong Y. H., Steele R. W., Vincent M. M., Hensen S. A., Bellanti J. A. Impaired in vitro cell-mediated immunity to rubella virus during pregnancy. N Engl J Med. 1973 Sep 20;289(12):604–606. doi: 10.1056/NEJM197309202891203. [DOI] [PubMed] [Google Scholar]
  14. Veronesi R., Cecin H., Corrêa A., Tavares J., Moraes C., Bertoldo O. J. New concepts on tetanus immunization: naturally acquired immunity. J Hyg Epidemiol Microbiol Immunol. 1975;19(1):126–134. [PubMed] [Google Scholar]
  15. da Silveira C. M., de Quadros C. A. Neonatal tetanus: countdown to 1995. World Health Forum. 1991;12(3):289–296. [PubMed] [Google Scholar]

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