Abstract
The Hungate anaerobic technique was used with a standard procedure for bacterial mutagenesis employing N-methyl-N-nitro-N'-nitrosoguanidine to obtain mutants of an obligate anaerobe. Three mutant strains were derived from a Coprococcus sp., strain Pe15, a rumen anaerobe capable of growing on phloroglucinol. The mutants did not grow on phloroglucinol but did degrade the compound in anaerobic washed-cell suspensions, producing the same end products in approximately the same proportions as the wild type. It was concluded that the mutants were blocked in a unique step or steps necessary for carbon skeleton or energy synthesis from phloroglucinol and not in formation of an enzyme involved in the pathway of phloroglucinol degradation.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Caldwell D. R., Bryant M. P. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol. 1966 Sep;14(5):794–801. doi: 10.1128/am.14.5.794-801.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cheng K. J., Krishnamurty H. G., Jones G. A., Simpson F. J. Identification of products produced by the anaerobic degradation of naringin by Butyrivibrio sp. C3. Can J Microbiol. 1971 Jan;17(1):129–131. doi: 10.1139/m71-022. [DOI] [PubMed] [Google Scholar]
- DEHORITY B. A., JOHNSON R. R., BENTLEY O. G., MOXON A. L. Studies on the metabolism of valine, proline, leucine and isoleucine by rumen microorganisms in vitro. Arch Biochem Biophys. 1958 Nov;78(1):15–27. doi: 10.1016/0003-9861(58)90310-2. [DOI] [PubMed] [Google Scholar]
- Dagley S. Catabolism of aromatic compounds by micro-organisms. Adv Microb Physiol. 1971;6(0):1–46. doi: 10.1016/s0065-2911(08)60066-1. [DOI] [PubMed] [Google Scholar]
- Dutton P. L., Evans W. C. The metabolism of aromatic compounds by Rhodopseudomonas palustris. A new, reductive, method of aromatic ring metabolism. Biochem J. 1969 Jul;113(3):525–536. doi: 10.1042/bj1130525. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EVANS W. C. THE MICROBIOLOGICAL DEGRADATION OF AROMATIC COMPOUNDS. J Gen Microbiol. 1963 Aug;32:177–184. doi: 10.1099/00221287-32-2-177. [DOI] [PubMed] [Google Scholar]
- FINA L. R., FISKIN A. M. The anaerobic decomposition of benzoic acid during methane fermentation. II. Fate of carbons one and seven. Arch Biochem Biophys. 1960 Dec;91:163–165. doi: 10.1016/0003-9861(60)90483-5. [DOI] [PubMed] [Google Scholar]
- Guyer M., Hegeman G. Evidence for a reductive pathway for the anaerobic metabolism of benzoate. J Bacteriol. 1969 Sep;99(3):906–907. doi: 10.1128/jb.99.3.906-907.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leng R. A., Brett D. J. Simultaneous measurements of the rates of production of acetic, propionic and butyric acids in the rumen of sheep on different diets and the correlation between production rates and concentrations of these acids in the rumen. Br J Nutr. 1966;20(3):541–552. doi: 10.1079/bjn19660053. [DOI] [PubMed] [Google Scholar]
- Nottingham P. M., Hungate R. E. Methanogenic fermentation of benzoate. J Bacteriol. 1969 Jun;98(3):1170–1172. doi: 10.1128/jb.98.3.1170-1172.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ornston L. N. The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida. IV. Regulation. J Biol Chem. 1966 Aug 25;241(16):3800–3810. [PubMed] [Google Scholar]
- Sebald M., Costilow R. N. Minimal growth requirements for Clostridium perfringens and isolation of auxotrophic mutants. Appl Microbiol. 1975 Jan;29(1):1–6. doi: 10.1128/am.29.1.1-6.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor B. F., Campbell W. L., Chinoy I. Anaerobic degradation of the benzene nucleus by a facultatively anaerobic microorganism. J Bacteriol. 1970 May;102(2):430–437. doi: 10.1128/jb.102.2.430-437.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsai C. G., Jones G. A. Isolation and identification of rumen bacteria capable of anaerobic phloroglucinol degradation. Can J Microbiol. 1975 Jun;21(6):794–801. doi: 10.1139/m75-117. [DOI] [PubMed] [Google Scholar]