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. 1976 Sep;32(3):376–380. doi: 10.1128/aem.32.3.376-380.1976

Silica gel medium to detect molds that produce aflatoxin.

G S Torrey, E H Marth
PMCID: PMC170073  PMID: 185957

Abstract

A chemically defined nutrient solution containing tartaric acid was made solid by mixing it with an alkaline silicate solution. Production of aflatoxin by asperfilli growing on the silica gel medium coincided with the presence of a blue fluorescent area surrounding colonies, as observed under long-wave ultraviolet light. Presence of aflatoxin in the medium was confirmed by drying the gels, extracting them with chloroform-methanol, and examining extracts for fluorescent materials by viewing them on thin-layer chromatograms under ultraviolet light.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davis N. D., Diener U. L., Agnihotri V. P. Production of aflatoxins B1 and G1 in chemically defined medium. Mycopathol Mycol Appl. 1967 Apr 28;31(3):251–256. doi: 10.1007/BF02053422. [DOI] [PubMed] [Google Scholar]
  2. FUNK H. B., KRULWICH T. A. PREPARATION OF CLEAR SILICA GELS THAT CAN BE STREAKED. J Bacteriol. 1964 Oct;88:1200–1201. doi: 10.1128/jb.88.4.1200-1201.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hara S., Fennell D. I., Hesseltine C. W. Aflatoxin-producing strains of Aspergillus flavus detected by fluorescence of agar medium under ultraviolet light. Appl Microbiol. 1974 Jun;27(6):1118–1123. doi: 10.1128/am.27.6.1118-1123.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. MATELES R. I., ADYE J. C. PRODUCTION OF AFLATOXINS IN SUBMERGED CULTURE. Appl Microbiol. 1965 Mar;13:208–211. doi: 10.1128/am.13.2.208-211.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Reddy T. V., Viswanathan L., Venkitasubramanian T. A. High aflatoxin production on a chemically defined medium. Appl Microbiol. 1971 Sep;22(3):393–396. doi: 10.1128/am.22.3.393-396.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. de Vogel P., van Rhee R., Blanche Koelensmid W. A. A rapid screening test for aflatoxin-synthesizing aspergilli of the flavus-oryzae group. J Appl Bacteriol. 1965 Aug;28(2):213–220. doi: 10.1111/j.1365-2672.1965.tb02145.x. [DOI] [PubMed] [Google Scholar]

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