Skip to main content
PMC home page
Clinical and Diagnostic Laboratory Immunology logoLink to Clinical and Diagnostic Laboratory Immunology
. 1995 Jan;2(1):122–124. doi: 10.1128/cdli.2.1.122-124.1995

Investigation of cross-reactions against Trichinella spiralis antigens by enzyme-linked immunosorbent assay and enzyme-linked immunoelectrotransfer blot assay in patients with various diseases.

J L De-la-Rosa 1, P Alcantara 1, D Correa 1
PMCID: PMC170113  PMID: 7719905

Abstract

Data regarding cross-reactions against Trichinella spiralis in humans are scarce and controversial. For this reason, we tested serum samples from patients with typhoid fever, brucellosis, toxoplasmosis, amoebiasis, cysticercosis, trichocephaliasis, ascariasis, and onchocerciasis against an antigenic extract of T. spiralis infective larvae in an enzyme-linked immunosorbent assay (ELISA) and an enzyme-linked immunoelectrotransfer blot (EITB) assay. All except one serum sample from the group of patients with onchocerciasis were negative in the ELISA; in the EITB assay, only faint bands were observed with the samples from patients with onchocerciasis and ascariasis and negative results were obtained with the samples from patients with other diseases. In conclusion, cross-reactions were found only in the groups of patients with other nematode infections and were of very low magnitude, most of them virtually negative.

Full Text

The Full Text of this article is available as a PDF (306.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alcántara P., Correa D. Human humoral immune responses against Trichinella spiralis. Int J Parasitol. 1993 Aug;23(5):657–660. doi: 10.1016/0020-7519(93)90173-v. [DOI] [PubMed] [Google Scholar]
  2. Arriaga C., Muñiz E., Morilla A., Ortega-Pierres G. Trichinella spiralis: recognition of muscle larva antigens during experimental infection of swine and its potential use in diagnosis. Exp Parasitol. 1989 Nov;69(4):363–372. doi: 10.1016/0014-4894(89)90086-6. [DOI] [PubMed] [Google Scholar]
  3. Au A. C., Ko R. C., Simon J. W., Ridell N. J., Wong F. W., Templer M. J. Study of acute trichinosis in Ghurkas: specificity and sensitivity of enzyme-linked immunosorbent assays for IgM and IgE antibodies to Trichinella larval antigens in diagnosis. Trans R Soc Trop Med Hyg. 1983;77(3):412–415. doi: 10.1016/0035-9203(83)90175-x. [DOI] [PubMed] [Google Scholar]
  4. Barriga O. O. Reactivity and specificity of Trichinella spiralis fractions in cutaneous and serological tests. J Clin Microbiol. 1977 Sep;6(3):274–279. doi: 10.1128/jcm.6.3.274-279.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bruschi F., Tassi C., Pozio E. Parasite-specific antibody response in Trichinella sp. 3 human infection: a one year follow-up. Am J Trop Med Hyg. 1990 Aug;43(2):186–193. doi: 10.4269/ajtmh.1990.43.186. [DOI] [PubMed] [Google Scholar]
  6. Fragoso Uribe R. Un brote de triquinosis en Villanueva, Zacatecas. Salud Publica Mex. 1981 Jan-Feb;23(1):25–41. [PubMed] [Google Scholar]
  7. Gamble H. R. Application of hybridoma technology to the diagnosis of parasitic disease. Vet Parasitol. 1984 Jun;14(3-4):251–261. doi: 10.1016/0304-4017(84)90095-5. [DOI] [PubMed] [Google Scholar]
  8. Ikegaki N., Kennett R. H. Glutaraldehyde fixation of the primary antibody-antigen complex on nitrocellulose paper increases the overall sensitivity of immunoblot assay. J Immunol Methods. 1989 Nov 30;124(2):205–210. doi: 10.1016/0022-1759(89)90354-2. [DOI] [PubMed] [Google Scholar]
  9. Kolata G. Testing for trichinosis. Science. 1985 Feb 8;227(4687):621–624. doi: 10.1126/science.3969551. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Martínez Marañn R. Está aumentando la triquinosis en México? Podría esto ser una consecuencia inesperada de nuestro "desarrollo"? Salud Publica Mex. 1985 Jan-Feb;27(1):40–51. [PubMed] [Google Scholar]
  12. Martínez Marañon R. Triquinosis en Zacatecas, Zac. Estudio epidemiológico y clińico. Prensa Med Mex. 1979 Nov-Dec;44(11-12):278–287. [PubMed] [Google Scholar]
  13. Morakote N., Khamboonruang C., Siriprasert V., Suphawitayanukul S., Marcanantachoti S., Thamasonthi W. The value of enzyme-linked immunosorbent assay (ELISA) for diagnosis of human trichinosis. Trop Med Parasitol. 1991 Sep;42(3):172–174. [PubMed] [Google Scholar]
  14. Ortega-Pierres M. G., Almond N. W., Parkhouse R. M. Identification of biochemically defined antigens of Trichinella spiralis in host immunity and in diagnosis. Wiad Parazytol. 1987;33(4-5):423–452. [PubMed] [Google Scholar]
  15. PRICE S., WEINER L. M. A study of antigenic relationships between Trichinella spiralis and Salmonella typhi. J Immunol. 1956 Aug;77(2):111–114. [PubMed] [Google Scholar]
  16. Parkhouse R. M., Philipp M., Ogilvie B. M. Characterization of surface antigens of Trichinella spiralis infective larvae. Parasite Immunol. 1981 Winter;3(4):339–352. doi: 10.1111/j.1365-3024.1981.tb00412.x. [DOI] [PubMed] [Google Scholar]
  17. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Zarlenga D. S., Gamble H. R. Molecular cloning and expression of an immunodominant 53-kDa excretory-secretory antigen from Trichinella spiralis muscle larvae. Mol Biochem Parasitol. 1990 Sep-Oct;42(2):165–174. doi: 10.1016/0166-6851(90)90159-j. [DOI] [PubMed] [Google Scholar]
  19. van Knapen F., Franchimont J. H., Skovgaard N., Guil'dal J., Henriksen S. A. Husbandry, parasitic and other diseases as factors in the reliability of the enzyme linked immunosorbent assay (ELISA) for detection of trichinellosis in pigs. Vet Parasitol. 1984 Oct;16(1-2):17–22. doi: 10.1016/0304-4017(84)90004-9. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Diagnostic Laboratory Immunology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES