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. 1995 Mar;2(2):149–155. doi: 10.1128/cdli.2.2.149-155.1995

Cross-reactive antigens shared by Pseudomonas aeruginosa, Helicobacter pylori, Campylobacter jejuni, and Haemophilus influenzae may cause false-positive titers of antibody to H. pylori.

H K Johansen 1, A Nørgaard 1, L P Andersen 1, P Jensen 1, H Nielsen 1, N Høiby 1
PMCID: PMC170118  PMID: 7697522

Abstract

Cystic fibrosis (CF) patients suffer from many of the gastrointestinal conditions which occur in non-CF individuals, e.g., dyspepsia and peptic ulceration. These symptoms may be caused by Helicobacter pylori but could also be due to either pancreatic insufficiency or the intensive antibiotic treatment used in CF patients. Since CF patients chronically infected with Pseudomonas aeruginosa produce antibodies against a wide range of antigens, including antigens common to many other bacteria, e.g., GroEL and lipopolysaccharide, we studied, by the Western blot (immunoblot) technique, the specificity of immunoglobulin G antibodies to H. pylori in Danish CF patients chronically infected with P. aeruginosa, CF patients without P. aeruginosa infection but with Haemophilus influenzae infection, patients with dyspeptic ulcers associated with H. pylori, and patients recovering from acute Campylobacter jejuni or Campylobacter coli infection. Sera from CF patients with chronic P. aeruginosa or H. influenzae infection and patients recovering from acute C. jejuni infection cross-reacted with H. pylori antigens. A strong cross-reacting protein antigen at approximately 14 kDa and minor cross-reactive antigens at approximately 27, 30, and 60 kDa (the heat shock protein GroEL is equivalent to the common antigen of P. aeruginosa) could be demonstrated. The results of this study show that high immunoglobulin G antibody titers against H. pylori in CF patients cannot be regarded as indicating present or past H. pylori infection unless their specificity is proven by absorption studies.

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Selected References

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  1. Andersen L. P., Espersen F. Immunoglobulin G antibodies to Helicobacter pylori in patients with dyspeptic symptoms investigated by the western immunoblot technique. J Clin Microbiol. 1992 Jul;30(7):1743–1751. doi: 10.1128/jcm.30.7.1743-1751.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andersen L. P., Gaarslev K. IgG subclass antibodies against Helicobacter pylori heat-stable antigens in normal persons and in dyspeptic patients. APMIS. 1992 Aug;100(8):747–751. [PubMed] [Google Scholar]
  3. Andersen L. P., Holck S., Povlsen C. O. Campylobacter pylori detected by indirect immunohistochemical technique. APMIS. 1988 Jun;96(6):559–564. [PubMed] [Google Scholar]
  4. Andersen L. P., Holck S., Povlsen C. O., Elsborg L., Justesen T. Campylobacter pyloridis in peptic ulcer disease. I. Gastric and duodenal infection caused by C. pyloridis: histopathologic and microbiologic findings. Scand J Gastroenterol. 1987 Mar;22(2):219–224. doi: 10.3109/00365528708991883. [DOI] [PubMed] [Google Scholar]
  5. Bangsborg J. M., Shand G., Pearlman E., Høiby N. Cross-reactive Legionella antigens and the antibody response during infection. APMIS. 1991 Sep;99(9):854–865. [PubMed] [Google Scholar]
  6. Collins M. T., McDonald J., Høiby N., Aalund O. Agglutinating antibody titers to members of the family Legionellaceae in cystic fibrosis patients as a result of cross-reacting antibodies to Pseudomonas aeruginosa. J Clin Microbiol. 1984 Jun;19(6):757–762. doi: 10.1128/jcm.19.6.757-762.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crabtree J. E., Taylor J. D., Wyatt J. I., Heatley R. V., Shallcross T. M., Tompkins D. S., Rathbone B. J. Mucosal IgA recognition of Helicobacter pylori 120 kDa protein, peptic ulceration, and gastric pathology. Lancet. 1991 Aug 10;338(8763):332–335. doi: 10.1016/0140-6736(91)90477-7. [DOI] [PubMed] [Google Scholar]
  8. Döring G., Høiby N. Longitudinal study of immune response to Pseudomonas aeruginosa antigens in cystic fibrosis. Infect Immun. 1983 Oct;42(1):197–201. doi: 10.1128/iai.42.1.197-201.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Espersen F., Clemmensen I., Barkholt V. Isolation of Staphylococcus aureus clumping factor. Infect Immun. 1985 Sep;49(3):700–708. doi: 10.1128/iai.49.3.700-708.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fernandes P. B., Kim C., Cundy K. R., Haung N. N. Antibodies to cell envelope proteins of Pseudomonas aeruginosa in cystic fibrosis patients. Infect Immun. 1981 Aug;33(2):527–532. doi: 10.1128/iai.33.2.527-532.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fomsgaard A., Shand G. H., Freudenberg M. A., Galanos C., Conrad R. S., Kronborg G., Høiby N. Antibodies from chronically infected cystic fibrosis patients react with lipopolysaccharides extracted by new micromethods from all serotypes of Pseudomonas aeruginosa. APMIS. 1993 Feb;101(2):101–112. doi: 10.1111/j.1699-0463.1993.tb00088.x. [DOI] [PubMed] [Google Scholar]
  12. Fyfe J. A., Govan J. R. Alginate synthesis in mucoid Pseudomonas aeruginosa: a chromosomal locus involved in control. J Gen Microbiol. 1980 Aug;119(2):443–450. doi: 10.1099/00221287-119-2-443. [DOI] [PubMed] [Google Scholar]
  13. Govan J. R., Fyfe J. A. Mucoid Pseudomonas aeruginosa and cystic fibrosis: resistance of the mucoid from to carbenicillin, flucloxacillin and tobramycin and the isolation of mucoid variants in vitro. J Antimicrob Chemother. 1978 May;4(3):233–240. doi: 10.1093/jac/4.3.233. [DOI] [PubMed] [Google Scholar]
  14. Granström M., Ericsson A., Strandvik B., Wretlind B., Pavlovskis O. R., Berka R., Vasil M. L. Relation between antibody response to Pseudomonas aeruginosa exoproteins and colonization/infection in patients with cystic fibrosis. Acta Paediatr Scand. 1984 Nov;73(6):772–777. doi: 10.1111/j.1651-2227.1984.tb17774.x. [DOI] [PubMed] [Google Scholar]
  15. Hancock R. E., Mouat E. C., Speert D. P. Quantitation and identification of antibodies to outer-membrane proteins of Pseudomonas aeruginosa in sera of patients with cystic fibrosis. J Infect Dis. 1984 Feb;149(2):220–226. doi: 10.1093/infdis/149.2.220. [DOI] [PubMed] [Google Scholar]
  16. Høiby N., Pedersen S. S. Estimated risk of cross-infection with Pseudomonas aeruginosa in Danish cystic fibrosis patients. Acta Paediatr Scand. 1989 May;78(3):395–404. doi: 10.1111/j.1651-2227.1989.tb11099.x. [DOI] [PubMed] [Google Scholar]
  17. Jensen P., Fomsgaard A., Shand G., Hindersson P., Høiby N. Antigenic analysis of Pseudomonas aeruginosa and Pseudomonas cepacia GroEL proteins and demonstration of a lipopolysaccharide-associated GroEL fraction in P. aeruginosa. APMIS. 1993 Aug;101(8):621–630. doi: 10.1111/j.1699-0463.1993.tb00156.x. [DOI] [PubMed] [Google Scholar]
  18. Johansen H. K., Espersen F., Pedersen S. S., Hougen H. P., Rygaard J., Høiby N. Chronic Pseudomonas aeruginosa lung infection in normal and athymic rats. APMIS. 1993 Mar;101(3):207–225. [PubMed] [Google Scholar]
  19. Klinger J. D., Straus D. C., Hilton C. B., Bass J. A. Antibodies to proteases and exotoxin A of Pseudomonas aeruginosa in patients with cystic fibrosis: Demonstration by radioimmunoassay. J Infect Dis. 1978 Jul;138(1):49–48. doi: 10.1093/infdis/138.1.49. [DOI] [PubMed] [Google Scholar]
  20. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  21. Littlewood J. M. Cystic fibrosis: gastrointestinal complications. Br Med Bull. 1992 Oct;48(4):847–859. doi: 10.1093/oxfordjournals.bmb.a072581. [DOI] [PubMed] [Google Scholar]
  22. Newell D. G. Identification of the outer membrane proteins of Campylobacter pyloridis and antigenic cross-reactivity between C. pyloridis and C. jejuni. J Gen Microbiol. 1987 Jan;133(1):163–170. doi: 10.1099/00221287-133-1-163. [DOI] [PubMed] [Google Scholar]
  23. Pedersen S. S., Jensen T., Høiby N., Koch C., Flensborg E. W. Management of Pseudomonas aeruginosa lung infection in Danish cystic fibrosis patients. Acta Paediatr Scand. 1987 Nov;76(6):955–961. doi: 10.1111/j.1651-2227.1987.tb17271.x. [DOI] [PubMed] [Google Scholar]
  24. Pedersen S. S. Lung infection with alginate-producing, mucoid Pseudomonas aeruginosa in cystic fibrosis. APMIS Suppl. 1992;28:1–79. [PubMed] [Google Scholar]
  25. Perez-Perez G. I., Blaser M. J. Conservation and diversity of Campylobacter pyloridis major antigens. Infect Immun. 1987 May;55(5):1256–1263. doi: 10.1128/iai.55.5.1256-1263.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Perez-Perez G. I., Hopkins J. A., Blaser M. J. Lipopolysaccharide structures in Enterobacteriaceae, Pseudomonas aeruginosa, and Vibrio cholerae are immunologically related to Campylobacter spp. Infect Immun. 1986 Jan;51(1):204–208. doi: 10.1128/iai.51.1.204-208.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Phelan M. S., Fine D. R., Zentler-Munro P. L., Hodson M. E., Batten J. C., Kerr I. H. Radiographic abnormalities of the duodenum in cystic fibrosis. Clin Radiol. 1983 Sep;34(5):573–577. doi: 10.1016/s0009-9260(83)80168-8. [DOI] [PubMed] [Google Scholar]
  28. Rathbone B. J., Wyatt J. I., Worsley B. W., Shires S. E., Trejdosiewicz L. K., Heatley R. V., Losowsky M. S. Systemic and local antibody responses to gastric Campylobacter pyloridis in non-ulcer dyspepsia. Gut. 1986 Jun;27(6):642–647. doi: 10.1136/gut.27.6.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Schiøtz P. O., Høiby N., Hertz J. B. Cross-reactions between Haemophilus influenzae and nineteen other bacterial species. Acta Pathol Microbiol Scand B. 1979 Dec;87(6):337–344. doi: 10.1111/j.1699-0463.1979.tb02448.x. [DOI] [PubMed] [Google Scholar]
  30. Schiøtz P. O., Høiby N. Precipitating antibodies against Haemophilus influenzae and Staphylococcus aureus in sputum and serum from patients with cystic fibrosis. Acta Pathol Microbiol Scand B. 1979 Dec;87(6):345–351. doi: 10.1111/j.1699-0463.1979.tb02449.x. [DOI] [PubMed] [Google Scholar]
  31. Shand G. H., Pedersen S. S., Tilling R., Brown M. R., Høiby N. Use of immunoblot detection of serum antibodies in the diagnosis of chronic Pseudomonas aeruginosa lung infection in cystic fibrosis. J Med Microbiol. 1988 Nov;27(3):169–177. doi: 10.1099/00222615-27-3-169. [DOI] [PubMed] [Google Scholar]
  32. Sipos A., Klocke M., Frosch M. Cloning and sequencing of the genes coding for the 10- and 60-kDa heat shock proteins from Pseudomonas aeruginosa and mapping of a species-specific epitope. Infect Immun. 1991 Sep;59(9):3219–3226. doi: 10.1128/iai.59.9.3219-3226.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sompolinsky D., Hertz J. B., Høiby N., Jensen K., Mansa B., Samra Z. An antigen common to a wide range of bacteria. I. The isolation of a 'common antigen' from Pseudomonas aeruginosa. Acta Pathol Microbiol Scand B. 1980 Jun;88(3):143–149. doi: 10.1111/j.1699-0463.1980.tb02620.x. [DOI] [PubMed] [Google Scholar]
  34. Taussig L. M., Saldino R. M., Di Sant'Agnese P. A. Radiographic abnormalities of the duodenum and small bowel in cystic fibrosis of the pancreas (mucoviscidosis). Radiology. 1973 Feb;106(2):369–376. doi: 10.1148/106.2.369. [DOI] [PubMed] [Google Scholar]
  35. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Valerius N. H., Koch C., Høiby N. Prevention of chronic Pseudomonas aeruginosa colonisation in cystic fibrosis by early treatment. Lancet. 1991 Sep 21;338(8769):725–726. doi: 10.1016/0140-6736(91)91446-2. [DOI] [PubMed] [Google Scholar]

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