Skip to main content
NCBI home page
British Medical Journal logoLink to British Medical Journal
. 1970 Aug 29;3(5721):495–497. doi: 10.1136/bmj.3.5721.495

Sulphadimidine Acetylation Test for Classification of Patients as Slow or Rapid Inactivators of Isoniazid

K V N Rao, D A Mitchison, N G K Nair, K Prema, S P Tripathy
PMCID: PMC1701336  PMID: 5454342

Abstract

Sulphadimidine acetylation studies were undertaken in 103 patients, 52 of whom had been classified as slow and 51 as rapid inactivators of isoniazid by a standard microbiological assay method. Each patient received sulphadimidine by mouth in a dose of 44 mg./kg. body weight, and free and total sulphadimidine were estimated in blood and urine collected at six hours. The findings suggest that patients may be classified as slow inactivators of isoniazid if the proportion of acetylated sulphadimidine (total minus free) is (a) less than 25% in blood or (b) less than 70% in urine. The sulphadimidine test is easy to perform and the result is available the same day; urine specimens for the test can be stored at room temperature for over a week without any loss of drug.

Full text

PDF
495

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EIDUS L., HAMILTON E. J. A NEW METHOD FOR THE DETERMINATION OF N-ACETYL ISONIAZID IN URINE OF AMBULATORY PATIENTS. Am Rev Respir Dis. 1964 Apr;89:587–588. doi: 10.1164/arrd.1964.89.4.587. [DOI] [PubMed] [Google Scholar]
  2. EVANS D. A. INDIVIDUAL VARIATIONS OF DRUG METABOLISM AS A FACTOR IN DRUG TOXICITY. Ann N Y Acad Sci. 1965 Mar 12;123:178–187. doi: 10.1111/j.1749-6632.1965.tb12255.x. [DOI] [PubMed] [Google Scholar]
  3. EVANS D. A., MANLEY K. A., McKUSICK V. A. Genetic control of isoniazid metabolism in man. Br Med J. 1960 Aug 13;2(5197):485–491. doi: 10.1136/bmj.2.5197.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EVANS D. A., WHITE T. A. HUMAN ACETYLATION POLYMORPHISM. J Lab Clin Med. 1964 Mar;63:394–403. [PubMed] [Google Scholar]
  5. Evans D. A. An improved and simplified method of detecting the acetylator phenotype. J Med Genet. 1969 Dec;6(4):405–407. doi: 10.1136/jmg.6.4.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JENNE J. W. Deterioration of isoniazid in frozen serum samples: a confirmatory note. Am Rev Respir Dis. 1961 Jun;83:906–907. doi: 10.1164/arrd.1961.83.6.906. [DOI] [PubMed] [Google Scholar]
  7. JENNE J. W. Studies of human patterns of isoniazid metabolism using an intravenous fall-off technique with a chemical method. Am Rev Respir Dis. 1960 Jan;81:1–8. doi: 10.1164/arrd.1960.81.1.1. [DOI] [PubMed] [Google Scholar]
  8. LLOYD J., MITCHISON D. A. A VERTICAL DIFFUSION METHOD FOR THE MICROBIOLOGICAL ASSAY OF ISONIAZID. J Clin Pathol. 1964 Nov;17:622–626. doi: 10.1136/jcp.17.6.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Menon N. K. Madras study of supervised once-weekly chemotherapy. Bull Int Union Tuberc. 1968 Dec;41:316–321. [PubMed] [Google Scholar]
  10. PETERS J. H., GORDON G. R., BROWN P. THE RELATIONSHIP BETWEEN THE CAPACITIES OF HUMAN SUBJECTS TO ACETYLATE ISONIAZID, SULFANILAMIDE AND SULFAMETHAZINE. Life Sci. 1965 Jan;4:99–107. doi: 10.1016/0024-3205(65)90040-8. [DOI] [PubMed] [Google Scholar]
  11. POOLE N. F., MEYER A. E. Stability of isoniazid in aqueous solutions and plasma. Proc Soc Exp Biol Med. 1960 Aug-Sep;104:560–562. doi: 10.3181/00379727-104-25907. [DOI] [PubMed] [Google Scholar]
  12. Peters J. H., Miller K. S., Brown P. Studies on the metabolic basis for the genetically determined capacities for isoniazid inactivation in man. J Pharmacol Exp Ther. 1965 Nov;150(2):298–304. [PubMed] [Google Scholar]
  13. SELKON J. B., FOX W., GANGADHARAM P. R., RAMACHANDRAN K., RAMAKRISHNAN C. V., VELU S. Rate of inactivation of isoniazid in South Indian patients with pulmonary tuberculosis. 2. Clinical implications in the treatment of pulmonary tuberculosis with isoniazid either alone or in combination with PAS. Bull World Health Organ. 1961;25:779–792. [PMC free article] [PubMed] [Google Scholar]
  14. SUNAHARA S., URANOM, OGAWAM Genetical and geographic studies on isoniazid inactivation. Science. 1961 Nov 10;134(3489):1530–1531. doi: 10.1126/science.134.3489.1530. [DOI] [PubMed] [Google Scholar]

Articles from British Medical Journal are provided here courtesy of BMJ Publishing Group

RESOURCES