Skip to main content
PMC home page
Clinical and Diagnostic Laboratory Immunology logoLink to Clinical and Diagnostic Laboratory Immunology
. 1995 Mar;2(2):241–245. doi: 10.1128/cdli.2.2.241-245.1995

Are soluble factors relevant for polymorphonuclear leukocyte dysregulation in septicemia?

C Wenisch 1, W Graninger 1
PMCID: PMC170137  PMID: 7697538

Abstract

Polymorphonuclear leukocytes (PMNs) of twelve patients with gram-negative septicemia exhibited a decreased capacity to phagocytize Escherichia coli and generate reactive oxygen products which normalized within 7 days of treatment. Ex vivo exchange of plasma from age-, sex-, and blood-group-identical normal controls resulted in an increase of both phagocytic capacity and reactive oxygen intermediate generation in PMNs of septicemic patients and transiently reduced phagocytosis and reactive oxygen intermediate production in PMNs of normal controls. These results suggest that extrinsic factors are crucial for PMN function.

Full Text

The Full Text of this article is available as a PDF (191.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aida Y., Pabst M. J. Priming of neutrophils by lipopolysaccharide for enhanced release of superoxide. Requirement for plasma but not for tumor necrosis factor-alpha. J Immunol. 1990 Nov 1;145(9):3017–3025. [PubMed] [Google Scholar]
  2. Babior B. M. Oxidants from phagocytes: agents of defense and destruction. Blood. 1984 Nov;64(5):959–966. [PubMed] [Google Scholar]
  3. Bass D. A., Parce J. W., Dechatelet L. R., Szejda P., Seeds M. C., Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J Immunol. 1983 Apr;130(4):1910–1917. [PubMed] [Google Scholar]
  4. Bassøe C. F., Laerum O. D., Solberg C. O., Haneberg B. Phagocytosis of bacteria by human leukocytes measured by flow cytometry. Proc Soc Exp Biol Med. 1983 Nov;174(2):182–186. doi: 10.3181/00379727-174-41722. [DOI] [PubMed] [Google Scholar]
  5. Bassøe C. F., Solberg C. O. Phagocytosis of Staphylococcus aureus by human leukocytes: quantitation by a flow cytometric and a microbiological method. Acta Pathol Microbiol Immunol Scand C. 1984 Feb;92(1):43–50. doi: 10.1111/j.1699-0463.1984.tb00050.x. [DOI] [PubMed] [Google Scholar]
  6. Berkow R. L., Wang D., Larrick J. W., Dodson R. W., Howard T. H. Enhancement of neutrophil superoxide production by preincubation with recombinant human tumor necrosis factor. J Immunol. 1987 Dec 1;139(11):3783–3791. [PubMed] [Google Scholar]
  7. Bjerknes R., Bassøe C. F. Human leukocyte phagocytosis of zymosan particles measured by flow cytometry. Acta Pathol Microbiol Immunol Scand C. 1983 Oct;91(5):341–348. [PubMed] [Google Scholar]
  8. Bone R. C. Let's agree on terminology: definitions of sepsis. Crit Care Med. 1991 Jul;19(7):973–976. doi: 10.1097/00003246-199107000-00024. [DOI] [PubMed] [Google Scholar]
  9. Böhmer R. H., Trinkle L. S., Staneck J. L. Dose effects of LPS on neutrophils in a whole blood flow cytometric assay of phagocytosis and oxidative burst. Cytometry. 1992;13(5):525–531. doi: 10.1002/cyto.990130512. [DOI] [PubMed] [Google Scholar]
  10. Cannon J. G., Tompkins R. G., Gelfand J. A., Michie H. R., Stanford G. G., van der Meer J. W., Endres S., Lonnemann G., Corsetti J., Chernow B. Circulating interleukin-1 and tumor necrosis factor in septic shock and experimental endotoxin fever. J Infect Dis. 1990 Jan;161(1):79–84. doi: 10.1093/infdis/161.1.79. [DOI] [PubMed] [Google Scholar]
  11. Cantinieaux B., Hariga C., Courtoy P., Hupin J., Fondu P. Staphylococcus aureus phagocytosis. A new cytofluorometric method using FITC and paraformaldehyde. J Immunol Methods. 1989 Jul 26;121(2):203–208. doi: 10.1016/0022-1759(89)90161-0. [DOI] [PubMed] [Google Scholar]
  12. Clancy R. M., Leszczynska-Piziak J., Abramson S. B. Nitric oxide, an endothelial cell relaxation factor, inhibits neutrophil superoxide anion production via a direct action on the NADPH oxidase. J Clin Invest. 1992 Sep;90(3):1116–1121. doi: 10.1172/JCI115929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Duignan J. P., Collins P. B., Johnson A. H., Bouchier-Hayes D. The association of impaired neutrophil chemotaxis with postoperative surgical sepsis. Br J Surg. 1986 Mar;73(3):238–240. doi: 10.1002/bjs.1800730328. [DOI] [PubMed] [Google Scholar]
  14. Dunn P. A., Tyrer H. W. Quantitation of neutrophil phagocytosis, using fluorescent latex beads. Correlation of microscopy and flow cytometry. J Lab Clin Med. 1981 Sep;98(3):374–381. [PubMed] [Google Scholar]
  15. Fong Y., Moldawer L. L., Marano M., Wei H., Tatter S. B., Clarick R. H., Santhanam U., Sherris D., May L. T., Sehgal P. B. Endotoxemia elicits increased circulating beta 2-IFN/IL-6 in man. J Immunol. 1989 Apr 1;142(7):2321–2324. [PubMed] [Google Scholar]
  16. Gardinali M., Padalino P., Vesconi S., Calcagno A., Ciappellano S., Conciato L., Chiara O., Agostoni A., Nespoli A. Complement activation and polymorphonuclear neutrophil leukocyte elastase in sepsis. Correlation with severity of disease. Arch Surg. 1992 Oct;127(10):1219–1224. doi: 10.1001/archsurg.1992.01420100077014. [DOI] [PubMed] [Google Scholar]
  17. Jochum M., Duswald K. H., Neumann S., Witte J., Fritz H. Proteinases and their inhibitors in septicemia - basic concepts and clinical implications. Adv Exp Med Biol. 1984;167:391–404. doi: 10.1007/978-1-4615-9355-3_34. [DOI] [PubMed] [Google Scholar]
  18. Kowanko I. C., Ferrante A. Stimulation of neutrophil respiratory burst and lysosomal enzyme release by human interferon-gamma. Immunology. 1987 Sep;62(1):149–151. [PMC free article] [PubMed] [Google Scholar]
  19. Lopez Diez F., Nieto M. L., Fernandez-Gallardo S., Gijon M. A., Sanchez Crespo M. Occupancy of platelet receptors for platelet-activating factor in patients with septicemia. J Clin Invest. 1989 May;83(5):1733–1740. doi: 10.1172/JCI114074. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mackensen A., Galanos C., Engelhardt R. Treatment of cancer patients with endotoxin induces release of endogenous cytokines. Pathobiology. 1991;59(4):264–267. doi: 10.1159/000163659. [DOI] [PubMed] [Google Scholar]
  21. Markert M., Glass G. A., Babior B. M. Respiratory burst oxidase from human neutrophils: purification and some properties. Proc Natl Acad Sci U S A. 1985 May;82(10):3144–3148. doi: 10.1073/pnas.82.10.3144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Martich G. D., Danner R. L., Ceska M., Suffredini A. F. Detection of interleukin 8 and tumor necrosis factor in normal humans after intravenous endotoxin: the effect of antiinflammatory agents. J Exp Med. 1991 Apr 1;173(4):1021–1024. doi: 10.1084/jem.173.4.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Michie H. R., Manogue K. R., Spriggs D. R., Revhaug A., O'Dwyer S., Dinarello C. A., Cerami A., Wolff S. M., Wilmore D. W. Detection of circulating tumor necrosis factor after endotoxin administration. N Engl J Med. 1988 Jun 9;318(23):1481–1486. doi: 10.1056/NEJM198806093182301. [DOI] [PubMed] [Google Scholar]
  24. Regel G., Nerlich M. L., Dwenger A., Seidel J., Schmidt C., Sturm J. A. Phagocytic function of polymorphonuclear leukocytes and the RES in endotoxemia. J Surg Res. 1987 Jan;42(1):74–84. doi: 10.1016/0022-4804(87)90068-0. [DOI] [PubMed] [Google Scholar]
  25. Roesler J., Hecht M., Freihorst J., Lohmann-Matthes M. L., Emmendörffer A. Diagnosis of chronic granulomatous disease and of its mode of inheritance by dihydrorhodamine 123 and flow microcytofluorometry. Eur J Pediatr. 1991 Jan;150(3):161–165. doi: 10.1007/BF01963557. [DOI] [PubMed] [Google Scholar]
  26. Rothe G., Oser A., Valet G. Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes. Naturwissenschaften. 1988 Jul;75(7):354–355. doi: 10.1007/BF00368326. [DOI] [PubMed] [Google Scholar]
  27. Shalaby M. R., Aggarwal B. B., Rinderknecht E., Svedersky L. P., Finkle B. S., Palladino M. A., Jr Activation of human polymorphonuclear neutrophil functions by interferon-gamma and tumor necrosis factors. J Immunol. 1985 Sep;135(3):2069–2073. [PubMed] [Google Scholar]
  28. Simms H. H., D'Amico R. Polymorphonuclear leukocyte dysregulation during the systemic inflammatory response syndrome. Blood. 1994 Mar 1;83(5):1398–1407. [PubMed] [Google Scholar]
  29. Solomkin J. S., Cotta L. A., Brodt J. K., Hurst J. M. Regulation of neutrophil superoxide production in sepsis. Arch Surg. 1985 Jan;120(1):93–98. doi: 10.1001/archsurg.1985.01390250081013. [DOI] [PubMed] [Google Scholar]
  30. Szejda P., Parce J. W., Seeds M. S., Bass D. A. Flow cytometric quantitation of oxidative product formation by polymorphonuclear leukocytes during phagocytosis. J Immunol. 1984 Dec;133(6):3303–3307. [PubMed] [Google Scholar]
  31. Trinkle L. S., Wellhausen S. R., McLeish K. R. A simultaneous flow cytometric measurement of neutrophil phagocytosis and oxidative burst in whole blood. Diagn Clin Immunol. 1987;5(2):62–68. [PubMed] [Google Scholar]
  32. Venezio F. R., Westenfelder G. O., Phair J. P. The adherence of polymorphonuclear leukocytes in patients with sepsis. J Infect Dis. 1982 Mar;145(3):351–357. doi: 10.1093/infdis/145.3.351. [DOI] [PubMed] [Google Scholar]
  33. Vercellotti G. M., Yin H. Q., Gustafson K. S., Nelson R. D., Jacob H. S. Platelet-activating factor primes neutrophil responses to agonists: role in promoting neutrophil-mediated endothelial damage. Blood. 1988 Apr;71(4):1100–1107. [PubMed] [Google Scholar]
  34. Vespasiano M. C., Lewandoski J. R., Zimmerman J. J. Longitudinal analysis of neutrophil superoxide anion generation in patients with septic shock. Crit Care Med. 1993 May;21(5):666–672. doi: 10.1097/00003246-199305000-00008. [DOI] [PubMed] [Google Scholar]
  35. Weisbart R. H., Golde D. W., Clark S. C., Wong G. G., Gasson J. C. Human granulocyte-macrophage colony-stimulating factor is a neutrophil activator. 1985 Mar 28-Apr 3Nature. 314(6009):361–363. doi: 10.1038/314361a0. [DOI] [PubMed] [Google Scholar]
  36. Weiss S. J. Tissue destruction by neutrophils. N Engl J Med. 1989 Feb 9;320(6):365–376. doi: 10.1056/NEJM198902093200606. [DOI] [PubMed] [Google Scholar]
  37. Wewers M. D., Rinehart J. J., She Z. W., Herzyk D. J., Hummel M. M., Kinney P. A., Davis W. B. Tumor necrosis factor infusions in humans prime neutrophils for hypochlorous acid production. Am J Physiol. 1990 Oct;259(4 Pt 1):L276–L282. doi: 10.1152/ajplung.1990.259.4.L276. [DOI] [PubMed] [Google Scholar]
  38. Wilson M. E. Effects of bacterial endotoxins on neutrophil function. Rev Infect Dis. 1985 May-Jun;7(3):404–418. doi: 10.1093/clinids/7.3.404. [DOI] [PubMed] [Google Scholar]
  39. Zakhireh B., Root R. K. Development of Oxidase activity by human bone marrow granulocytes. Blood. 1979 Aug;54(2):429–439. [PubMed] [Google Scholar]
  40. Zimmerman J. J., Millard J. R., Farrin-Rusk C. Septic plasma suppresses superoxide anion synthesis by normal homologous polymorphonuclear leukocytes. Crit Care Med. 1989 Dec;17(12):1241–1246. doi: 10.1097/00003246-198912000-00001. [DOI] [PubMed] [Google Scholar]
  41. Zimmerman J. J., Ringer T. V. Inflammatory host responses in sepsis. Crit Care Clin. 1992 Jan;8(1):163–189. [PubMed] [Google Scholar]
  42. Zimmerman J. J., Shelhamer J. H., Parrillo J. E. Quantitative analysis of polymorphonuclear leukocyte superoxide anion generation in critically ill children. Crit Care Med. 1985 Mar;13(3):143–150. doi: 10.1097/00003246-198503000-00001. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Diagnostic Laboratory Immunology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES