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. 1995 May;2(3):377–380. doi: 10.1128/cdli.2.3.377-380.1995

Cell response to a salt-extractable and sonicated Brucella melitensis 16M antigen in human brucellosis.

M C Moreno-Lafont 1, A López-Merino 1, R López-Santiago 1
PMCID: PMC170163  PMID: 7664186

Abstract

We compared the immunological responses of leukocytes taken from healthy negative controls, laboratory workers immunized with the phenol-insoluble French vaccine against brucellosis, patients acutely ill with brucellosis, and patients chronically infected with Brucella melitensis. A salt-extractable antigen (protein-rich but with traces of lipopolysaccharide) and a sonicated suspension from B. melitensis 16M were used as antigens for in vitro lymphocyte proliferation test. Quantitation of T cells showed that the ratio of CD4+/CD8+ cells decreased as the condition of the patient deteriorated. An assay to quantitate the cell-mediated immunity showed that the activities of mononuclear cells stimulated with concanavalin A increased as the disease progressed as well.

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Selected References

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