Abstract
Bartonella henselae, the major causative agent of cat scratch disease, was cocultivated with Vero cells on chamber slides and visualized by indirect immunofluorescence by using a patient serum containing specific antibodies. Confocal microscopy localized the granular B. henselae-specific fluorescence mainly around the nuclei of Vero cells. By transmission electron microscopy, these granules were identified as clusters of multiple intracellular organisms. Fixed slides with the monolayers of Vero cells with intracellular B. henselae were used for an indirect fluorescent-antibody test to investigate the seroprevalence of specific immunoglobulin G in 100 serum samples from blood donors. Seventy-four serum samples were negative; 19, 3, and 4 were positive at dilutions of 1:64, 1:128, and 1:256, respectively. In our population, a serum titer of 1:256 or greater should stimulate further investigations. Moreover, elucidation of the mechanism by which B. henselae enters the cells may help to understand the pathogenesis of cat scratch disease.
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- Anderson B., Sims K., Regnery R., Robinson L., Schmidt M. J., Goral S., Hager C., Edwards K. Detection of Rochalimaea henselae DNA in specimens from cat scratch disease patients by PCR. J Clin Microbiol. 1994 Apr;32(4):942–948. doi: 10.1128/jcm.32.4.942-948.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Birkness K. A., George V. G., White E. H., Stephens D. S., Quinn F. D. Intracellular growth of Afipia felis, a putative etiologic agent of cat scratch disease. Infect Immun. 1992 Jun;60(6):2281–2287. doi: 10.1128/iai.60.6.2281-2287.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dolan M. J., Wong M. T., Regnery R. L., Jorgensen J. H., Garcia M., Peters J., Drehner D. Syndrome of Rochalimaea henselae adenitis suggesting cat scratch disease. Ann Intern Med. 1993 Mar 1;118(5):331–336. doi: 10.7326/0003-4819-118-5-199303010-00002. [DOI] [PubMed] [Google Scholar]
- English C. K., Wear D. J., Margileth A. M., Lissner C. R., Walsh G. P. Cat-scratch disease. Isolation and culture of the bacterial agent. JAMA. 1988 Mar 4;259(9):1347–1352. doi: 10.1001/jama.259.9.1347. [DOI] [PubMed] [Google Scholar]
- Fumarola D., Pece S., Fumarulo R., Petruzzelli R., Greco B., Giuliani G., Maffione A. B., Jirillo E. Downregulation of human polymorphonuclear cell activities exerted by microorganisms belonging to the alpha-2 subgroup of Proteobacteria (Afipia felis and Rochalimaea henselae). Immunopharmacol Immunotoxicol. 1994 Aug;16(3):449–461. doi: 10.3109/08923979409007104. [DOI] [PubMed] [Google Scholar]
- Koehler J. E., Glaser C. A., Tappero J. W. Rochalimaea henselae infection. A new zoonosis with the domestic cat as reservoir. JAMA. 1994 Feb 16;271(7):531–535. doi: 10.1001/jama.271.7.531. [DOI] [PubMed] [Google Scholar]
- Osborne B. M., Butler J. J., Mackay B. Ultrastructural observations in cat scratch disease. Am J Clin Pathol. 1987 Jun;87(6):739–744. doi: 10.1093/ajcp/87.6.739. [DOI] [PubMed] [Google Scholar]
- Regnery R. L., Olson J. G., Perkins B. A., Bibb W. Serological response to "Rochalimaea henselae" antigen in suspected cat-scratch disease. Lancet. 1992 Jun 13;339(8807):1443–1445. doi: 10.1016/0140-6736(92)92032-b. [DOI] [PubMed] [Google Scholar]
- Waldvogel K., Regnery R. L., Anderson B. E., Caduff R., Caduff J., Nadal D. Disseminated cat-scratch disease: detection of Rochalimaea henselae in affected tissue. Eur J Pediatr. 1994 Jan;153(1):23–27. [PubMed] [Google Scholar]
- Wear D. J., Margileth A. M., Hadfield T. L., Fischer G. W., Schlagel C. J., King F. M. Cat scratch disease: a bacterial infection. Science. 1983 Sep 30;221(4618):1403–1405. doi: 10.1126/science.6612349. [DOI] [PubMed] [Google Scholar]