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. 1971 Jan;41(1):8–18. doi: 10.1111/j.1476-5381.1971.tb09930.x

Preparation and assay of urogastrone, an inhibitor of gastric acid secretion

A J Lawrence, H O Schild, G M Smith
PMCID: PMC1702741  PMID: 5547765

Abstract

1. Urogastrone, an acid gastric secretion inhibitor extracted from human urine, was assayed in the anaesthetized rat by circulating through its stomach a succinic-propionic buffer and recording electrometrically the secretion of acid in response to carbachol, and inhibition of carbachol secretion by urogastrone.

2. Quantitative assay procedures are described requiring the administration of only one or two doses of urogastrone to each rat.

3. An extraction procedure for urogastrone is described based on the use of zeocarb, a cationic polystyrene resin.

4. The most purified samples contained 40-80 times the amount/milligram which was just detectable by the assay method.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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