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. 1971 Jan;41(1):65–75. doi: 10.1111/j.1476-5381.1971.tb09936.x

Effects of Na+ and K+ on the uptake of metaraminol by rabbit ventricular slices

D M Paton
PMCID: PMC1702743  PMID: 4251250

Abstract

1. The ionic dependence of [3H]-metaraminol transport by rabbit ventricular slices was studied.

2. Transport was Na+ dependent. Choline, Li+, K+, Rb+ or Cs+ could not be substituted for Na+.

3. Transport was K+ dependent. Rb+ and Cs+, but not Li, could be substituted for K+, their relative potencies being K+ = or >Rb+>Cs+>>Li+. Higher concentrations of K+, Rb+, Cs+ and Li inhibited [3H]-metaraminol transport.

4. The inhibitory effects on transport of either a marked reduction in the concentration of Na+ or of omission of K+ were rapidly reversible on exposure of slices to Krebs solution.

5. The inhibitory effect of ouabain on [3H]-metaraminol transport was markedly time dependent, being significantly increased by preincubation with ouabain. The inhibitory action of ouabain was not affected, however, by the Na+ concentration present during preincubation.

6. An inwardly directed Na+ gradient did not increase [3H]-metaraminol transport in slices in which Na+ pumping had been prevented by 10-5M ouabain, 4° C, omission of K+ or by metabolic inhibition.

7. These findings provide additional evidence that the Na+ and K+ activated adenosine triphosphatase may participate in the transport of metaraminol and related amines. In the absence of Na+ pumping, induced Na+ gradients were unable to produce transport of amine as would be predicted by the co-transport model proposed by Bogdanski & Brodie (1969).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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