Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1970 Oct;40(2):326–333. doi: 10.1111/j.1476-5381.1970.tb09925.x

Subcellular localization of renin and kininogenase in the rat kidney

K Nustad, I Rubin
PMCID: PMC1702885  PMID: 4321758

Abstract

1. The distribution of enzymatic activities was determined in subcellular fractions of rat kidney cortex homogenates after various homogenization procedures. The specific activities of kininogenase (KGA), BAEE esterase (pH 8·5), alkaline phosphatase and glucose-6-phosphatase were, on average, 3·4 times higher in the microsomal fraction than in the whole homogenate. The total amount of these activities in the microsomal fraction after gentle, ordinary and forced homogenization were about 15, 40 and 65% of total recovered activities, respectively. These results confirmed the localization of KGA in the microsomal fraction.

2. Renin activity was primarily recovered in the heavy mitochondrial fraction. When the force of the homogenization was increased some renin activity was shifted to the soluble fraction.

3. When a mixture of renin and purified urinary KGA was given intravenously to an anaesthetized rat, a hypotensive response due to the KGA was followed by a hypertensive renin response. Over a certain range of concentrations KGA and renin could be measured simultaneously. In fractions of kidney homogenates, however, KGA activity was too low to be measured by this method.

Full text

PDF
326

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEAUFAY H., BENDALL D. S., BAUDHUIN P., DE DUVE C. Tissue fractionation studies. 12. Intracellular distribution of some dehydrogenases, alkaline deoxyribonuclease and iron in rat-liver tissue. Biochem J. 1959 Dec;73:623–628. doi: 10.1042/bj0730623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BONTING S. L., POLLAK V. E., MUEHRCKE R. C., KARK R. M. Quantitative histochemistry of the nephron. II. Alkaline phosphatase activity in man and other species. J Clin Invest. 1960 Sep;39:1372–1380. doi: 10.1172/JCI104156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COOK W. F., PICKERING G. W. The location of renin in the kidney. Biochem Pharmacol. 1962 Aug;9:165–171. doi: 10.1016/0006-2952(62)90024-2. [DOI] [PubMed] [Google Scholar]
  4. Carvalho I. F., Diniz C. R. Kinin-forming enzyme (kininogenin) in homogenates of rat kidney. Biochim Biophys Acta. 1966 Oct 17;128(1):136–148. doi: 10.1016/0926-6593(66)90150-0. [DOI] [PubMed] [Google Scholar]
  5. Chiang T. S., Erdös E. G., Miwa I., Tague L. L., Coalson J. J. Isolation from a salivary gland of granules containing renin and kallikrein. Circ Res. 1968 Oct;23(4):507–517. doi: 10.1161/01.res.23.4.507. [DOI] [PubMed] [Google Scholar]
  6. DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DE DUVE C., WATTIAUX R. Tissue fractionation studies. VII. Release of bound hydrolases by means of triton X-100. Biochem J. 1956 Aug;63(4):606–608. doi: 10.1042/bj0630606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DENGLER H., REICHEL G. [Studies on the intracellular localization of renin and hypertensinase activity]. Experientia. 1960 Jan 15;16:36–38. doi: 10.1007/BF02201791. [DOI] [PubMed] [Google Scholar]
  9. Fisher E. R. Lysosomal nature of juxtaglomerular granules. Science. 1966 Jun 24;152(3730):1752–1753. doi: 10.1126/science.152.3730.1752. [DOI] [PubMed] [Google Scholar]
  10. Gomba S., Soltész B. M. Histochemistry of lysosomal enzymes in juxtaglomerular cells. Experientia. 1969 May 15;25(5):513–513. doi: 10.1007/BF01900791. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Matsunaga M., Kira J., Saito N., Ogino K., Takayasu M. Acid angiotensinase, renin, and acid adenosine triphosphatase in rat kidney lysosomes. Jpn Circ J. 1968 Feb;32(2):137–143. doi: 10.1253/jcj.32.137. [DOI] [PubMed] [Google Scholar]
  13. Ng K. K., Vane J. R. Fate of angiotensin I in the circulation. Nature. 1968 Apr 13;218(5137):144–150. doi: 10.1038/218144a0. [DOI] [PubMed] [Google Scholar]
  14. Nustad K. Localization of kininogenase in the rat kidney. Br J Pharmacol. 1970 May;39(1):87–98. doi: 10.1111/j.1476-5381.1970.tb09558.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nustad K. The relationship between kidney and urinary kininogenase. Br J Pharmacol. 1970 May;39(1):73–86. doi: 10.1111/j.1476-5381.1970.tb09557.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ogino K., Matsunaga M., Saito N., Kira J., Takayasu M. Renin and acid adenosine triphosphatase as lysosomal enzymes. Jpn Circ J. 1967 Jan;31(1):1–8. doi: 10.1253/jcj.31.1. [DOI] [PubMed] [Google Scholar]
  17. PAGE I. H., BUMPUS F. M. Angiotensin. Physiol Rev. 1961 Apr;41:331–390. doi: 10.1152/physrev.1961.41.2.331. [DOI] [PubMed] [Google Scholar]
  18. Pickens P. T., Bumpus F. M., Lloyd A. M., Smeby R. R., Page I. H. Measurement of renin activity in human plasma. Circ Res. 1965 Nov;17(5):438–448. doi: 10.1161/01.res.17.5.438. [DOI] [PubMed] [Google Scholar]
  19. ROBERTS P. S. Measurement of the rate of plasmin action on synthetic substrates. J Biol Chem. 1958 May;232(1):285–291. [PubMed] [Google Scholar]
  20. ROBERTS P. S. The esterase activities of human plasmin during purification and subsequent activation by streptokinase or glycerol. J Biol Chem. 1960 Aug;235:2262–2266. [PubMed] [Google Scholar]
  21. Robertson A. L., Jr, Smeby R. R., Bumpus F. M., Page I. H. Production of renin by human juxtaglomercular cells in vitro. Circ Res. 1966 Jun;18(1 Suppl 1):131–142. doi: 10.1161/01.res.18.s6.i-131. [DOI] [PubMed] [Google Scholar]
  22. SHIBKO S., TAPPEL A. L. RAT-KIDNEY LYSOSOMES: ISOLATION AND PROPERTIES. Biochem J. 1965 Jun;95:731–741. doi: 10.1042/bj0950731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SKEGGS L. T., Jr, KAHN J. R., MARSH W. H. A method of assaying small amounts of hypertensin. Lab Invest. 1953 Mar-Apr;2(2):109–114. [PubMed] [Google Scholar]
  24. Schachter M. Kallikreins and kinins. Physiol Rev. 1969 Jul;49(3):509–547. doi: 10.1152/physrev.1969.49.3.509. [DOI] [PubMed] [Google Scholar]
  25. WERLE E., VOGEL R. [On the excretion of kallikrein in urine after experimental kidney injury]. Arch Int Pharmacodyn Ther. 1960 Jun 1;126:171–186. [PubMed] [Google Scholar]
  26. Wattiaux-De Coninck S., Rutgeerts M. J., Wattiaux R. Lysosomes in rat-kidney tissue. Biochim Biophys Acta. 1965 Sep 20;105(3):446–459. doi: 10.1016/s0926-6593(65)80230-2. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES