Abstract
1. An oxytocic substance has been isolated from ox hypothalamus by successive gel filtration on Sephadex G-25 and Sephadex G-50, and its pharmacology has been examined on three smooth muscle preparations.
2. The substance has the same order of potency on rat uterus, guinea-pig ileum, and hen rectal caecum.
3. The action of the substance on rat uterus was not abolished by thioglycollate.
4. Atropine (1.0 μg/ml.), phenoxybenzamine (0.1 μg/ml.) and mepyramine (1.0 μg/ml.) did not block the smooth muscle action of the substance.
5. Drug action, relative potency, and log dose-response relationships distinguish the substance from 5-hydroxytryptamine, acetylcholine, oxytocin, vasopressin, angiotensin amide, bradykinin, and purified preparations of substance P.
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Selected References
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- BISSET G. W., LEWIS G. P. A spectrum of pharmacological activity in some biologically active peptides. Br J Pharmacol Chemother. 1962 Aug;19:168–182. [PMC free article] [PubMed] [Google Scholar]
- CLEUGH J., GADDUM J. H., HOLTON P., LEACH E. Assay of substance P on the fowl rectal caecum. Br J Pharmacol Chemother. 1961 Aug;17:144–158. doi: 10.1111/j.1476-5381.1961.tb01115.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CLEUGH J., GADDUM J. H., MITCHELL A. A., SMITH M. W., WHITTAKER V. P. SUBSTANCE P IN BRAIN EXTRACTS. J Physiol. 1964 Jan;170:69–85. doi: 10.1113/jphysiol.1964.sp007314. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAWKER R. W., BLACKSHAW J. K., ROBERTS V. S., GOODRICKE B. Oxytocic activity of kitten hypothalamic extracts. Med J Aust. 1962 Oct 20;49(2):621–624. [PubMed] [Google Scholar]
- LEDERIS K. Vasopressin and oxytocin in the mammalian hypothalamus. Gen Comp Endocrinol. 1961 Apr;1:80–89. doi: 10.1016/0016-6480(61)90027-2. [DOI] [PubMed] [Google Scholar]
- MUNSICK R. A. Effect of magnesium ion on the response of the rat uterus to neurohypophysial hormones and analogues. Endocrinology. 1960 Mar;6:451–457. doi: 10.1210/endo-66-3-451. [DOI] [PubMed] [Google Scholar]
- North W. G., Hawker R. W., Zerner B. Isolation of new oxytocic substance from ox hypothalamus. Biochim Biophys Acta. 1968 Jun 26;160(2):286–288. doi: 10.1016/0005-2795(68)90107-4. [DOI] [PubMed] [Google Scholar]
- SAWYER W. H. Neurophypophysial hormones. Pharmacol Rev. 1961 Jun;13:225–277. [PubMed] [Google Scholar]
- Valtin H., Sawyer W. H., Sokol H. W. Neurohypophysial principles in rats homozygous and heterozygous for hypothalamic diabetes insipidus (Brattleboro strain). Endocrinology. 1965 Oct;77(4):701–706. doi: 10.1210/endo-77-4-701. [DOI] [PubMed] [Google Scholar]
- ZETLER G. New pharmacologically active polypeptides present in impure preparations of substance P. Ann N Y Acad Sci. 1963 Feb 4;104:416–436. doi: 10.1111/j.1749-6632.1963.tb17686.x. [DOI] [PubMed] [Google Scholar]