Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1976 Oct;32(4):617–622. doi: 10.1128/aem.32.4.617-622.1976

Morphological characterization of small cells resulting from nutrient starvation of a psychrophilic marine vibrio.

J A Novitsky, R Y Morita
PMCID: PMC170316  PMID: 984833

Abstract

Upon starvation, Ant-300, a psychrophilic marine vibrio, was observed to decrease in size and change in shape from a rod to a coccus. After 3 weeks of starvation 50% of the starved population was able to pass through a filter with a pore size of 0.4 mum. Electron microscopy of thin sections of the small cells revealed normal cell structure except for an enlarged periplasmic space. When inoculated into a fresh medium, starved cells growth without a significant lag and regained "normal" size and shape within 48 h.

Full text

PDF
617

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson J. I., Heffernan W. P. Isolation and characterization of filterable marine bacteria. J Bacteriol. 1965 Dec;90(6):1713–1718. doi: 10.1128/jb.90.6.1713-1718.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bae H. C., Cota-Robles E. H., Casida L. E. Microflora of soil as viewed by transmission electron microscopy. Appl Microbiol. 1972 Mar;23(3):637–648. doi: 10.1128/am.23.3.637-648.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baker D. A., Park R. W. Changes in morphology and cell wall structure that occur during growth of Vibrio sp. NCTC4716 in batch culture. J Gen Microbiol. 1975 Jan;86(1):12–28. doi: 10.1099/00221287-86-1-12. [DOI] [PubMed] [Google Scholar]
  5. Boylen C. W., Pate J. L. Fine structure of Arthrobacter crystallopoietes during long-term starvation of rod and spherical stage cells. Can J Microbiol. 1973 Jan;19(1):1–5. doi: 10.1139/m73-001. [DOI] [PubMed] [Google Scholar]
  6. CASIDA L. E., Jr ABUNDANT MICROORGANISM IN SOIL. Appl Microbiol. 1965 May;13:327–334. doi: 10.1128/am.13.3.327-334.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Casida L. E., Jr Microorganisms in unamended soil as observed by various forms of microscopy and staining. Appl Microbiol. 1971 Jun;21(6):1040–1045. doi: 10.1128/am.21.6.1040-1045.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. ENSIGN J. C., WOLFE R. S. NUTRITIONAL CONTROL OF MORPHOGENESIS IN ARTHROBACTER CRYSTALLOPIETES. J Bacteriol. 1964 Apr;87:924–932. doi: 10.1128/jb.87.4.924-932.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Felter R. A., Colwell R. R., Chapman G. B. Morphology and round body fermation in Vibrio marinus. J Bacteriol. 1969 Jul;99(1):326–335. doi: 10.1128/jb.99.1.326-335.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MOLLENHAUER H. H. PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY. Stain Technol. 1964 Mar;39:111–114. [PubMed] [Google Scholar]
  11. OPPENHEIMER C. H. The membrane filter in marine microbiology. J Bacteriol. 1952 Dec;64(6):783–786. doi: 10.1128/jb.64.6.783-786.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TUCKETT J. D., MOORE W. E. Production of filterable particles by Cellvibrio gilvus. J Bacteriol. 1959 Feb;77(2):227–229. doi: 10.1128/jb.77.2.227-229.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES