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. 1976 Oct;32(4):635–637. doi: 10.1128/aem.32.4.635-637.1976

Isolation of strains of the cellular slime mold Dictyostelium discoideum capable of growing after a single passage in axenic medium.

K L Williams
PMCID: PMC170319  PMID: 988786

Abstract

Wild-type strains of the cellular slime mold Dictyostelium discoideum grow on bacteria. Axenic strains of D. discoideum capable of growing in a simple salts-yeast extract-proteose peptone-glucose medium have been isolated from wild-type strains fo both mating types after a single passage in this medium. This result is consistent with the theory that the axenic phenotype has a simple genetic basis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Erdos G. W., Raper K. B., Vogen L. K. Mating Types and Macrocyst Formation in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1828–1830. doi: 10.1073/pnas.70.6.1828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Loomis W. F., Jr Sensitivity of Dictyostelium discoideum to nucleic acid analogues. Exp Cell Res. 1971 Feb;64(2):484–486. doi: 10.1016/0014-4827(71)90107-8. [DOI] [PubMed] [Google Scholar]
  3. Sussman R., Sussman M. Cultivation of Dictyostelium discoideum in axenic medium. Biochem Biophys Res Commun. 1967 Oct 11;29(1):53–55. doi: 10.1016/0006-291x(67)90539-6. [DOI] [PubMed] [Google Scholar]
  4. Watts D. J., Ashworth J. M. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970 Sep;119(2):171–174. doi: 10.1042/bj1190171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Williams K. L., Kessin R. H., Newell P. C. Genetics of growth in axenic medium of the cellular slime mould Dictyostelium discoideum. Nature. 1974 Jan 18;247(5437):142–143. doi: 10.1038/247142a0. [DOI] [PubMed] [Google Scholar]
  6. Williams K. L., Kessin R. H., Newell P. C. Parasexual genetics in Dictyostelium discoideum: mitotic analysis of acriflavin resistance and growth in axenic medium. J Gen Microbiol. 1974 Sep;84(1):59–69. doi: 10.1099/00221287-84-1-59. [DOI] [PubMed] [Google Scholar]

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