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. 1969 Mar;35(3):543–557. doi: 10.1111/j.1476-5381.1969.tb08295.x

Partial purification of the anti-inflammatory factor(s) in inflammatory exudate

M E J Billingham, B V Robinson, J M Robson
PMCID: PMC1703364  PMID: 5809743

Abstract

1. The carrageenin foot test was established as a sensitive and reliable assay procedure for determining the anti-inflammatory activity of inflammatory exudates.

2. Incubation alone at a temperature above 70° C or with pronase at 37° C destroyed the anti-inflammatory activity of exudate.

3. The anti-inflammatory component of exudate was partially precipitated by 50% ammonium sulphate.

4. A partial purification process was devised using Sephadex G-150 gel filtration and DEAE and CM cellulose ion exchange chromatography to obtain at least a 24 fold purification.

5. Measurements of 11-hydroxycorticosteroid levels indicated that steroids were not involved in the mechanism by which the exudate produced its anti-inflammatory effects.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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