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. 1969 Mar;35(3):406–417. doi: 10.1111/j.1476-5381.1969.tb08282.x

3H-Nicotine in cat superior cervical and nodose ganglia after close-arterial injection in vivo

D A Brown, P C Hoffmann, L J Roth
PMCID: PMC1703366  PMID: 5809733

Abstract

1. Concentrations of 3H-nicotine in the superior cervical and nodose ganglia of anaesthetized cats were measured after close-arterial injection.

2. Shortly after injection there was a higher concentration of 3H-nicotine in the superior cervical ganglion than in the nodose ganglion. Mean concentration ratios, superior cervical ganglion/nodose ganglion (S/N ratios) were: 2 min after injection, 1.60 ± 0.19; 4 min, 1.21 ± 0.19; 8 min, 0.92 ± 0.05. These ratios were independent of the dose of nicotine over the range 4 to 200 μg in 0.2 ml.

3. There was no comparable difference in the concentrations of injected 14C-inulin or 3H2O in the two ganglia, or in total water content.

4. Procedures which reduced the pharmacological action of nicotine (pre-treatment with hexamethonium, admixture of 14C-inulin) tended to reduce the S/N ratio for nicotine.

5. Autoradiographs showed that nicotine entered the neurones of both superior cervical and nodose ganglia.

6. It was concluded that the higher concentration of nicotine in the superior cervical ganglion was probably related to its selective pharmacological action at this site, and may have been due to a greater intracellular retention of nicotine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. APPELGREN L. E., HANSSON E., SCHMITERLOEW C. G. LOCALIZATION OF RADIOACTIVITY IN THE SUPERIOR CERVICAL GANGLION OF CATS FOLLOWING INJECTION OF C14-LABELLED NICOTINE. Acta Physiol Scand. 1963 Dec;59:330–336. doi: 10.1111/j.1748-1716.1963.tb02748.x. [DOI] [PubMed] [Google Scholar]
  2. CREESE R., TAYLOR D. B., TILTON B. The influence of curare on the uptake and release of a neuromuscular blocking agent labeled with radioactive iodine. J Pharmacol Exp Ther. 1963 Jan;139:8–17. [PubMed] [Google Scholar]
  3. Creese R., Maclagan J. Autoradiography of decamethonium in rat muscle. Nature. 1967 Aug 26;215(5104):988–989. doi: 10.1038/215988a0. [DOI] [PubMed] [Google Scholar]
  4. HANSSON E., HOFFMANN P. C., SCHMITERLOEW C. G. METABOLISM OF NICOTINE IN MOUSE TISSUE SLICES. Acta Physiol Scand. 1964 Aug;61:380–392. [PubMed] [Google Scholar]
  5. PATON W. D., RANG H. P. THE UPTAKE OF ATROPINE AND RELATED DRUGS BY INTESTINAL SMOOTH MUSCLE OF THE GUINEA-PIG IN RELATION TO ACETYLCHOLINE RECEPTORS. Proc R Soc Lond B Biol Sci. 1965 Aug 24;163:1–44. doi: 10.1098/rspb.1965.0058. [DOI] [PubMed] [Google Scholar]
  6. Stumpf W. E., Roth L. J. Freeze-drying of small tissue samples and thin frozen sections below -60 degrees C. A simple method of cryosorption pumping. J Histochem Cytochem. 1967 Apr;15(4):243–251. doi: 10.1177/15.4.243. [DOI] [PubMed] [Google Scholar]
  7. Stumpf W. E., Roth L. J. High resolution autoradiography with dry mounted, freeze-dried frozen sections. Comparative study of six methods using two diffusible compounds 3H-estradiol and 3H-mesobilirubinogen. J Histochem Cytochem. 1966 Mar;14(3):274–287. doi: 10.1177/14.3.274. [DOI] [PubMed] [Google Scholar]
  8. WASER P. G. The cholinergic receptor. J Pharm Pharmacol. 1960 Oct;12:577–594. doi: 10.1111/j.2042-7158.1960.tb12714.x. [DOI] [PubMed] [Google Scholar]

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