Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1968 Sep;34(1):148–158.

The assay of anti-pyretic drugs in mice, using intracerebral injection of pyretogenins

C H Cashin, Christine E Heading
PMCID: PMC1703404  PMID: 5676003

Abstract

1. A simple, cheap, and reliable method is described for the screening of new compounds for anti-pyretic activity. It involves the intracerebral injection of a suitable pyretogenin in conscious mice.

2. The pyretic response is related to the logarithm of the dose in a substantially linear manner when “E” Pyrogen is used.

3. A selection of established anti-pyretic drugs were effective in oral doses approaching those used clinically, which shows that the method is unusually sensitive.

4. The response to intracerebral pyretogenin is not unduly influenced by drugs known to cause hypothermia.

5. It is suggested that “E” Pyrogen injected intracerebrally has a direct effect on the central nervous system, rather than an indirect action through release of endogenous pyrogen.

Full text

PDF
148

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS S. S., CLIFFE E. E., LESSEL B., NICHOLSON J. S. SOME BIOLOGICAL PROPERTIES OF 'IBUFENAC', A NEW ANTI-RHEUMATIC DRUG. Nature. 1963 Oct 19;200:271–272. doi: 10.1038/200271b0. [DOI] [PubMed] [Google Scholar]
  2. Atkins E., Bodel P., Francis L. Release of an endogenous pyrogen in vitro from rabbit mononuclear cells. J Exp Med. 1967 Aug 1;126(2):357–384. doi: 10.1084/jem.126.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOUCHARD E. F., LEYDEN A. F., POMERANTZ E. Pharmaceutical aspects of reserpine. J Am Pharm Assoc Am Pharm Assoc. 1956 Dec;45(12):771–775. [PubMed] [Google Scholar]
  4. BOXILL G. C., NASH C. B., WHEELER A. G. Comparative pharmacological and toxicological evaluation of N-acetyl-p-aminophenol, salicylamide, and acetylsalicylic acid. J Am Pharm Assoc Am Pharm Assoc. 1958 Jul;47(7):479–487. doi: 10.1002/jps.3030470706. [DOI] [PubMed] [Google Scholar]
  5. Brittain R. T. The intracerebral effects of noradrenaline and its modification by drugs in the mouse. J Pharm Pharmacol. 1966 Sep;18(9):621–623. doi: 10.1111/j.2042-7158.1966.tb07944.x. [DOI] [PubMed] [Google Scholar]
  6. Cooper K. E., Cranston W. I., Honour A. J. Observations on the site & mode of action of pyrogens in the rabbit brain. J Physiol. 1967 Jul;191(2):325–337. doi: 10.1113/jphysiol.1967.sp008253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HALEY T. J., MCCORMICK W. G. Pharmacological effects produced by intracerebral injection of drugs in the conscious mouse. Br J Pharmacol Chemother. 1957 Mar;12(1):12–15. doi: 10.1111/j.1476-5381.1957.tb01354.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hahn H. H., Char D. C., Postel W. B., Wood W. B., Jr Studies on the pathogenesis of fever. XV. The production of endogenous pyrogen by peritoneal macrophages. J Exp Med. 1967 Aug 1;126(2):385–394. doi: 10.1084/jem.126.2.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. ROSENTHAL M. W., MARKLEY J. F., SCHUBERT J. Comparison of antipyretic activity of salicylic and aurintricarboxylic acids. Proc Soc Exp Biol Med. 1963 Mar;112:565–567. doi: 10.3181/00379727-112-28106. [DOI] [PubMed] [Google Scholar]
  10. WINDER C. V., WAX J., SCOTTI L., SCHERRER R. A., JONES E. M., SHORT F. W. Anti-inflammatory, antipyretic and antinociceptive properties of N-(2,3-xylyl)anthranilic acid (mefenamic acid). J Pharmacol Exp Ther. 1962 Dec;138:405–413. [PubMed] [Google Scholar]
  11. WINDER C. V., WAX J., SERRANO B., JONES E. M., McPHEE M. L. Anti-inflammatory and antipyretic properties of N-(alpha,alpha,alpha-trifluoro-m-tolyl) anthranilic acid (CI-440; flufenamic acid). Arthritis Rheum. 1963 Feb;6:36–47. doi: 10.1002/art.1780060105. [DOI] [PubMed] [Google Scholar]
  12. WINDER C. V., WAX J., WELFORD M. ANTI-INFLAMMATORY AND ANTIPYRETIC PROPERTIES OF N-(2,6-DICHLORO-M-TOLYL)ANTHRANILIC ACID (CI-583). J Pharmacol Exp Ther. 1965 Jun;148:422–429. [PubMed] [Google Scholar]
  13. WINTER C. A., NUSS G. W. Pyretogenic effects of bacterial lipopolysaccharide and the assay of antipyretic drugs in rats. Toxicol Appl Pharmacol. 1963 Mar;5:247–256. doi: 10.1016/0041-008x(63)90047-4. [DOI] [PubMed] [Google Scholar]
  14. WINTER C. A., RISLEY E. A., NUSS G. W. ANTI-INFLAMMATORY AND ANTIPYRETIC ACTIVITIES OF INDOMETHACIN, 1-(P-CHLOROBENZOYL)-5-METHOXY-2-METHYLINDOLE-3-ACETIC ACID. J Pharmacol Exp Ther. 1963 Sep;141:369–376. [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES