Abstract
1. In isolated 5-hydroxytryptamine (5-HT) organelles of rabbit platelets, the concentrations of 5-HT, histamine and adenosine-triphosphate (ATP) respectively are about 200 times higher than in intact platelets. Organelles incubated in plasma at 37° C gradually lose endogenous 5-HT, histamine and ATP and take up 14C-5-HT against a considerable concentration gradient. Liberation and uptake of 5-HT markedly decrease with dimishing incubation temperature.
2. Exposure to reserpine in vitro strongly counteracts the uptake of 14C-5-HT by isolated organelles, whereas the 14C-5-HT uptake of intact isolated platelets is less affected by the drug. 5-HT organelles of platelets from reserpinized rabbits also take up very little 14C-5-HT.
3. Imipramine inhibits the uptake of 14C-5-HT in isolated organelles less markedly than in isolated platelets.
4. It is concluded that in the organelles 5-HT and possibly histamine may be associated with ATP. Reserpine probably impairs the uptake of 5-HT at the level of the organelles (possibly by interfering with the association 5-HT/ATP), whereas imipramine seems to act preferentially on the cell membrane.
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Selected References
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- BARTHOLINI G., PLETSCHER A., GEY K. F. Diminution of 5-hydroxytryptamine in thrombocytes in vitro by chlorpromazine and related compounds. Experientia. 1961 Dec 15;17:541–542. doi: 10.1007/BF02156407. [DOI] [PubMed] [Google Scholar]
- BOGDANSKI D. F., PLETSCHER A., BRODIE B. B., UNDENFRIEND S. Identification and assay of serotonin in brain. J Pharmacol Exp Ther. 1956 May;117(1):82–88. [PubMed] [Google Scholar]
- Bak I. J., Hassler R., May B., Westermann E. Morphological and biochemical studies on the storage of serotonin and histamine in blood platelets of the rabbit. Life Sci. 1967 Jun 1;6(11):1133–1146. doi: 10.1016/0024-3205(67)90195-6. [DOI] [PubMed] [Google Scholar]
- Carlsson A. Modification of sympathetic function. Pharmacological depletion of catecholamine stores. Pharmacol Rev. 1966 Mar;18(1):541–549. [PubMed] [Google Scholar]
- Da Prada M., Pletscher A., Bartholini G. Temperature sensitivity of the passive outflow of 5-hydroxytryptamine from blood platelets. Life Sci. 1965 Sep;4(18):1773–1778. doi: 10.1016/0024-3205(65)90239-0. [DOI] [PubMed] [Google Scholar]
- Da Prada M., Pletscher A., Tranzer J. P., Knuchel H. Subcellular localization of 5-hydroxytryptamine and histamine in blood platelets. Nature. 1967 Dec 30;216(5122):1315–1317. doi: 10.1038/2161315a0. [DOI] [PubMed] [Google Scholar]
- Holmsen H., Holmsen I., Bernhardsen A. Microdetermination of adenosine diphosphate and adenosine triphosphate in plasma with firefly luciferase system. Anal Biochem. 1966 Dec;17(3):456–473. doi: 10.1016/0003-2697(66)90181-3. [DOI] [PubMed] [Google Scholar]
- Huff J. A., Davis V. E., Brown H. A simplified technique for the estimation of histamine in blood and urine. J Lab Clin Med. 1966 Jun;67(6):1044–1047. [PubMed] [Google Scholar]
- Pletscher A., Burkard W. P., Tranzer J. P., Gey K. F. Two sites of 5-hydroxytryptamine uptake in blood platelets. Life Sci. 1967 Feb 1;6(3):273–280. doi: 10.1016/0024-3205(67)90156-7. [DOI] [PubMed] [Google Scholar]
- Pletscher A. Metabolism, transfer and storage of 5-hydroxytryptamine in blood platelets. Br J Pharmacol Chemother. 1968 Jan;32(1):1–16. doi: 10.1111/j.1476-5381.1968.tb00423.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tranzer J. P., Da Prada M., Pletscher A. Electron microscopic study of the storage site of 5-hydroxytryptamine in blood platelets. Adv Pharmacol. 1968;6(Pt A):125–128. doi: 10.1016/s1054-3589(08)61164-3. [DOI] [PubMed] [Google Scholar]