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. 1996 Jul;3(4):387–391. doi: 10.1128/cdli.3.4.387-391.1996

Specific serodiagnosis of human leishmaniasis with recombinant Leishmania P2 acidic ribosomal proteins.

M Soto 1, J M Requena 1, L Quijada 1, C Alonso 1
PMCID: PMC170355  PMID: 8807201

Abstract

The Leishmania P2 proteins have been analyzed as potential tools for the immunodiagnosis of human mucocutaneous and visceral leishmaniasis. Two recombinant Leishmania infantum proteins, rLIP2a and rLip2b, were used. The analysis indicated that the rLiP2a and rLiP2b proteins are recognized by 76% (16 of 21) and 42% (9 of 21), respectively, of sera from patients with mucocutaneous leishmaniasis and by 50% (5 of 10) and 40% (4 of 10), respectively, of sera from patients with visceral leishmaniasis. The Leishmania P2 proteins were engineered to have deletions of particular amino acids from the carboxyl-terminal region in order to avoid cross-reactivity with sera from patients with systemic lupus erythematosus and Chagas' disease, since it is known that this region is the main target of the autoantibodies present in sera from these patients. The results show that while the modified recombinant proteins rLiP2a-Q and rLiP2b-Q, in which the five carboxyl-terminal amino acids had been deleted, maintain the leishmaniasis-specific epitopes, they do not react with sera from patients with autoimmune disease and Chagas' disease. For this reason, and also because the sera from patients with tuberculosis and leprosy, diseases that have to be considered in a differential clinical diagnosis of infectious diseases, do not react with the rLiP2a-Q or rLiP2b-Q protein, we think that the engineered proteins may be considered specific tools for the immunodiagnosis of mucocutaneous and visceral leishmaniasis.

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Selected References

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  1. Ashford R. W., Desjeux P., Deraadt P. Estimation of population at risk of infection and number of cases of Leishmaniasis. Parasitol Today. 1992 Mar;8(3):104–105. doi: 10.1016/0169-4758(92)90249-2. [DOI] [PubMed] [Google Scholar]
  2. Bray R. S. Leishmania. Annu Rev Microbiol. 1974;28(0):189–217. doi: 10.1146/annurev.mi.28.100174.001201. [DOI] [PubMed] [Google Scholar]
  3. Burns J. M., Jr, Shreffler W. G., Benson D. R., Ghalib H. W., Badaro R., Reed S. G. Molecular characterization of a kinesin-related antigen of Leishmania chagasi that detects specific antibody in African and American visceral leishmaniasis. Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):775–779. doi: 10.1073/pnas.90.2.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Elkon K., Bonfa E., Llovet R., Danho W., Weissbach H., Brot N. Properties of the ribosomal P2 protein autoantigen are similar to those of foreign protein antigens. Proc Natl Acad Sci U S A. 1988 Jul;85(14):5186–5189. doi: 10.1073/pnas.85.14.5186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Elkon K., Skelly S., Parnassa A., Moller W., Danho W., Weissbach H., Brot N. Identification and chemical synthesis of a ribosomal protein antigenic determinant in systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7419–7423. doi: 10.1073/pnas.83.19.7419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gicheru M. M., Olobo J. O. Evaluation of recombinant gp63, the major Leishmania surface glycoprotein, as a diagnostic molecule for leishmaniasis in vervet monkeys. Acta Trop. 1994 Dec;58(3-4):345–348. doi: 10.1016/0001-706x(94)90028-0. [DOI] [PubMed] [Google Scholar]
  7. Jaffe C. L., Zalis M. Use of purified parasite proteins from Leishmania donovani for the rapid serodiagnosis of visceral leishmaniasis. J Infect Dis. 1988 Jun;157(6):1212–1220. doi: 10.1093/infdis/157.6.1212. [DOI] [PubMed] [Google Scholar]
  8. Kar K. Serodiagnosis of leishmaniasis. Crit Rev Microbiol. 1995;21(2):123–152. doi: 10.3109/10408419509113537. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Levin M. J., Vazquez M., Kaplan D., Schijman A. G. The Trypanosoma cruzi ribosomal P protein family: classification and antigenicity. Parasitol Today. 1993 Oct;9(10):381–384. doi: 10.1016/0169-4758(93)90088-w. [DOI] [PubMed] [Google Scholar]
  11. Mesri E. A., Levitus G., Hontebeyrie-Joskowicz M., Dighiero G., Van Regenmortel M. H., Levin M. J. Major Trypanosoma cruzi antigenic determinant in Chagas' heart disease shares homology with the systemic lupus erythematosus ribosomal P protein epitope. J Clin Microbiol. 1990 Jun;28(6):1219–1224. doi: 10.1128/jcm.28.6.1219-1224.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Qu J. Q., Zhong L., Masoom-Yasinzai M., Abdur-Rab M., Aksu H. S., Reed S. G., Chang K. P., Gilman-Sachs A. Serodiagnosis of Asian leishmaniasis with a recombinant antigen from the repetitive domain of a Leishmania kinesin. Trans R Soc Trop Med Hyg. 1994 Sep-Oct;88(5):543–545. doi: 10.1016/0035-9203(94)90154-6. [DOI] [PubMed] [Google Scholar]
  13. Quijada L., Requena J. M., Soto M., Alonso C. During canine viscero-cutaneous leishmaniasis the anti-Hsp70 antibodies are specifically elicited by the parasite protein. Parasitology. 1996 Mar;112(Pt 3):277–284. doi: 10.1017/s0031182000065793. [DOI] [PubMed] [Google Scholar]
  14. Reed S. G., Shreffler W. G., Burns J. M., Jr, Scott J. M., Orge M. da G., Ghalib H. W., Siddig M., Badaro R. An improved serodiagnostic procedure for visceral leishmaniasis. Am J Trop Med Hyg. 1990 Dec;43(6):632–639. doi: 10.4269/ajtmh.1990.43.632. [DOI] [PubMed] [Google Scholar]
  15. Shreffler W. G., Burns J. M., Jr, Badaró R., Ghalib H. W., Button L. L., McMaster W. R., Reed S. G. Antibody responses of visceral leishmaniasis patients to gp63, a major surface glycoprotein of Leishmania species. J Infect Dis. 1993 Feb;167(2):426–430. doi: 10.1093/infdis/167.2.426. [DOI] [PubMed] [Google Scholar]
  16. Skeiky Y. A., Benson D. R., Guderian J. A., Sleath P. R., Parsons M., Reed S. G. Trypanosoma cruzi acidic ribosomal P protein gene family. Novel P proteins encoding unusual cross-reactive epitopes. J Immunol. 1993 Nov 15;151(10):5504–5515. [PubMed] [Google Scholar]
  17. Soto M., Requena J. M., Garcia M., Gómez L. C., Navarrete I., Alonso C. Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania. J Biol Chem. 1993 Oct 15;268(29):21835–21843. [PubMed] [Google Scholar]
  18. Soto M., Requena J. M., Quijada L., Angel S. O., Gomez L. C., Guzman F., Patarroyo M. E., Alonso C. During active viscerocutaneous leishmaniasis the anti-P2 humoral response is specifically triggered by the parasite P proteins. Clin Exp Immunol. 1995 May;100(2):246–252. doi: 10.1111/j.1365-2249.1995.tb03661.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Soto M., Requena J. M., Quijada L., García M., Guzman F., Patarroyo M. E., Alonso C. Mapping of the linear antigenic determinants from the Leishmania infantum histone H2A recognized by sera from dogs with leishmaniasis. Immunol Lett. 1995 Dec;48(3):209–214. doi: 10.1016/0165-2478(95)02473-5. [DOI] [PubMed] [Google Scholar]
  20. Soto M., Requena J. M., Quijada L., Guzman F., Patarroyo M. E., Alonso C. Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis. Immunol Lett. 1995 Nov;48(1):23–28. doi: 10.1016/0165-2478(95)02436-0. [DOI] [PubMed] [Google Scholar]
  21. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  22. Wallace G. R., Ball A. E., MacFarlane J., el Safi S. H., Miles M. A., Kelly J. M. Mapping of a visceral leishmaniasis-specific immunodominant B-cell epitope of Leishmania donovani Hsp70. Infect Immun. 1992 Jul;60(7):2688–2693. doi: 10.1128/iai.60.7.2688-2693.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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