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. 1969 Jul;36(3):437–447. doi: 10.1111/j.1476-5381.1969.tb08000.x

The influence of salicylate on platelets and whole blood adenine nucleotides

D T P Davies, A Hughes, R S Tonks
PMCID: PMC1703607  PMID: 5789804

Abstract

1. The addition of sodium salicylate to freshly withdrawn human blood or native platelet-rich plasma significantly delays platelet aggregation in vitro.

2. The administration of acetylsalicylic acid to human subjects also significantly delays platelet aggregation in their whole blood and there is a short delay in the formation of fibrin but this is not statistically significant.

3. Salicylate, whether added to human blood in vitro as sodium salicylate or given by mouth as acetylsalicylic acid, significantly reduces the platelet clumping activity of adenosine diphosphate (ADP) added to whole blood in vitro.

4. The administration of aspirin in high doses for several days produces a marked increase in the total adenine nucleotide content of whole blood. The percentage of adenosine triphosphate (ATP) was increased, that of ADP decreased while there is an obvious increase in the ATP: ADP ratio.

5. There is little correlation between the plasma salicylate level and the delay aspirin produces in platelet aggregation in vitro or the changes that occur in the levels of ADP or ATP in whole blood during administration of aspirin.

6. Significant correlations do occur, however, between the delay in platelet aggregation in vitro and (i) the percentage increase in the ATP concentration, (ii) the percentage decrease in ADP concentration, (iii) the percentage change in the ATP: ADP ratio observed during aspirin administration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BORN G. V. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. 1962 Jun 9;194:927–929. doi: 10.1038/194927b0. [DOI] [PubMed] [Google Scholar]
  2. BRYANT C., SMITH M. J., HINES W. J. Effects of salicylate and gamma-resorcylate on the metabolism of radioactive succinate and fumarate by rat-liver mitochondria and on dehydrogenase enzymes. Biochem J. 1963 Mar;86:391–396. doi: 10.1042/bj0860391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Caspary E. A. Effect of red blood-cells and adenosine on platelet adhesiveness. Lancet. 1965 Dec 18;2(7425):1273–1273. doi: 10.1016/s0140-6736(65)92285-3. [DOI] [PubMed] [Google Scholar]
  4. Davies D. T., Hughes A., Tonks R. S. Salicylates, platelets and adenine nucleotides. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1968;259(2):163–164. doi: 10.1007/BF00537757. [DOI] [PubMed] [Google Scholar]
  5. Dawkins P. D., Gould B. J., Sturman J. A., Smith M. J. The mechanism of the inhibition of dehydrogenases by salicylate. J Pharm Pharmacol. 1967 Jun;19(6):355–366. doi: 10.1111/j.2042-7158.1967.tb09561.x. [DOI] [PubMed] [Google Scholar]
  6. GAARDER A., JONSEN J., LALAND S., HELLEM A., OWREN P. A. Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets. Nature. 1961 Nov 11;192:531–532. doi: 10.1038/192531a0. [DOI] [PubMed] [Google Scholar]
  7. GOULD B. J., HUGGINS A. K., SMITH M. J. EFFECTS OF SALICYLATE ON GLUTAMATE DEHYDROGENASE AND GLUTAMATE DECARBOXYLASE. Biochem J. 1963 Aug;88:346–349. doi: 10.1042/bj0880346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HELLEM A. J., BORCHGREVINK C. F., AMES S. B. The role of red cells in haemostasis: the relation between haematocrit, bleeding time and platelet adhesiveness. Br J Haematol. 1961 Jan;7:42–50. doi: 10.1111/j.1365-2141.1961.tb00318.x. [DOI] [PubMed] [Google Scholar]
  9. HINES W. J., SMITH M. J. INHIBITION OF DEHYDROGENASES BY SALICYLATE. Nature. 1964 Jan 11;201:192–192. doi: 10.1038/201192a0. [DOI] [PubMed] [Google Scholar]
  10. HUGHES A., TONKS R. S. PLATELETS, MAGNESIUM, AND MYOCARDIAL INFARCTION. Lancet. 1965 May 15;1(7394):1044–1046. doi: 10.1016/s0140-6736(65)91317-6. [DOI] [PubMed] [Google Scholar]
  11. Harrison M. J., Mitchell J. R. The influence of red blood-cells on platelet adhesiveness. Lancet. 1966 Nov 26;2(7474):1163–1164. doi: 10.1016/s0140-6736(66)90478-8. [DOI] [PubMed] [Google Scholar]
  12. Hughes A., Tonks R. S. Lung and heart lesions from intravascular platelet clumping and its sequelae. J Pathol Bacteriol. 1968 Apr;95(2):523–526. doi: 10.1002/path.1700950225. [DOI] [PubMed] [Google Scholar]
  13. Hughes A., Tonks R. S. Magnesium, adenosine diphosphate and blood platelets. Nature. 1966 Apr 2;210(5031):106–107. doi: 10.1038/210106a0. [DOI] [PubMed] [Google Scholar]
  14. MacMillan D. C. Effect of salicylates on human platelets. Lancet. 1968 May 25;1(7552):1151–1151. doi: 10.1016/s0140-6736(68)90211-0. [DOI] [PubMed] [Google Scholar]
  15. O'Brien J. R. Effects of salicylates on human platelets. Lancet. 1968 Apr 13;1(7546):779–783. doi: 10.1016/s0140-6736(68)92228-9. [DOI] [PubMed] [Google Scholar]
  16. Rorvik T. O., Holmsen I., Stormorken H. The release of ADP from red blood cells. Thromb Diath Haemorrh. 1968 Mar 31;19(1):77–83. [PubMed] [Google Scholar]
  17. SMITH M. J., BRYANT C., HINES W. J. REVERSAL BY NICOTINAMIDE ADENINE DINUCLEOTIDE OF THE INHIBITORY ACTION OF SALICYLATE ON MITOCHONDRIAL MALATE DEHYDROGENASE. Nature. 1964 Apr 4;202:96–97. doi: 10.1038/202096a0. [DOI] [PubMed] [Google Scholar]
  18. TRINDER P. Rapid determination of salicylate in biological fluids. Biochem J. 1954 Jun;57(2):301–303. doi: 10.1042/bj0570301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weiss H. J., Aledort L. M. Impaired platelet-connective-tissue reaction in man after aspirin ingestion. Lancet. 1967 Sep 2;2(7514):495–497. doi: 10.1016/s0140-6736(67)91658-3. [DOI] [PubMed] [Google Scholar]
  20. Zucker M. B., Peterson J. Inhibition of adenosine diphosphate-induced secondary aggregation and other platelet functions by acetylsalicylic acid ingestion. Proc Soc Exp Biol Med. 1968 Feb;127(2):547–551. doi: 10.3181/00379727-127-32737. [DOI] [PubMed] [Google Scholar]

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