Skip to main content
PMC home page
British Journal of Pharmacology and Chemotherapy logoLink to British Journal of Pharmacology and Chemotherapy
. 1963 Apr;20(2):278–284. doi: 10.1111/j.1476-5381.1963.tb01467.x

Dopamine-β-oxidase activity in man, using hydroxyamphetamine as substrate

A Sjoerdsma, W von Studnitz
PMCID: PMC1703637  PMID: 13977820

Abstract

Hydroxyamphetamine was administered orally to five human subjects in daily doses of 26 to 118 mg. Approximately half the dosage administered was recoverable in the urine as unchanged drug (free plus conjugated) and 3.7 to 9.1% was excreted as the β-hydroxylated metabolite, hydroxynorephedrine (free plus conjugated). Since conversion of hydroxyamphetamine to hydroxynorephedrine occurs in vitro by the action of dopamine-β-oxidase, a simple method is suggested for measuring the activity of this enzyme and the effect of its inhibitors in man. No impairment of β-hydroxylation was noted in an adrenalectomized subject. The β-hydroxylation of hydroxyamphetamine in vivo has not been described previously.

Full text

PDF
278

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AXELROD J. Studies on sympathomimetic amines. II. The biotransformation and physiological disposition of d-amphetamine, d-p-hydroxyamphetamine and d-methamphetamine. J Pharmacol Exp Ther. 1954 Mar;110(3):315–326. [PubMed] [Google Scholar]
  2. BRIDGERS W. F., KAUFMAN S. The enzymatic conversion of epinine to epinephrine. J Biol Chem. 1962 Feb;237:526–528. [PubMed] [Google Scholar]
  3. CREVELING C. R., DALY J. W., WITKOP B., UNDENFRIEND S. Substrates and inhibitors of dopamine-beta-oxidase. Biochim Biophys Acta. 1962 Oct 8;64:125–134. doi: 10.1016/0006-3002(62)90765-5. [DOI] [PubMed] [Google Scholar]
  4. CREVELING C. R., VAN DER SCHOOT J. B., UDENFRIEND S. Phenethylamine isosteres as inhibitors of dopamine beta-oxidase. Biochem Biophys Res Commun. 1962 Jul 3;8:215–219. doi: 10.1016/0006-291x(62)90266-8. [DOI] [PubMed] [Google Scholar]
  5. FALES H. M., PISANO J. J. Gas chromatography of biologically important amines. Anal Biochem. 1962 Apr;3:337–342. doi: 10.1016/0003-2697(62)90118-5. [DOI] [PubMed] [Google Scholar]
  6. GOLDSTEIN M., CONTRERA J. F. The substrate specificity of phenylamine-beta-hydroxylase. J Biol Chem. 1962 Jun;237:1898–1902. [PubMed] [Google Scholar]
  7. HESS S. M., CONNAMACHER R. H., OZAKI M., UDENFRIEND S. The effects of alpha-methyl-DOPA and alpha-methyl-metatyrosine on the metabolism of norepinephrine and serotonin in vivo. J Pharmacol Exp Ther. 1961 Nov;134:129–138. [PubMed] [Google Scholar]
  8. KUNTZMAN R., COSTA E., CREVELING C., HIRSCH C. W., BRODIE B. B. Inhibition of norepinephrine synthesis in mouse brain by blockade of dopaminebeta-oxidase. Life Sci. 1962 Mar;1:85–92. doi: 10.1016/0024-3205(62)90034-6. [DOI] [PubMed] [Google Scholar]
  9. LEVIN E. Y., LEVENBERG B., KAUFMAN S. The enzymatic conversion of 3,4-dihydroxyphenylethylamine to norepinephrine. J Biol Chem. 1960 Jul;235:2080–2086. [PubMed] [Google Scholar]
  10. PISANO J. J. A simple analysis for normetanephrine and metanephrine in urine. Clin Chim Acta. 1960 May;5:406–414. doi: 10.1016/0009-8981(60)90146-7. [DOI] [PubMed] [Google Scholar]
  11. PISANO J. J., OATES J. A., Jr, KARMEN A., SJOERDSMA A., UDENFRIEND S. Identification of p-hydroxy-alpha-(methylaminomethyl) benzyl alcohol (synephrine) in human urine. J Biol Chem. 1961 Mar;236:898–901. [PubMed] [Google Scholar]
  12. UDENFRIEND S., CREVELING C. R. Localization of dopamine-beta-oxidase in brain. J Neurochem. 1959 Oct;4:350–352. doi: 10.1111/j.1471-4159.1959.tb13216.x. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology and Chemotherapy are provided here courtesy of The British Pharmacological Society

RESOURCES