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. 1996 Jul;3(4):464–469. doi: 10.1128/cdli.3.4.464-469.1996

Effect of gamma interferon on expression of Fc gamma receptors in monocytes of newborn infants and adults.

K D Fairchild 1, R G Hudson 1, S D Douglas 1, S E McKenzie 1, R A Polin 1
PMCID: PMC170368  PMID: 8807214

Abstract

Fc gamma receptors provide an essential link between cellular and humoral immunity, and little is known about their expression in monocytes of newborn infants. We compared baseline and gamma interferon (IFN-gamma)-induced expression of Fc gamma RI and Fc gamma RII protein and Fc gamma RI mRNA in monocytes from healthy, term infants and adults. Fluorescence-activated cell sorter analysis demonstrated that baseline expression of monocyte Fc gamma RI in newborn infants was not significantly different from that in adults, while Fc gamma RII protein expression in monocytes derived from newborns was significantly higher than that for adults (mean channel fluorescence [MCF] for newborns and adults, 5.53 and 4.50, respectively [P = 0.039]). In vitro treatment with recombinant IFN-gamma increased the expression of Fc gamma RI in monocytes of newborns and adults to the same extent (2.4- and 2.2-fold increase in MCF in newborns and adults, respectively, at 42 h). We developed a semiquantitative fluorescence reverse transcriptase PCR which demonstrated a significant increase in mRNA for Fc gamma RI in monocytes of newborns and adults with in vitro IFN-gamma exposure, indicating that IFN-gamma acts by increasing the transcription or transcript stability of Fc gamma RI mRNA. While there was no significant effect of IFN-gamma treatment on Fc gamma RII expression in monocytes from adults, there was a 20% increase in Fc gamma RII in monocytes from newborns (P = 0.009). Monocytes from healthy, term newborns and adults exhibit comparable baseline and IFN-gamma-induced levels of expression of Fc gamma RI and higher baseline and IFN-gamma-induced levels of expression of Fc gamma RII.

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Selected References

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  1. Adams D. O., Hamilton T. A. Molecular transductional mechanisms by which IFN gamma and other signals regulate macrophage development. Immunol Rev. 1987 Jun;97:5–27. doi: 10.1111/j.1600-065x.1987.tb00514.x. [DOI] [PubMed] [Google Scholar]
  2. Bryson Y. J., Winter H. S., Gard S. E., Fischer T. J., Stiehm E. R. Deficiency of immune interferon production by leukocytes of normal newborns. Cell Immunol. 1980 Sep 15;55(1):191–200. doi: 10.1016/0008-8749(80)90150-1. [DOI] [PubMed] [Google Scholar]
  3. Carr R., Davies J. M. Abnormal FcRIII expression by neutrophils from very preterm neonates. Blood. 1990 Aug 1;76(3):607–611. [PubMed] [Google Scholar]
  4. Carr R., Huizinga T. W., Kleijer M., Davies J. M. Changes in plasma FcRIII demonstrate increasing receptor production during late pregnancy and after preterm birth. Pediatr Res. 1992 Nov;32(5):505–508. doi: 10.1203/00006450-199211000-00001. [DOI] [PubMed] [Google Scholar]
  5. Comber P. G., Lentz V., Schreiber A. D. Modulation of the transcriptional rate of Fc gamma receptor mRNA in human mononuclear phagocytes. Cell Immunol. 1992 Dec;145(2):324–338. doi: 10.1016/0008-8749(92)90335-m. [DOI] [PubMed] [Google Scholar]
  6. Comber P. G., Rossman M. D., Rappaport E. F., Chien P., Hogarth P. M., Schreiber A. D. Modulation of human mononuclear phagocyte Fc gamma RII mRNA and protein. Cell Immunol. 1989 Dec;124(2):292–307. doi: 10.1016/0008-8749(89)90132-9. [DOI] [PubMed] [Google Scholar]
  7. English B. K., Burchett S. K., English J. D., Ammann A. J., Wara D. W., Wilson C. B. Production of lymphotoxin and tumor necrosis factor by human neonatal mononuclear cells. Pediatr Res. 1988 Dec;24(6):717–722. doi: 10.1203/00006450-198812000-00014. [DOI] [PubMed] [Google Scholar]
  8. Erbe D. V., Collins J. E., Shen L., Graziano R. F., Fanger M. W. The effect of cytokines on the expression and function of Fc receptors for IgG on human myeloid cells. Mol Immunol. 1990 Jan;27(1):57–67. doi: 10.1016/0161-5890(90)90060-d. [DOI] [PubMed] [Google Scholar]
  9. Ernst L. K., Duchemin A. M., Anderson C. L. Association of the high-affinity receptor for IgG (Fc gamma RI) with the gamma subunit of the IgE receptor. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6023–6027. doi: 10.1073/pnas.90.13.6023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Guyre P. M., Morganelli P. M., Miller R. Recombinant immune interferon increases immunoglobulin G Fc receptors on cultured human mononuclear phagocytes. J Clin Invest. 1983 Jul;72(1):393–397. doi: 10.1172/JCI110980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ho W. Z., Lioy J., Song L., Cutilli J. R., Polin R. A., Douglas S. D. Infection of cord blood monocyte-derived macrophages with human immunodeficiency virus type 1. J Virol. 1992 Jan;66(1):573–579. doi: 10.1128/jvi.66.1.573-579.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Indik Z., Kelly C., Chien P., Levinson A. I., Schreiber A. D. Human Fc gamma RII, in the absence of other Fc gamma receptors, mediates a phagocytic signal. J Clin Invest. 1991 Nov;88(5):1766–1771. doi: 10.1172/JCI115496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jungi T. W., Lerch P. G., Brcic M. The effect of recombinant interferon-gamma on human monocyte-derived macrophages. Eur J Immunol. 1987 May;17(5):735–738. doi: 10.1002/eji.1830170526. [DOI] [PubMed] [Google Scholar]
  14. Keller M. A., Cassel D. L., Rappaport E. F., McKenzie S. E., Schwartz E., Surrey S. Fluorescence-based RT PCR analysis: determination of the ratio of soluble to membrane-bound forms of Fc gamma RIIA transcripts in hematopoietic cell lines. PCR Methods Appl. 1993 Aug;3(1):32–38. doi: 10.1101/gr.3.1.32. [DOI] [PubMed] [Google Scholar]
  15. Liesveld J. L., Abboud C. N., Looney R. J., Ryan D. H., Brennan J. K. Expression of IgG Fc receptors in myeloid leukemic cell lines. Effect of colony-stimulating factors and cytokines. J Immunol. 1988 Mar 1;140(5):1527–1533. [PubMed] [Google Scholar]
  16. Maródi L., Káposzta R., Campbell D. E., Polin R. A., Csongor J., Johnston R. B., Jr Candidacidal mechanisms in the human neonate. Impaired IFN-gamma activation of macrophages in newborn infants. J Immunol. 1994 Dec 15;153(12):5643–5649. [PubMed] [Google Scholar]
  17. Maródi L., Leijh P. C., van Furth R. Characteristics and functional capacities of human cord blood granulocytes and monocytes. Pediatr Res. 1984 Nov;18(11):1127–1131. doi: 10.1203/00006450-198411000-00014. [DOI] [PubMed] [Google Scholar]
  18. McKenzie S. E., Kline J., Douglas S. D., Polin R. A. Enhancement in vitro of the low interferon-gamma production of leukocytes from human newborn infants. J Leukoc Biol. 1993 Jun;53(6):691–696. doi: 10.1002/jlb.53.6.691. [DOI] [PubMed] [Google Scholar]
  19. McKenzie S. E., Schreiber A. D. Biological advances and clinical applications of Fc receptors for IgG. Curr Opin Hematol. 1994 Jan;1(1):45–52. [PubMed] [Google Scholar]
  20. Pan L. Y., Mendel D. B., Zurlo J., Guyre P. M. Regulation of the steady state level of Fc gamma RI mRNA by IFN-gamma and dexamethasone in human monocytes, neutrophils, and U-937 cells. J Immunol. 1990 Jul 1;145(1):267–275. [PubMed] [Google Scholar]
  21. Payne N. R., Frestedt J., Hunkeler N., Gehrz R. Cell-surface expression of immunoglobulin G receptors on the polymorphonuclear leukocytes and monocytes of extremely premature infants. Pediatr Res. 1993 May;33(5):452–457. doi: 10.1203/00006450-199305000-00007. [DOI] [PubMed] [Google Scholar]
  22. Pearse R. N., Feinman R., Ravetch J. V. Characterization of the promoter of the human gene encoding the high-affinity IgG receptor: transcriptional induction by gamma-interferon is mediated through common DNA response elements. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11305–11309. doi: 10.1073/pnas.88.24.11305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Perussia B., Dayton E. T., Lazarus R., Fanning V., Trinchieri G. Immune interferon induces the receptor for monomeric IgG1 on human monocytic and myeloid cells. J Exp Med. 1983 Oct 1;158(4):1092–1113. doi: 10.1084/jem.158.4.1092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Petroni K. C., Shen L., Guyre P. M. Modulation of human polymorphonuclear leukocyte IgG Fc receptors and Fc receptor-mediated functions by IFN-gamma and glucocorticoids. J Immunol. 1988 May 15;140(10):3467–3472. [PubMed] [Google Scholar]
  25. Rappaport E. F., Cassel D. L., Walterhouse D. O., McKenzie S. E., Surrey S., Keller M. A., Schreiber A. D., Schwartz E. A soluble form of the human Fc receptor Fc gamma RIIA: cloning, transcript analysis and detection. Exp Hematol. 1993 May;21(5):689–696. [PubMed] [Google Scholar]
  26. Ravetch J. V. Fc receptors: rubor redux. Cell. 1994 Aug 26;78(4):553–560. doi: 10.1016/0092-8674(94)90521-5. [DOI] [PubMed] [Google Scholar]
  27. Smith J. B., Campbell D. E., Ludomirsky A., Polin R. A., Douglas S. D., Garty B. Z., Harris M. C. Expression of the complement receptors CR1 and CR3 and the type III Fc gamma receptor on neutrophils from newborn infants and from fetuses with Rh disease. Pediatr Res. 1990 Aug;28(2):120–126. doi: 10.1203/00006450-199008000-00009. [DOI] [PubMed] [Google Scholar]
  28. Takahashi N., Nishida H., Kuratsuji T. Fc gamma RI and Fc gamma RIII on polymorphonuclear leucocytes in cord blood. Arch Dis Child Fetal Neonatal Ed. 1994 Jan;70(1):F31–F35. doi: 10.1136/fn.70.1.f31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Takai T., Li M., Sylvestre D., Clynes R., Ravetch J. V. FcR gamma chain deletion results in pleiotrophic effector cell defects. Cell. 1994 Feb 11;76(3):519–529. doi: 10.1016/0092-8674(94)90115-5. [DOI] [PubMed] [Google Scholar]
  30. Wakasugi N., Virelizier J. L. Defective IFN-gamma production in the human neonate. I. Dysregulation rather than intrinsic abnormality. J Immunol. 1985 Jan;134(1):167–171. [PubMed] [Google Scholar]
  31. Warren M. K., Vogel S. N. Opposing effects of glucocorticoids on interferon-gamma-induced murine macrophage Fc receptor and Ia antigen expression. J Immunol. 1985 Apr;134(4):2462–2469. [PubMed] [Google Scholar]
  32. Wilson C. B., Westall J. Activation of neonatal and adult human macrophages by alpha, beta, and gamma interferons. Infect Immun. 1985 Aug;49(2):351–356. doi: 10.1128/iai.49.2.351-356.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wilson C. B., Westall J., Johnston L., Lewis D. B., Dower S. K., Alpert A. R. Decreased production of interferon-gamma by human neonatal cells. Intrinsic and regulatory deficiencies. J Clin Invest. 1986 Mar;77(3):860–867. doi: 10.1172/JCI112383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wilson K. C., Finbloom D. S. Interferon gamma rapidly induces in human monocytes a DNA-binding factor that recognizes the gamma response region within the promoter of the gene for the high-affinity Fc gamma receptor. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11964–11968. doi: 10.1073/pnas.89.24.11964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Wirthmueller U., Kurosaki T., Murakami M. S., Ravetch J. V. Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain. J Exp Med. 1992 May 1;175(5):1381–1390. doi: 10.1084/jem.175.5.1381. [DOI] [PMC free article] [PubMed] [Google Scholar]

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