Skip to main content
British Journal of Pharmacology and Chemotherapy logoLink to British Journal of Pharmacology and Chemotherapy
. 1963 Dec;21(3):482–490. doi: 10.1111/j.1476-5381.1963.tb02016.x

The subcellular localization of dopamine and acetylcholine in the dog caudate nucleus

R Laverty, I A Michaelson, D F Sharman, V P Whittaker
PMCID: PMC1703886  PMID: 14110748

Abstract

The distribution of dopamine (3,4-dihydroxyphenylethylamine) between the subcellular fractions from homogenates of dog caudate nucleus was compared with the distributions of acetylcholine, 5-hydroxytryptamine and lactate dehydrogenase activity. The distributions of noradrenaline and 5-hydroxytryptamine between the subcellular fractions from homogenates of dog hypothalamus were also determined. Most of the dopamine, in contrast to acetylcholine, occurred in the soluble supernatant fraction; the remainder was associated with the fractions rich in pinched-off nerve ending particles, but localization in any one fraction was not as sharp as that of acetylcholine. Evidence is presented that suggests that the dopamine occurs in a free or easily released form throughout cell cytoplasm.

Full text

PDF
482

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALDRIDGE W. N., EMERY R. C., STREET B. W. A tissue homogenizer. Biochem J. 1960 Nov;77:326–327. doi: 10.1042/bj0770326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ASHCROFT G. W., SHARMAN D. F. Drug-induced changes in the concentration of 5-OR indolyl compounds in cerebrospinal fluid and caudate nucleus. Br J Pharmacol Chemother. 1962 Aug;19:153–160. doi: 10.1111/j.1476-5381.1962.tb01436.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BERTLER A., CARLSSON A., ROSENGREN E. A method for the fluorimetric determination of adrenaline and noradrenaline in tissues. Acta Physiol Scand. 1958 Dec 15;44(3-4):273–292. doi: 10.1111/j.1748-1716.1958.tb01627.x. [DOI] [PubMed] [Google Scholar]
  4. BERTLER A., ROSENGREN E. Occurrence and distribution of catechol amines in brain. Acta Physiol Scand. 1959 Dec 12;47:350–361. [PubMed] [Google Scholar]
  5. BRODKIN E., ELLIOTT K. A. Binding of acetylcholine. Am J Physiol. 1953 Jun;173(3):437–442. doi: 10.1152/ajplegacy.1953.173.3.437. [DOI] [PubMed] [Google Scholar]
  6. GRAY E. G., WHITTAKER V. P. The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J Anat. 1962 Jan;96:79–88. [PMC free article] [PubMed] [Google Scholar]
  7. HEBB C. O., WHITTAKER V. P. Intracellular distributions of acetylcholine and choline acetylase. J Physiol. 1958 Jun 18;142(1):187–196. doi: 10.1113/jphysiol.1958.sp006008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HORNE R. W., WHITTAKER V. P. The use of the negative staining method for the electron-microscopic study of subcellular particles from animal tissues. Z Zellforsch Mikrosk Anat. 1962;58:1–16. doi: 10.1007/BF00406937. [DOI] [PubMed] [Google Scholar]
  9. JOHNSON M. K. The intracellular distribution of glycolytic and other enzymes in rat-brain homogenates and mitochondrial preparations. Biochem J. 1960 Dec;77:610–618. doi: 10.1042/bj0770610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. JOHNSON M. K., WHITTAKER V. P. LACTATE DEHYDROGENASE AS A CYTOPLASMIC MARKER IN BRAIN. Biochem J. 1963 Sep;88:404–409. doi: 10.1042/bj0880404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MICHAELSON I. A., WHITTAKER V. P. The subcellular localization of 5-hydroxytryptamine in guinea pig brain. Biochem Pharmacol. 1963 Feb;12:203–211. doi: 10.1016/0006-2952(63)90185-0. [DOI] [PubMed] [Google Scholar]
  12. SCHUMANN H. J. Uber die Verteilung von Noradrenalin und Hydroxytyramin in sympathischen Nerven (Milznerven). Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1958;234(1):17–25. [PubMed] [Google Scholar]
  13. SHARMAN D. F. A fluorimetric method for the estimation of 4-hydroxy-3-methoxyphenylacetic acid (homovanillic acid) and its identification in brain tissue. Br J Pharmacol Chemother. 1963 Feb;20:204–213. doi: 10.1111/j.1476-5381.1963.tb01310.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SHARMAN D. F., VANOV S., VOGT M. Noradrenaline content in the heart and spleen of the mouse under normal conditions and after administration of some drugs. Br J Pharmacol Chemother. 1962 Dec;19:527–533. doi: 10.1111/j.1476-5381.1962.tb01458.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. VOGT M. The concentration of sympathin in different parts of the central nervous system under normal conditions and after the administration of drugs. J Physiol. 1954 Mar 29;123(3):451–481. doi: 10.1113/jphysiol.1954.sp005064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WEIL-MALHERBE H., BONE A. D. Intracellular distribution of catecholamines in the brain. Nature. 1957 Nov 16;180(4594):1050–1051. doi: 10.1038/1801050a0. [DOI] [PubMed] [Google Scholar]
  17. WEIL-MALHERBE H., BONE A. D. The chemical estimation of adrenaline-like substances in blood. Biochem J. 1952 Jun;51(3):311–318. doi: 10.1042/bj0510311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WEIL-MALHERBE H., BONE A. D. The effect of reserpine on the intracellular distribution of catecholamines in the brain stem of the rabbit. J Neurochem. 1959 Aug;4:251–263. doi: 10.1111/j.1471-4159.1959.tb13201.x. [DOI] [PubMed] [Google Scholar]
  19. WEIL-MALHERBE H., BONE A. D. The estimation of catecholamines in urine by a chemical method. J Clin Pathol. 1957 May;10(2):138–147. doi: 10.1136/jcp.10.2.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WEIL-MALHERBE H., POSNER H. S., BOWLES G. R. Changes in the concentration and intracellular distribution of brain catecholamines: the effects of reserpine. beta-phenyliso-propylhydrazine, pyrogallol and 3,4-dihydroxyphenyl alanine, alone and in combination. J Pharmacol Exp Ther. 1961 Jun;132:278–286. [PubMed] [Google Scholar]
  21. WHITTAKER V. P., MICHAELSON I. A., KIRKLAND R. J. The separation of synaptic vesicles from disrupted nervending particles. Biochem Pharmacol. 1963 Mar;12:300–302. doi: 10.1016/0006-2952(63)90156-4. [DOI] [PubMed] [Google Scholar]
  22. WHITTAKER V. P. The isolation and characterization of acetylcholine-containing particles from brain. Biochem J. 1959 Aug;72:694–706. doi: 10.1042/bj0720694. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology and Chemotherapy are provided here courtesy of The British Pharmacological Society

RESOURCES