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British Journal of Pharmacology and Chemotherapy logoLink to British Journal of Pharmacology and Chemotherapy
. 1964 Feb;22(1):183–188. doi: 10.1111/j.1476-5381.1964.tb01557.x

Thin-layer chromatography and bioassay of prostaglandins in extracts of semen and tissues of the male reproductive tract

E W Horton, C J Thompson
PMCID: PMC1703900  PMID: 14126049

Abstract

By acid ether extraction and thin-layer chromatography, prostaglandins have been separated from biologically active compounds of other chemical groups. The technique does not, however, separate different prostaglandins from each other. The biological activity of the eluates was estimated on the rabbit isolated jejunum and the hamster isolated colon preparations in terms of prostaglandin E1; the concentrations (thus expressed) of prostaglandin in human semen ranged from 24 to 783 μg/ml. with a mean of 226 μg/ml. No prostaglandins (minimal detectable concentration, 0.5 μg/g) could be detected in the male reproductive organs of several species of laboratory animal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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