Skip to main content
NCBI home page
Clinical and Diagnostic Laboratory Immunology logoLink to Clinical and Diagnostic Laboratory Immunology
. 1996 Sep;3(5):500–506. doi: 10.1128/cdli.3.5.500-506.1996

Antibody responses against the G and F proteins of bovine respiratory syncytial virus after experimental and natural infections.

R S Schrijver 1, J P Langedijk 1, W H van der Poel 1, W G Middel 1, J A Kramps 1, J T van Oirschot 1
PMCID: PMC170396  PMID: 8877125

Abstract

Antibodies against the two major surface glycoproteins of bovine respiratory syncytial virus (BRSV), G and F, play a role in protection against BRSV-associated disease, but only the antibody response against the F protein has been well described. Therefore, we used a novel peptide-based enzyme-linked immunosorbent assay (G peptide-ELISA) to compare immunoglobulin G (IgG) and IgG subclass antibody responses against the G protein with the antibody response against the F protein, as measured by a conventional BRSV ELISA (F-ELISA). Experimental infection of cattle induced significantly lower antibody titers than did natural infection. After natural primary infection, G peptide-specific antibodies declined more rapidly and to lower levels than the F protein-specific antibodies. As a consequence, the G peptide-ELISA detected more reinfections than did the F-ELISA. Ratios of G- and F-specific IgG1/IgG2 antibody titers did not differ markedly after infection or vaccination. Interestingly, after natural infection calves did not develop an IgG2 response to the complete G protein. In contrast, adult cattle had high IgG2 titers against this protein. Vaccination with a live vaccine induced low antibody titers, similar to the titers after experimental infection, whereas vaccination with an inactivated vaccine induced high titers. The results indicate that the kinetics of the G- and F-specific antibody responses differ. Furthermore, the IgG subclass response against the unglycosylated central region of the G protein is similar to the IgG subclass response to the F protein, but the IgG subclass response differs from the response to the complete G protein.

Full Text

The Full Text of this article is available as a PDF (215.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson L. J., Heilman C. A. Protective and disease-enhancing immune responses to respiratory syncytial virus. J Infect Dis. 1995 Jan;171(1):1–7. doi: 10.1093/infdis/171.1.1. [DOI] [PubMed] [Google Scholar]
  2. Belknap E. B., Ciszewski D. K., Baker J. C. Experimental respiratory syncytial virus infection in calves and lambs. J Vet Diagn Invest. 1995 Apr;7(2):285–298. doi: 10.1177/104063879500700226. [DOI] [PubMed] [Google Scholar]
  3. Brüggemann M., Free J., Diamond A., Howard J., Cobbold S., Waldmann H. Immunoglobulin heavy chain locus of the rat: striking homology to mouse antibody genes. Proc Natl Acad Sci U S A. 1986 Aug;83(16):6075–6079. doi: 10.1073/pnas.83.16.6075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Butler J. E. Bovine immunoglobulins: an augmented review. Vet Immunol Immunopathol. 1983 Mar;4(1-2):43–152. doi: 10.1016/0165-2427(83)90056-9. [DOI] [PubMed] [Google Scholar]
  5. Butler J. E., Navarro P., Heyermann H. Heterogeneity of bovine IgG2. VI. Comparative specificity of monoclonal and polyclonal capture antibodies for IgG2a (A1) and IgG2a (A2). Vet Immunol Immunopathol. 1994 Feb;40(2):119–133. doi: 10.1016/0165-2427(94)90028-0. [DOI] [PubMed] [Google Scholar]
  6. Estes D. M., Hirano A., Heussler V. T., Dobbelaere D. A., Brown W. C. Expression and biological activities of bovine interleukin 4: effects of recombinant bovine interleukin 4 on T cell proliferation and B cell differentiation and proliferation in vitro. Cell Immunol. 1995 Jul;163(2):268–279. doi: 10.1006/cimm.1995.1126. [DOI] [PubMed] [Google Scholar]
  7. Furze J., Wertz G., Lerch R., Taylor G. Antigenic heterogeneity of the attachment protein of bovine respiratory syncytial virus. J Gen Virol. 1994 Feb;75(Pt 2):363–370. doi: 10.1099/0022-1317-75-2-363. [DOI] [PubMed] [Google Scholar]
  8. Hammarström L., Smith C. I. IgG subclasses in bacterial infections. Monogr Allergy. 1986;19:122–133. [PubMed] [Google Scholar]
  9. Heckert R. A., Saif L. J., Mengel J. P., Myers G. W. Isotype-specific antibody responses to bovine coronavirus structural proteins in serum, feces, and mucosal secretions from experimentally challenge-exposed colostrum-deprived calves. Am J Vet Res. 1991 May;52(5):692–699. [PubMed] [Google Scholar]
  10. Kimman T. G., Daha M. R., Brinkhof J. M., Westenbrink F. Activation of complement by bovine respiratory syncytial virus-infected cells. Vet Immunol Immunopathol. 1989 Jul;21(3-4):311–325. doi: 10.1016/0165-2427(89)90039-1. [DOI] [PubMed] [Google Scholar]
  11. Kimman T. G., Westenbrink F. Immunity to human and bovine respiratory syncytial virus. Arch Virol. 1990;112(1-2):1–25. doi: 10.1007/BF01348982. [DOI] [PubMed] [Google Scholar]
  12. Kimman T. G., Westenbrink F., Schreuder B. E., Straver P. J. Local and systemic antibody response to bovine respiratory syncytial virus infection and reinfection in calves with and without maternal antibodies. J Clin Microbiol. 1987 Jun;25(6):1097–1106. doi: 10.1128/jcm.25.6.1097-1106.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kimman T. G., Westenbrink F., Straver P. J. Priming for local and systemic antibody memory responses to bovine respiratory syncytial virus: effect of amount of virus, virus replication, route of administration and maternal antibodies. Vet Immunol Immunopathol. 1989 Sep;22(2):145–160. doi: 10.1016/0165-2427(89)90057-3. [DOI] [PubMed] [Google Scholar]
  14. Kimman T. G., Westenbrink F., Straver P. J., Van Zaane D., Schreuder B. E. Isotype-specific ELISAs for the detection of antibodies to bovine respiratory syncytial virus. Res Vet Sci. 1987 Sep;43(2):180–187. [PubMed] [Google Scholar]
  15. Kramps J. A., Magdalena J., Quak J., Weerdmeester K., Kaashoek M. J., Maris-Veldhuis M. A., Rijsewijk F. A., Keil G., van Oirschot J. T. A simple, specific, and highly sensitive blocking enzyme-linked immunosorbent assay for detection of antibodies to bovine herpesvirus 1. J Clin Microbiol. 1994 Sep;32(9):2175–2181. doi: 10.1128/jcm.32.9.2175-2181.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Madic J., Magdalena J., Quak J., van Oirschot J. T. Isotype-specific antibody responses in sera and mucosal secretions of calves experimentally infected with bovine herpesvirus 1. Vet Immunol Immunopathol. 1995 Jun;46(3-4):267–283. doi: 10.1016/0165-2427(94)05363-w. [DOI] [PubMed] [Google Scholar]
  17. Mulcahy G., Gale C., Robertson P., Iyisan S., DiMarchi R. D., Doel T. R. Isotype responses of infected, virus-vaccinated and peptide-vaccinated cattle to foot-and-mouth disease virus. Vaccine. 1990 Jun;8(3):249–256. doi: 10.1016/0264-410x(90)90054-p. [DOI] [PubMed] [Google Scholar]
  18. Skvaril F. IgG subclasses in viral infections. Monogr Allergy. 1986;19:134–143. [PubMed] [Google Scholar]
  19. Stott E. J., Taylor G. Respiratory syncytial virus. Brief review. Arch Virol. 1985;84(1-2):1–52. doi: 10.1007/BF01310552. [DOI] [PubMed] [Google Scholar]
  20. Sullender W. M., Mufson M. A., Anderson L. J., Wertz G. W. Genetic diversity of the attachment protein of subgroup B respiratory syncytial viruses. J Virol. 1991 Oct;65(10):5425–5434. doi: 10.1128/jvi.65.10.5425-5434.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Van der Poel W. H., Kramps J. A., Middel W. G., Van Oirschot J. T., Brand A. Dynamics of bovine respiratory syncytial virus infections: a longitudinal epidemiological study in dairy herds. Arch Virol. 1993;133(3-4):309–321. doi: 10.1007/BF01313771. [DOI] [PubMed] [Google Scholar]
  22. Wagner D. K., Graham B. S., Wright P. F., Walsh E. E., Kim H. W., Reimer C. B., Nelson D. L., Chanock R. M., Murphy B. R. Serum immunoglobulin G antibody subclass responses to respiratory syncytial virus F and G glycoproteins after primary infection. J Clin Microbiol. 1986 Aug;24(2):304–306. doi: 10.1128/jcm.24.2.304-306.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wagner D. K., Graham B. S., Wright P. F., Walsh E. E., Kim H. W., Reimer C. B., Nelson D. L., Chanock R. M., Murphy B. R. Serum immunoglobulin G antibody subclass responses to respiratory syncytial virus F and G glycoproteins after primary infection. J Clin Microbiol. 1986 Aug;24(2):304–306. doi: 10.1128/jcm.24.2.304-306.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wagner D. K., Muelenaer P., Henderson F. W., Snyder M. H., Reimer C. B., Walsh E. E., Anderson L. J., Nelson D. L., Murphy B. R. Serum immunoglobulin G antibody subclass response to respiratory syncytial virus F and G glycoproteins after first, second, and third infections. J Clin Microbiol. 1989 Mar;27(3):589–592. doi: 10.1128/jcm.27.3.589-592.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Westenbrink F., Brinkhof J. M., Straver P. J., Quak J., De Leeuw P. W. Comparison of a newly developed enzyme-linked immunosorbent assay with complement fixation and neutralisation tests for serology of bovine respiratory syncytial virus infections. Res Vet Sci. 1985 May;38(3):334–340. [PubMed] [Google Scholar]
  26. Westenbrink F., Kimman T. G., Brinkhof J. M. Analysis of the antibody response to bovine respiratory syncytial virus proteins in calves. J Gen Virol. 1989 Mar;70(Pt 3):591–601. doi: 10.1099/0022-1317-70-3-591. [DOI] [PubMed] [Google Scholar]
  27. van Zaane D., Ijzerman J. Monoclonal antibodies against bovine immunoglobulins and their use in isotype-specific ELISAs for rotavirus antibody. J Immunol Methods. 1984 Sep 4;72(2):427–441. doi: 10.1016/0022-1759(84)90011-5. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Diagnostic Laboratory Immunology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES