Skip to main content
British Journal of Pharmacology and Chemotherapy logoLink to British Journal of Pharmacology and Chemotherapy
. 1964 Dec;23(3):529–539. doi: 10.1111/j.1476-5381.1964.tb01608.x

Urinary excretion of catechol amines in the rat after their liberation by reserpine or dexamphetamine

A M Biscardi, A Carpi, O A Orsingher
PMCID: PMC1703994  PMID: 14256812

Abstract

Daily urinary excretion of catechol amines in normal rats and in rats from which the adrenal medullae had been removed has been determined by a photofluorimetric method. In both groups, reserpine (2 mg/kg, intraperitoneally) produces: (1) A decrease in the urinary excretion of noradrenaline which persists for more than 3 weeks; this action is not influenced by monoamine oxidase inhibitors and mecamylamine. (2) An increase, within 20 to 68 hr, in the urinary excretion of adrenaline, even though the urine of rats without adrenal medullae does not usually contain adrenaline. These effects are prevented by monoamine oxidase inhibitors and, in the normal animals, are reduced by mecamylamine. In both groups, dexamphetamine (6 mg/kg, intraperitoneally) produces an increase in the excretion of adrenaline and noradrenaline, the adrenaline appearing in the urine of the rats without adrenal medullae within 20 to 44 hr. Mecamylamine prevents the effect of dexamphetamine on the excretion of noradrenaline. Dexamphetamine, administered within a week of reserpine treatment, produces its usual effects on the urinary excretion of catechol amines in normal rats, but has no effect in rats without adrenal medullae. The results are discussed with regard to both the mechanism by which reserpine and dexamphetamine influence the peripheral stores of adrenaline and noradrenaline, and the significance of the adrenal and extra-adrenal chromaffin system.

Full text

PDF
529

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BICKEL M. H., CARPI A., BOVET D. Action of reserpine and monoamine oxidase inhibitors on the urinary excretion of catecholamines in the rat. Helv Physiol Pharmacol Acta. 1961;19:279–284. [PubMed] [Google Scholar]
  2. BURN J. H., RAND M. J. The action of sympathomimetic amines in animals treated with reserpine. J Physiol. 1958 Dec 4;144(2):314–336. doi: 10.1113/jphysiol.1958.sp006104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BURN J. H., RAND M. J. The relation of circulating noradrenaline to the effect of sympathetic stimulation. J Physiol. 1960 Feb;150:295–305. doi: 10.1113/jphysiol.1960.sp006388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CALLINGHAM B. A., MANN M. Depletion and replacement of the adrenaline and noradrenaline contents of the rat adrenal gland, following treatment with reserpine. Br J Pharmacol Chemother. 1962 Feb;18:138–149. doi: 10.1111/j.1476-5381.1962.tb01157.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CARLSSON A., RASMUSSEN E. B., KRIST JANSEN P. The urinary excretion of adrenaline and noradrenaline by schizophrenic patients during reserpine treatment. J Neurochem. 1959 Oct;4:318–320. doi: 10.1111/j.1471-4159.1959.tb13211.x. [DOI] [PubMed] [Google Scholar]
  6. CRAWFORD T. B., LAW W. The urinary excretion of adrenaline and noradrenaline by rats under various experimental conditions. Br J Pharmacol Chemother. 1958 Mar;13(1):35–43. doi: 10.1111/j.1476-5381.1958.tb00187.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DE SCHAEPDRYVER A. F. Differential fluorimetric estimation of adrenaline and noradrenaline in urine. Arch Int Pharmacodyn Ther. 1958 May 1;115(1-2):233–245. [PubMed] [Google Scholar]
  8. DE SCHAEPDRYVER A. F., PREZIOSI P., van der STRICHT Urinary adrenaline and noradrenaline output after medullo-adrenalectomy in dogs. Arch Int Pharmacodyn Ther. 1959 Sep 1;121:468–477. [PubMed] [Google Scholar]
  9. GADDUM J. H., KRIVOY W. A., LAVERTY G. The action of reserpine on the excretion of adrenaline and noradrenaline. J Neurochem. 1958;2(2-3):249–253. doi: 10.1111/j.1471-4159.1958.tb12371.x. [DOI] [PubMed] [Google Scholar]
  10. HAZARD R., BEAUVALLET M., FUGAZZA J., SOLIER M. [Urinary excretion of adrenaline and noradrenaline in the rat subjected to the action of reserpine]. J Physiol (Paris) 1960 Jan-Feb;52:113–114. [PubMed] [Google Scholar]
  11. HERTTING G., POTTER L. T., AXELROD J. Effect of decentralization and ganglionic blocking agents on the spontaneous release of H3-norepinephrine. J Pharmacol Exp Ther. 1962 Jun;136:289–292. [PubMed] [Google Scholar]
  12. IGGO A., VOGT M. Preganglionic sympathetic activity in normal and in reserpine-treated cats. J Physiol. 1960 Jan;150:114–133. doi: 10.1113/jphysiol.1960.sp006377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KUSCHKE H. J., VON DITFURTH H. Die Ausscheidung von Noradrenalin und Adrenalin unter hochdosierter Reserpinbehandlung. Klin Wochenschr. 1958 Aug 15;36(16):773–774. doi: 10.1007/BF01482860. [DOI] [PubMed] [Google Scholar]
  14. LEDUC J. Catecholamine production and release in exposure and acclimation to cold. Acta Physiol Scand Suppl. 1961;183:1–101. [PubMed] [Google Scholar]
  15. MIRKIN B. L. The effect of synaptic blocking agents on reserpine-induced alterations in adrenal medullary and urinary catecholamine levels. J Pharmacol Exp Ther. 1961 Jul;133:34–40. [PubMed] [Google Scholar]
  16. MUSCHOLL E., VOGT M. SECRETORY RESPONSES OF EXTRAMEDULLARY CHROMAFFIN TISSUE. Br J Pharmacol Chemother. 1964 Feb;22:193–203. doi: 10.1111/j.1476-5381.1964.tb01559.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SHORE P. A. Release of serotonin and catecholamines by drugs. Pharmacol Rev. 1962 Dec;14:531–550. [PubMed] [Google Scholar]
  18. SPOEREL W. E., GOWDEY C. W. Cardiovascular effects of pentolinium bitartrate in dogs. Can J Biochem Physiol. 1956 Jul;34(4):747–755. [PubMed] [Google Scholar]
  19. VAN PROOSDIJ-HARTZEMA E. G. Observations à propos de l'action hypertensive de la réserpine. J Physiol (Paris) 1959 May-Jun;51(3):551–552. [PubMed] [Google Scholar]
  20. WEINER N., DRASKOCZY P. R., BURACK W. R. The ability of tyramine to liberate catecholamines in vivo. J Pharmacol Exp Ther. 1962 Jul;137:47–55. [PubMed] [Google Scholar]
  21. von EULER U., LISHAJKO F. The estimation of catechol amines in urine. Acta Physiol Scand. 1959 Mar 31;45:122–132. doi: 10.1111/j.1748-1716.1959.tb01684.x. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology and Chemotherapy are provided here courtesy of The British Pharmacological Society

RESOURCES