Skip to main content
PMC home page
Clinical and Diagnostic Laboratory Immunology logoLink to Clinical and Diagnostic Laboratory Immunology
. 1996 Sep;3(5):584–589. doi: 10.1128/cdli.3.5.584-589.1996

Specificity of monoclonal antibodies elicited by mucosal infection of BALB/c mice with virulent Shigella flexneri 2a.

A B Hartman 1, L L Van de Verg 1, C R Mainhart 1, B D Tall 1, S J Smith-Gill 1
PMCID: PMC170411  PMID: 8877140

Abstract

Protective immunity against shigellosis is thought to be determined by the O-antigen side chains of the lipopolysaccharide (LPS) molecule. To study possible common protective epitopes, monoclonal antibodies reacting with Shigella flexneri 2a LPS were generated from BALB/c mice infected ocularly with the virulent serotype 2a strain S. flexneri 2457T and tested against a panel of S. flexneri LPSs by enzyme-linked immunosorbent and immunoblot assays. Four monoclonal antibodies were identified, all of which showed restricted specificity patterns. Three different patterns of reactivity to LPS possessing the 3,4 group antigen were seen: (i) 2a only, (ii) 2a and 5a, and (iii) 2a, 4a, 5a, and Y. These results have implications for designing a Shigella vaccine that will be protective against related serotypes. Electron microscopy studies showed that the monoclonal antibodies bind to the bacterial surface in a patchy pattern, suggesting their potential use for examining the LPS distribution on the surface of the bacteria.

Full Text

The Full Text of this article is available as a PDF (1,006.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayer M. E. Areas of adhesion between wall and membrane of Escherichia coli. J Gen Microbiol. 1968 Oct;53(3):395–404. doi: 10.1099/00221287-53-3-395. [DOI] [PubMed] [Google Scholar]
  2. Carlin N. I., Bundle D. R., Lindberg A. A. Characterization of five Shigella flexneri variant Y-specific monoclonal antibodies using defined saccharides and glycoconjugate antigens. J Immunol. 1987 Jun 15;138(12):4419–4427. [PubMed] [Google Scholar]
  3. Carlin N. I., Lindberg A. A., Bock K., Bundle D. R. The Shigella flexneri O-antigenic polysaccharide chain. Nature of the biological repeating unit. Eur J Biochem. 1984 Feb 15;139(1):189–194. doi: 10.1111/j.1432-1033.1984.tb07993.x. [DOI] [PubMed] [Google Scholar]
  4. Carlin N. I., Lindberg A. A. Monoclonal antibodies specific for Shigella flexneri lipopolysaccharides: clones binding to type I and type III:6,7,8 antigens, group 6 antigen, and a core epitope. Infect Immun. 1986 Jul;53(1):103–109. doi: 10.1128/iai.53.1.103-109.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carlin N. I., Lindberg A. A. Monoclonal antibodies specific for Shigella flexneri lipopolysaccharides: clones binding to type IV, V, and VI antigens, group 3,4 antigen, and an epitope common to all Shigella flexneri and Shigella dysenteriae type 1 stains. Infect Immun. 1987 Jun;55(6):1412–1420. doi: 10.1128/iai.55.6.1412-1420.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen D., Green M. S., Block C., Slepon R., Lerman Y. Natural immunity to shigellosis in two groups with different previous risks of exposure to Shigella is only partly expressed by serum antibodies to lipopolysaccharide. J Infect Dis. 1992 Apr;165(4):785–787. doi: 10.1093/infdis/165.4.785. [DOI] [PubMed] [Google Scholar]
  7. Formal S. B., Oaks E. V., Olsen R. E., Wingfield-Eggleston M., Snoy P. J., Cogan J. P. Effect of prior infection with virulent Shigella flexneri 2a on the resistance of monkeys to subsequent infection with Shigella sonnei. J Infect Dis. 1991 Sep;164(3):533–537. doi: 10.1093/infdis/164.3.533. [DOI] [PubMed] [Google Scholar]
  8. Goldberg M. B., Sansonetti P. J. Shigella subversion of the cellular cytoskeleton: a strategy for epithelial colonization. Infect Immun. 1993 Dec;61(12):4941–4946. doi: 10.1128/iai.61.12.4941-4946.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hartman A. B., Mallett C. P., Srinivasappa J., Prabhakar B. S., Notkins A. L., Smith-Gill S. J. Organ reactive autoantibodies from non-immunized adult BALB/c mice are polyreactive and express non-biased VH gene usage. Mol Immunol. 1989 Apr;26(4):359–370. doi: 10.1016/0161-5890(89)90125-9. [DOI] [PubMed] [Google Scholar]
  10. Hartman A. B., Powell C. J., Schultz C. L., Oaks E. V., Eckels K. H. Small-animal model to measure efficacy and immunogenicity of Shigella vaccine strains. Infect Immun. 1991 Nov;59(11):4075–4083. doi: 10.1128/iai.59.11.4075-4083.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hartman A. B., Van de Verg L. L., Collins H. H., Jr, Tang D. B., Bendiuk N. O., Taylor D. N., Powell C. J. Local immune response and protection in the guinea pig keratoconjunctivitis model following immunization with Shigella vaccines. Infect Immun. 1994 Feb;62(2):412–420. doi: 10.1128/iai.62.2.412-420.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Herrington D. A., Van de Verg L., Formal S. B., Hale T. L., Tall B. D., Cryz S. J., Tramont E. C., Levine M. M. Studies in volunteers to evaluate candidate Shigella vaccines: further experience with a bivalent Salmonella typhi-Shigella sonnei vaccine and protection conferred by previous Shigella sonnei disease. Vaccine. 1990 Aug;8(4):353–357. doi: 10.1016/0264-410x(90)90094-3. [DOI] [PubMed] [Google Scholar]
  13. Kenne L., Lindberg B., Petersson K., Katzenellenbogen E., Romanowska E. Structural studies of Shigella flexneri O-antigens. Eur J Biochem. 1978 Nov 2;91(1):279–284. doi: 10.1111/j.1432-1033.1978.tb20963.x. [DOI] [PubMed] [Google Scholar]
  14. Murayama S. Y., Sakai T., Makino S., Kurata T., Sasakawa C., Yoshikawa M. The use of mice in the Sereny test as a virulence assay of shigellae and enteroinvasive Escherichia coli. Infect Immun. 1986 Feb;51(2):696–698. doi: 10.1128/iai.51.2.696-698.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Newland J. W., Hale T. L., Formal S. B. Genotypic and phenotypic characterization of an aroD deletion-attenuated Escherichia coli K12-Shigella flexneri hybrid vaccine expressing S. flexneri 2a somatic antigen. Vaccine. 1992;10(11):766–776. doi: 10.1016/0264-410x(92)90512-i. [DOI] [PubMed] [Google Scholar]
  16. Okamura N., Nagai T., Nakaya R., Kondo S., Murakami M., Hisatsune K. HeLa cell invasiveness and O antigen of Shigella flexneri as separate and prerequisite attributes of virulence to evoke keratoconjunctivitis in guinea pigs. Infect Immun. 1983 Feb;39(2):505–513. doi: 10.1128/iai.39.2.505-513.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Okamura N., Nakaya R. Rough mutant of Shigella flexneri 2a that penetrates tissue culture cells but does not evoke keratoconjunctivitis in guinea pigs. Infect Immun. 1977 Jul;17(1):4–8. doi: 10.1128/iai.17.1.4-8.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Phalipon A., Michetti P., Kaufmann M., Cavaillon J. M., Huerre M., Kraehenbuhl J. P., Sansonetti P. J. Protection against invasion of the mouse pulmonary epithelium by a monoclonal IgA directed against Shigella flexneri lipopolysaccharide. Ann N Y Acad Sci. 1994 Aug 15;730:356–358. doi: 10.1111/j.1749-6632.1994.tb44291.x. [DOI] [PubMed] [Google Scholar]
  19. SERENY B. Experimental keratoconjunctivitis shigellosa. Acta Microbiol Acad Sci Hung. 1957;4(4):367–376. [PubMed] [Google Scholar]
  20. Shimada T., Sakazaki R. On the serology of Plesiomonas shigelloides. Jpn J Med Sci Biol. 1978 Apr;31(2):135–142. doi: 10.7883/yoken1952.31.135. [DOI] [PubMed] [Google Scholar]
  21. Smith-Gill S. J., Wilson A. C., Potter M., Prager E. M., Feldmann R. J., Mainhart C. R. Mapping the antigenic epitope for a monoclonal antibody against lysozyme. J Immunol. 1982 Jan;128(1):314–322. [PubMed] [Google Scholar]
  22. Tacket C. O., Binion S. B., Bostwick E., Losonsky G., Roy M. J., Edelman R. Efficacy of bovine milk immunoglobulin concentrate in preventing illness after Shigella flexneri challenge. Am J Trop Med Hyg. 1992 Sep;47(3):276–283. doi: 10.4269/ajtmh.1992.47.276. [DOI] [PubMed] [Google Scholar]
  23. Tall B. D., Nauman R. K. Outer membrane antigens of oral Treponema species. J Med Microbiol. 1994 Jan;40(1):62–69. doi: 10.1099/00222615-40-1-62. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Diagnostic Laboratory Immunology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES