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. 1996 Nov;3(6):628–634. doi: 10.1128/cdli.3.6.628-634.1996

Activity of antibodies against Salmonella dublin, Toxoplasma gondii, or Actinobacillus pleuropneumoniae in sera after treatment with electron beam irradiation or binary ethylenimine.

N C Kyvsgaard 1, P Lind 1, T Preuss 1, S Kamstrup 1, J C Lei 1, H O Bøgh 1, P Nansen 1
PMCID: PMC170422  PMID: 8914750

Abstract

Viral contamination of biological material may constitute a risk when samples are exchanged between countries, and it may be necessary to subject the material to an inactivation treatment. The present study investigated possible adverse effects on antibody activity subsequent to either electron beam irradiation or binary ethylenimine (BEI) treatment. The treatments were performed with sera obtained from pigs or cattle. For each treatment level, the posttreatment activity was plotted against the pretreatment activity, and regression analyses were carried out. The slope of the regression line was used as an estimate for the relative posttreatment activity. For a Toxoplasma gondii indirect enzyme-linked immunosorbent assay (ELISA) and agglutination assay as well as for a Salmonella dublin indirect ELISA, the posttreatment activity was more than 89% of the pretreatment activity when the samples were irradiated in the frozen state (on dry ice) with up to 46. kGy or when they were treated with 5 or 10 mM BEI for up to 48 h. The samples were more sensitive to irradiation in the liquid state. Thus, samples irradiated with 22.6 kGy retained 98% of their activity in the indirect ELISA when they were irradiated in the frozen state on dry ice but only 35% of their activity when they were irradiated in the liquid state at 0 degrees C. The patterns seen in an S. dublin blocking ELISA and an Actinobacillus pleuropneumoniae complement fixation assay differed in that samples with a low level of pretreatment activity were subject to a relatively greater decrease in activity than samples with a high level of pretreatment activity. The complement fixation assay was particularly sensitive to irradiation of serum. It is concluded that serum samples retain sufficient activity by both methods of virus inactivation, especially when used in indirect ELISA or in the T. gondii agglutination assay.

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Selected References

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  1. Bahnemann H. G. Binary ethylenimine as an inactivant for foot-and-mouth disease virus and its application for vaccine production. Arch Virol. 1975;47(1):47–56. doi: 10.1007/BF01315592. [DOI] [PubMed] [Google Scholar]
  2. Bahnemann H. G. Inactivation of viruses in serum with binary ethyleneimine. J Clin Microbiol. 1976 Feb;3(2):209–210. doi: 10.1128/jcm.3.2.209-210.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blackburn N. K., Besselaar T. G. A study of the effect of chemical inactivants on the epitopes of Rift Valley fever virus glycoproteins using monoclonal antibodies. J Virol Methods. 1991 Aug;33(3):367–374. doi: 10.1016/0166-0934(91)90036-y. [DOI] [PubMed] [Google Scholar]
  4. Brasch A., Huber W. Ultrashort Application Time of Penetrating Electrons: A Tool for Sterilization and Preservation of Food in the Raw State. Science. 1947 Jan 31;105(2718):112–117. doi: 10.1126/science.105.2718.112. [DOI] [PubMed] [Google Scholar]
  5. Dubey J. P., Thulliez P., Weigel R. M., Andrews C. D., Lind P., Powell E. C. Sensitivity and specificity of various serologic tests for detection of Toxoplasma gondii infection in naturally infected sows. Am J Vet Res. 1995 Aug;56(8):1030–1036. [PubMed] [Google Scholar]
  6. Elliott L. H., McCormick J. B., Johnson K. M. Inactivation of Lassa, Marburg, and Ebola viruses by gamma irradiation. J Clin Microbiol. 1982 Oct;16(4):704–708. doi: 10.1128/jcm.16.4.704-708.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HUBER W. Cold sterilization by electron beam as a possible tool for the inactivation of the virus of homologous serum jaundice in plasma. Ann N Y Acad Sci. 1952 Sep;55(3):536–542. doi: 10.1111/j.1749-6632.1952.tb26574.x. [DOI] [PubMed] [Google Scholar]
  8. Hoorfar J., Feld N. C., Schirmer A. L., Bitsch V., Lind P. Serodiagnosis of Salmonella dublin infection in Danish dairy herds using O-antigen based enzyme-linked immunosorbent assay. Can J Vet Res. 1994 Oct;58(4):268–274. [PMC free article] [PubMed] [Google Scholar]
  9. House C., House J. A., Yedloutschnig R. J. Inactivation of viral agents in bovine serum by gamma irradiation. Can J Microbiol. 1990 Oct;36(10):737–740. doi: 10.1139/m90-126. [DOI] [PubMed] [Google Scholar]
  10. King D. J. Evaluation of different methods of inactivation of Newcastle disease virus and avian influenza virus in egg fluids and serum. Avian Dis. 1991 Jul-Sep;35(3):505–514. [PubMed] [Google Scholar]
  11. Pettersen E. K. Experimental toxoplasmosis in mice and rabbits. Virulence and cyst formation of Toxoplasma gondii. Acta Pathol Microbiol Scand B. 1977 Feb;85B(1):95–102. [PubMed] [Google Scholar]
  12. Saliki J. T., Berninger M. L., Torres A., House J. A., Mebus C. A., Dubovi E. J. Effect of gamma irradiation on reactivity of rinderpest virus antigen with bovine immune serum in enzyme-linked immunosorbent assays and virus neutralization and indirect fluorescent-antibody tests. J Clin Microbiol. 1993 Feb;31(2):428–432. doi: 10.1128/jcm.31.2.428-432.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sullivan R., Fassolitis A. C., Larkin E. P., Read R. B., Jr, Peeler J. T. Inactivation of thirty viruses by gamma radiation. Appl Microbiol. 1971 Jul;22(1):61–65. doi: 10.1128/am.22.1.61-65.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sullivan R., Scarpino P. V., Fassolitis A. C., Larkin E. P., Peeler J. T. Gamma radiation inactivation of coxsackievirus B-2. Appl Microbiol. 1973 Jul;26(1):14–17. doi: 10.1128/am.26.1.14-17.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sun I. L., Gustafson D. P., Scherba G. Comparison of pseudorabies virus inactivated by bromo-ethylene-imine, 60Co irradiation, and acridine dye in immune assay systems. J Clin Microbiol. 1978 Nov;8(5):604–611. doi: 10.1128/jcm.8.5.604-611.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Thomas F. C., Davies A. G., Dulac G. C., Willis N. G., Papp-Vid G., Girard A. Gamma ray inactivation of some animal viruses. Can J Comp Med. 1981 Oct;45(4):397–399. [PMC free article] [PubMed] [Google Scholar]
  17. Venkatesan P., Wakelin D. ELISAs for parasitologists: or lies, damned lies and ELISAs. Parasitol Today. 1993 Jun;9(6):228–232. doi: 10.1016/0169-4758(93)90020-g. [DOI] [PubMed] [Google Scholar]

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