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. 1996 Nov;3(6):779–781. doi: 10.1128/cdli.3.6.779-781.1996

A new procedure for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes supernatant.

E M Mascini 1, M A Hazenberg 1, L A Verhage 1, S E Holm 1, J Verhoef 1, H van Dijk 1
PMCID: PMC170447  PMID: 8914775

Abstract

An important role in the pathogenesis of invasive group A streptococcal disease has been ascribed to the production of streptococcal pyrogenic exotoxin A. We present a new technique for the purification of streptococcal pyrogenic exotoxin A from Streptococcus pyogenes NY-5 supernate, which is highly efficient with respect to yield (35%), purity (> or = 99%), and time.

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Selected References

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