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. 1996 Nov;3(6):782–785. doi: 10.1128/cdli.3.6.782-785.1996

Macrofragment localization of the toxin A and toxin B genes of Clostridium difficile.

D A Norwood Jr 1, J A Sands 1
PMCID: PMC170448  PMID: 8914776

Abstract

We report the physical mapping of the toxin A and B genes to the bacterial chromosome of Clostridium difficile ATCC 43594 by pulsed-field gel electrophoresis. Single and double digestions with restriction endonucleases NruI and SacII allowed localization of the toxin genes to a specific 577-kb fragment and estimation of genome size to be approximately 3.8 megabases. This effort represents the initial step in the construction of a physical map of the whole genome.

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Selected References

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