Abstract
Growth characteristics and cell properties of Aureobasidium (Pullularia) pullulans were studied. The organism grew well on an acid hydrolysate of ryegrass straw over a wide range of pH and temperature. The optimum temperature and pH for the growth of the organism were 32 degrees C and 5.5, respectively. A cell yield of 1.5 g/liter of straw hydrolysate was obtained. The dried cell mass contained 42.6% crude protein, 0.4% crude fat, and 6.4% nucleic acids. The essential amino acid profile of the microbial protein was comparable to that of Candida utilis. A rat feeding study indicated that the A. pullulans cells were not toxic and that the feed intake, weight gain, and protein efficiency ratio values were superior to those obtained with C. utilis. Once the question of mathogenicity is resolved, A. pullulans could be useful for production of single-cell protein from cellulosic wastes.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COOKE W. B. An ecological life history of Aureobasidium pullulans (De Bary) Arnaud. Mycopathol Mycol Appl. 1959 Dec 30;12:1–45. doi: 10.1007/BF02118435. [DOI] [PubMed] [Google Scholar]
- Peitersen N. Cellulase and protein production from mixed cultures of Trichoderma viride and a yeast. Biotechnol Bioeng. 1975 Sep;17(9):1291–1299. doi: 10.1002/bit.260170904. [DOI] [PubMed] [Google Scholar]
- Wickerham L. J., Kurtzman C. P. Synergistic color variants of Aureobasidium pullulans. Mycologia. 1975 Mar-Apr;67(2):342–361. [PubMed] [Google Scholar]
