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. 1997 Mar;4(2):168–172. doi: 10.1128/cdli.4.2.168-172.1997

Characterization of specific immunoglobulin G (IgG) and its subclasses (IgG1 and IgG2) against the 23-valent pneumococcal vaccine in a healthy adult population: proposal for response criteria.

M J Rodrigo 1, M Miravitlles 1, M J Cruz 1, J de Gracia 1, M Vendrell 1, C Pascual 1, F Morell 1
PMCID: PMC170496  PMID: 9067650

Abstract

The aim of the study was to standardize an enzyme-linked immunosorbent assay (ELISA) method for the quantification of immunoglobulin G (IgG) and its subclasses (IgG1 and IgG2) against the 23-valent pneumococcal vaccine and to establish the criteria for a normal response to the vaccine. Forty healthy individuals (20 women and 20 men; mean age, 29 years) were studied. All were vaccinated with the 23-valent pneumococcal vaccine; blood samples were drawn just prior to and 3 weeks after immunization. Quantification of specific IgG and its subclasses was performed by an ELISA with the vaccine as the antigen. The linearity of the ELISA method was demonstrated by the similar slopes of the linear regression lines generated from the titration of sera with different antibody concentrations. The specificity of the antibodies against the vaccine was demonstrated by (i) an absorption test with pneumococcal vaccine, (ii) a cross-reactivity experiment with Haemophilus influenzae type b polysaccharide, and (iii) affinity chromatography with protein A-Sepharose. Response to the vaccine was defined by using the lower level of the 90% probability interval (one-tailed) for postimmunization-specific IgG, IgG1, and IgG2. By using this cutoff, responders were considered to be those with an absolute increase in antibody titers higher than 395 arbitrary units/ml for IgG, 0.350 A450 units for IgG1, and 0.314 A450 units for IgG2. Overall, 20 (50%) subjects had IgG, IgG1, and IgG2 responses, 9 (22.5%) had IgG and IgG2 responses, 4 (10%) had IgG1 responses, 3 (7.5%) had IgG and IgG1 responses, and 4 (10%) were nonresponders. Ninety percent of our population responded to the 23-valent pneumococcal vaccine. Up to 10% of healthy individuals may respond to an IgG subclass without significant increases in total IgG titers. The ELISA method that is described may be useful for evaluating the specific antibody response against polysaccharides.

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Selected References

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  1. Alonso De Velasco E., Dekker B. A., Verheul A. F., Feldman R. G., Verhoef J., Snippe H. Anti-polysaccharide immunoglobulin isotype levels and opsonic activity of antisera: relationships with protection against Streptococcus pneumoniae infection in mice. J Infect Dis. 1995 Aug;172(2):562–565. doi: 10.1093/infdis/172.2.562. [DOI] [PubMed] [Google Scholar]
  2. Ambrosino D. M., Siber G. R., Chilmonczyk B. A., Jernberg J. B., Finberg R. W. An immunodeficiency characterized by impaired antibody responses to polysaccharides. N Engl J Med. 1987 Mar 26;316(13):790–793. doi: 10.1056/NEJM198703263161306. [DOI] [PubMed] [Google Scholar]
  3. Barra A., Schulz D., Aucouturier P., Preud'homme J. L. Measurement of anti-Haemophilus influenzae type b capsular polysaccharide antibodies by ELISA. J Immunol Methods. 1988 Nov 25;115(1):111–117. doi: 10.1016/0022-1759(88)90317-1. [DOI] [PubMed] [Google Scholar]
  4. Barrett D. J., Ayoub E. M. IgG2 subclass restriction of antibody to pneumococcal polysaccharides. Clin Exp Immunol. 1986 Jan;63(1):127–134. [PMC free article] [PubMed] [Google Scholar]
  5. Chudwin D. S., Artrip S. G., Schiffman G. Immunoglobulin G class and subclass antibodies to pneumococcal capsular polysaccharides. Clin Immunol Immunopathol. 1987 Jul;44(1):114–121. doi: 10.1016/0090-1229(87)90057-2. [DOI] [PubMed] [Google Scholar]
  6. Cryz S. J., Jr, Fürer E., Fredeking T., Cross A. S., Sadoff J. C., Que J. U. Effect of race and blood group on the immune response to bacterial polysaccharide and conjugate vaccines. Lancet. 1989 Dec 23;2(8678-8679):1533–1534. doi: 10.1016/s0140-6736(89)92985-1. [DOI] [PubMed] [Google Scholar]
  7. Cunningham-Rundles C. Clinical and immunologic analyses of 103 patients with common variable immunodeficiency. J Clin Immunol. 1989 Jan;9(1):22–33. doi: 10.1007/BF00917124. [DOI] [PubMed] [Google Scholar]
  8. De Gracia J., Rodrigo M. J., Morell F., Vendrell M., Miravitlles M., Cruz M. J., Codina R., Bofill J. M. IgG subclass deficiencies associated with bronchiectasis. Am J Respir Crit Care Med. 1996 Feb;153(2):650–655. doi: 10.1164/ajrccm.153.2.8564113. [DOI] [PubMed] [Google Scholar]
  9. Kroon F. P., van Dissel J. T., de Jong J. C., van Furth R. Antibody response to influenza, tetanus and pneumococcal vaccines in HIV-seropositive individuals in relation to the number of CD4+ lymphocytes. AIDS. 1994 Apr;8(4):469–476. doi: 10.1097/00002030-199404000-00008. [DOI] [PubMed] [Google Scholar]
  10. Landesman S. H., Schiffman G. Assessment of the antibody response to pneumococcal vaccine in high-risk populations. Rev Infect Dis. 1981 Mar-Apr;3 (Suppl):S184–S197. doi: 10.1093/clinids/3.supplement_1.s184. [DOI] [PubMed] [Google Scholar]
  11. Leibl H., Erber W., Eibl M. M., Mannhalter J. W. Separation of polysaccharide-specific human immunoglobulin G subclasses using a protein A superose column with a pH gradient elution system. J Chromatogr. 1993 Jun 4;639(1):51–56. doi: 10.1016/0021-9673(93)83087-9. [DOI] [PubMed] [Google Scholar]
  12. Mascart-Lemone F., Gérard M., Libin M., Crusiaux A., Franchioly P., Lambrechts A., Goldman M., Clumeck N. Differential effect of human immunodeficiency virus infection on the IgA and IgG antibody responses to pneumococcal vaccine. J Infect Dis. 1995 Nov;172(5):1253–1260. doi: 10.1093/infdis/172.5.1253. [DOI] [PubMed] [Google Scholar]
  13. Metzger W. J., Butler J. E., Swanson P., Reinders E., Richerson H. B. Amplification of the enzyme-linked immunosorbent assay for measuring allergen-specific IgE and IgG antibody. Clin Allergy. 1981 Nov;11(6):523–531. doi: 10.1111/j.1365-2222.1981.tb02170.x. [DOI] [PubMed] [Google Scholar]
  14. Musher D. M., Luchi M. J., Watson D. A., Hamilton R., Baughn R. E. Pneumococcal polysaccharide vaccine in young adults and older bronchitics: determination of IgG responses by ELISA and the effect of adsorption of serum with non-type-specific cell wall polysaccharide. J Infect Dis. 1990 Apr;161(4):728–735. doi: 10.1093/infdis/161.4.728. [DOI] [PubMed] [Google Scholar]
  15. Oldfield S., Jenkins S., Yeoman H., Gray D., MacLennan I. C. Class and subclass anti-pneumococcal antibody responses in splenectomized patients. Clin Exp Immunol. 1985 Sep;61(3):664–673. [PMC free article] [PubMed] [Google Scholar]
  16. Rodrigo M. J., Codina R., de Gracia J., Morell F., Pascual C. Valores normales de las subclases de la inmunoglobulina G en una población de adultos. Su importancia en el estudio de los déficit de las mismas. Med Clin (Barc) 1992 Feb 8;98(5):166–170. [PubMed] [Google Scholar]
  17. Rodriguez-Barradas M. C., Musher D. M., Lahart C., Lacke C., Groover J., Watson D., Baughn R., Cate T., Crofoot G. Antibody to capsular polysaccharides of Streptococcus pneumoniae after vaccination of human immunodeficiency virus-infected subjects with 23-valent pneumococcal vaccine. J Infect Dis. 1992 Mar;165(3):553–556. doi: 10.1093/infdis/165.3.553. [DOI] [PubMed] [Google Scholar]
  18. Rynnel-Dagö B., Freijd A., Prellner K. Antibody activity of IgG subclasses against pneumococcal polysaccharides after vaccination. Am J Otolaryngol. 1985 Jul-Aug;6(4):275–279. doi: 10.1016/s0196-0709(85)80055-7. [DOI] [PubMed] [Google Scholar]
  19. Schaffer F. M., Monteiro R. C., Volanakis J. E., Cooper M. D. IgA deficiency. Immunodefic Rev. 1991;3(1):15–44. [PubMed] [Google Scholar]
  20. Siber G. R. Pneumococcal disease: prospects for a new generation of vaccines. Science. 1994 Sep 2;265(5177):1385–1387. doi: 10.1126/science.8073278. [DOI] [PubMed] [Google Scholar]
  21. Siber G. R., Priehs C., Madore D. V. Standardization of antibody assays for measuring the response to pneumococcal infection and immunization. Pediatr Infect Dis J. 1989 Jan;8(1 Suppl):S84–S91. [PubMed] [Google Scholar]
  22. Siber G. R., Schur P. H., Aisenberg A. C., Weitzman S. A., Schiffman G. Correlation between serum IgG-2 concentrations and the antibody response to bacterial polysaccharide antigens. N Engl J Med. 1980 Jul 24;303(4):178–182. doi: 10.1056/NEJM198007243030402. [DOI] [PubMed] [Google Scholar]
  23. Sneller M. C., Strober W., Eisenstein E., Jaffe J. S., Cunningham-Rundles C. NIH conference. New insights into common variable immunodeficiency. Ann Intern Med. 1993 May 1;118(9):720–730. doi: 10.7326/0003-4819-118-9-199305010-00011. [DOI] [PubMed] [Google Scholar]
  24. Umetsu D. T., Ambrosino D. M., Quinti I., Siber G. R., Geha R. S. Recurrent sinopulmonary infection and impaired antibody response to bacterial capsular polysaccharide antigen in children with selective IgG-subclass deficiency. N Engl J Med. 1985 Nov 14;313(20):1247–1251. doi: 10.1056/NEJM198511143132002. [DOI] [PubMed] [Google Scholar]
  25. Weiss P. J., Wallace M. R., Oldfield E. C., 3rd, O'Brien J., Janoff E. N. Response of recent human immunodeficiency virus seroconverters to the pneumococcal polysaccharide vaccine and Haemophilus influenzae type b conjugate vaccine. J Infect Dis. 1995 May;171(5):1217–1222. doi: 10.1093/infdis/171.5.1217. [DOI] [PubMed] [Google Scholar]
  26. de Gracia J., Miravitlles M., Vendrell M., Ròdrigo M. J., Codina R., Morell F. Estudio de las subclases de la IgG en pacientes con déficit de IgA sintomáticos. Med Clin (Barc) 1995 May 20;104(19):728–731. [PubMed] [Google Scholar]

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