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Clinical and Diagnostic Laboratory Immunology logoLink to Clinical and Diagnostic Laboratory Immunology
. 1997 May;4(3):339–344. doi: 10.1128/cdli.4.3.339-344.1997

High prevalence of Borna disease virus infection in healthy sheep in Japan.

K Hagiwara 1, S Kawamoto 1, H Takahashi 1, Y Nakamura 1, T Nakaya 1, T Hiramune 1, C Ishihara 1, K Ikuta 1
PMCID: PMC170529  PMID: 9144374

Abstract

Previous seroepidemiological and molecular epidemiological studies of Borna disease virus (BDV) showed considerably high rates of infection in horses, cattle, cats, and humans in Hokkaido, Japan. Here, we further demonstrate high rates of specific antibodies to BDV and BDV RNA in peripheral blood mononuclear cells (PBMCs) from healthy sheep bred on the same island. The BDV prevalences by immunoblotting and/or reverse transcriptase PCR were 0% (0 of 19) in newborns (<1 month old), 51.7% (15 of 29) in lambs (1 to 6 months old), and 36.7% (11 of 30) in adults (>2 years old). Among animals positive for BDV, 60% of lambs and 45.5% of adults contained BDV RNA in PBMCs while 46.7% of lambs and 90.9% of adults contained specific antibodies to BDV. Thus, it is suggested that virus replication in the blood, as observed in lambs, is usually reduced in adulthood by raising immune responses to BDV.

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Selected References

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  1. Amsterdam J. D., Winokur A., Dyson W., Herzog S., Gonzalez F., Rott R., Koprowski H. Borna disease virus. A possible etiologic factor in human affective disorders? Arch Gen Psychiatry. 1985 Nov;42(11):1093–1096. doi: 10.1001/archpsyc.1985.01790340077011. [DOI] [PubMed] [Google Scholar]
  2. Auwanit W., Ayuthaya P. I., Nakaya T., Fujiwara S., Kurata T., Yamanishi K., Ikuta K. Unusually high seroprevalence of Borna disease virus in clade E human immunodeficiency virus type 1-infected patients with sexually transmitted diseases in Thailand. Clin Diagn Lab Immunol. 1996 Sep;3(5):590–593. doi: 10.1128/cdli.3.5.590-593.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bahmani M. K., Nowrouzian I., Nakaya T., Nakamura Y., Hagiwara K., Takahashi H., Rad M. A., Ikuta K. Varied prevalence of Borna disease virus infection in Arabic, thoroughbred and their cross-bred horses in Iran. Virus Res. 1996 Nov;45(1):1–13. doi: 10.1016/0168-1702(96)01355-x. [DOI] [PubMed] [Google Scholar]
  4. Binz T., Lebelt J., Niemann H., Hagenau K. Sequence analyses of the p24 gene of Borna disease virus in naturally infected horse, donkey and sheep. Virus Res. 1994 Dec;34(3):281–289. doi: 10.1016/0168-1702(94)90128-7. [DOI] [PubMed] [Google Scholar]
  5. Bode L., Ferszt R., Czech G. Borna disease virus infection and affective disorders in man. Arch Virol Suppl. 1993;7:159–167. doi: 10.1007/978-3-7091-9300-6_13. [DOI] [PubMed] [Google Scholar]
  6. Bode L., Riegel S., Lange W., Ludwig H. Human infections with Borna disease virus: seroprevalence in patients with chronic diseases and healthy individuals. J Med Virol. 1992 Apr;36(4):309–315. doi: 10.1002/jmv.1890360414. [DOI] [PubMed] [Google Scholar]
  7. Bode L., Riegel S., Ludwig H., Amsterdam J. D., Lange W., Koprowski H. Borna disease virus-specific antibodies in patients with HIV infection and with mental disorders. Lancet. 1988 Sep 17;2(8612):689–689. doi: 10.1016/s0140-6736(88)90505-3. [DOI] [PubMed] [Google Scholar]
  8. Bode L., Steinbach F., Ludwig H. A novel marker for Borna disease virus infection. Lancet. 1994 Jan 29;343(8892):297–298. doi: 10.1016/s0140-6736(94)91147-9. [DOI] [PubMed] [Google Scholar]
  9. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  10. Cubitt B., Oldstone C., de la Torre J. C. Sequence and genome organization of Borna disease virus. J Virol. 1994 Mar;68(3):1382–1396. doi: 10.1128/jvi.68.3.1382-1396.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. De Rossi A., Ometto L., Mammano F., Zanotto C., Giaquinto C., Chieco-Bianchi L. Vertical transmission of HIV-1: lack of detectable virus in peripheral blood cells of infected children at birth. AIDS. 1992 Oct;6(10):1117–1120. [PubMed] [Google Scholar]
  12. Ehrnst A., Lindgren S., Dictor M., Johansson B., Sönnerborg A., Czajkowski J., Sundin G., Bohlin A. B. HIV in pregnant women and their offspring: evidence for late transmission. Lancet. 1991 Jul 27;338(8761):203–207. doi: 10.1016/0140-6736(91)90347-r. [DOI] [PubMed] [Google Scholar]
  13. Fu Z. F., Amsterdam J. D., Kao M., Shankar V., Koprowski H., Dietzschold B. Detection of Borna disease virus-reactive antibodies from patients with affective disorders by western immunoblot technique. J Affect Disord. 1993 Jan;27(1):61–68. doi: 10.1016/0165-0327(93)90098-5. [DOI] [PubMed] [Google Scholar]
  14. Hagiwara K., Nakaya T., Nakamura Y., Asahi S., Takahashi H., Ishihara C., Ikuta K. Borna disease virus RNA in peripheral blood mononuclear cells obtained from healthy dairy cattle. Med Microbiol Immunol. 1996 Nov;185(3):145–151. doi: 10.1007/s004300050024. [DOI] [PubMed] [Google Scholar]
  15. Herzog S., Rott R. Replication of Borna disease virus in cell cultures. Med Microbiol Immunol. 1980;168(3):153–158. doi: 10.1007/BF02122849. [DOI] [PubMed] [Google Scholar]
  16. Kao M., Hamir A. N., Rupprecht C. E., Fu Z. F., Shankar V., Koprowski H., Dietzschold B. Detection of antibodies against Borna disease virus in sera and cerebrospinal fluid of horses in the USA. Vet Rec. 1993 Mar 6;132(10):241–244. doi: 10.1136/vr.132.10.241. [DOI] [PubMed] [Google Scholar]
  17. Kishi M., Arimura Y., Ikuta K., Shoya Y., Lai P. K., Kakinuma M. Sequence variability of Borna disease virus open reading frame II found in human peripheral blood mononuclear cells. J Virol. 1996 Jan;70(1):635–640. doi: 10.1128/jvi.70.1.635-640.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kishi M., Nakaya T., Nakamura Y., Kakinuma M., Takahashi T. A., Sekiguchi S., Uchikawa M., Tadokoro K., Ikeda K., Ikuta K. Prevalence of Borna disease virus RNA in peripheral blood mononuclear cells from blood donors. Med Microbiol Immunol. 1995 Oct;184(3):135–138. doi: 10.1007/BF00224350. [DOI] [PubMed] [Google Scholar]
  19. Kishi M., Nakaya T., Nakamura Y., Zhong Q., Ikeda K., Senjo M., Kakinuma M., Kato S., Ikuta K. Demonstration of human Borna disease virus RNA in human peripheral blood mononuclear cells. FEBS Lett. 1995 May 15;364(3):293–297. doi: 10.1016/0014-5793(95)00406-y. [DOI] [PubMed] [Google Scholar]
  20. Ludwig H., Bode L., Gosztonyi G. Borna disease: a persistent virus infection of the central nervous system. Prog Med Virol. 1988;35:107–151. [PubMed] [Google Scholar]
  21. Ludwig H., Kraft W., Kao M., Gosztonyi G., Dahme E., Krey H. Borna-Virus-Infektion (Borna-Krankheit) bei natürlich und experimentell infizierten Tieren: ihre Bedeutung für Forschung und Praxis. Tierarztl Prax. 1985;13(4):421–453. [PubMed] [Google Scholar]
  22. Luzuriaga K., McQuilken P., Alimenti A., Somasundaran M., Hesselton R., Sullivan J. L. Early viremia and immune responses in vertical human immunodeficiency virus type 1 infection. J Infect Dis. 1993 May;167(5):1008–1013. doi: 10.1093/infdis/167.5.1008. [DOI] [PubMed] [Google Scholar]
  23. Nakamura Y., Asahi S., Nakaya T., Bahmani M. K., Saitoh S., Yasui K., Mayama H., Hagiwara K., Ishihara C., Ikuta K. Demonstration of borna disease virus RNA in peripheral blood mononuclear cells derived from domestic cats in Japan. J Clin Microbiol. 1996 Jan;34(1):188–191. doi: 10.1128/jcm.34.1.188-191.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Nakamura Y., Kishi M., Nakaya T., Asahi S., Tanaka H., Sentsui H., Ikeda K., Ikuta K. Demonstration of Borna disease virus RNA in peripheral blood mononuclear cells from healthy horses in Japan. Vaccine. 1995 Aug;13(12):1076–1079. doi: 10.1016/0264-410x(95)00050-b. [DOI] [PubMed] [Google Scholar]
  25. Nakaya T., Takahashi H., Nakamura Y., Asahi S., Tobiume M., Kuratsune H., Kitani T., Yamanishi K., Ikuta K. Demonstration of Borna disease virus RNA in peripheral blood mononuclear cells derived from Japanese patients with chronic fatigue syndrome. FEBS Lett. 1996 Jan 8;378(2):145–149. doi: 10.1016/0014-5793(95)01439-x. [DOI] [PubMed] [Google Scholar]
  26. Richt J. A., Herzog S., Pyper J., Clements J. E., Narayan O., Bechter K., Rott R. Borna disease virus: nature of the etiologic agent and significance of infection in man. Arch Virol Suppl. 1993;7:101–109. doi: 10.1007/978-3-7091-9300-6_9. [DOI] [PubMed] [Google Scholar]
  27. Rott R., Herzog S., Bechter K., Frese K. Borna disease, a possible hazard for man? Arch Virol. 1991;118(3-4):143–149. doi: 10.1007/BF01314025. [DOI] [PubMed] [Google Scholar]
  28. Rott R., Herzog S., Fleischer B., Winokur A., Amsterdam J., Dyson W., Koprowski H. Detection of serum antibodies to Borna disease virus in patients with psychiatric disorders. Science. 1985 May 10;228(4700):755–756. doi: 10.1126/science.3922055. [DOI] [PubMed] [Google Scholar]
  29. Sauder C., Müller A., Cubitt B., Mayer J., Steinmetz J., Trabert W., Ziegler B., Wanke K., Mueller-Lantzsch N., de la Torre J. C. Detection of Borna disease virus (BDV) antibodies and BDV RNA in psychiatric patients: evidence for high sequence conservation of human blood-derived BDV RNA. J Virol. 1996 Nov;70(11):7713–7724. doi: 10.1128/jvi.70.11.7713-7724.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Schneemann A., Schneider P. A., Lamb R. A., Lipkin W. I. The remarkable coding strategy of borna disease virus: a new member of the nonsegmented negative strand RNA viruses. Virology. 1995 Jun 20;210(1):1–8. doi: 10.1006/viro.1995.1311. [DOI] [PubMed] [Google Scholar]
  31. Schneider P. A., Briese T., Zimmermann W., Ludwig H., Lipkin W. I. Sequence conservation in field and experimental isolates of Borna disease virus. J Virol. 1994 Jan;68(1):63–68. doi: 10.1128/jvi.68.1.63-68.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. VandeWoude S., Richt J. A., Zink M. C., Rott R., Narayan O., Clements J. E. A borna virus cDNA encoding a protein recognized by antibodies in humans with behavioral diseases. Science. 1990 Nov 30;250(4985):1278–1281. doi: 10.1126/science.2244211. [DOI] [PubMed] [Google Scholar]
  33. Waelchli R. O., Ehrensperger F., Metzler A., Winder C. Borna disease in a sheep. Vet Rec. 1985 Nov 9;117(19):499–500. doi: 10.1136/vr.117.19.499. [DOI] [PubMed] [Google Scholar]
  34. Waltrip R. W., 2nd, Buchanan R. W., Summerfelt A., Breier A., Carpenter W. T., Jr, Bryant N. L., Rubin S. A., Carbone K. M. Borna disease virus and schizophrenia. Psychiatry Res. 1995 Jan 31;56(1):33–44. doi: 10.1016/0165-1781(94)02600-n. [DOI] [PubMed] [Google Scholar]
  35. de la Torre J. C. Molecular biology of borna disease virus: prototype of a new group of animal viruses. J Virol. 1994 Dec;68(12):7669–7675. doi: 10.1128/jvi.68.12.7669-7675.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

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