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. 1997 May;4(3):345–351. doi: 10.1128/cdli.4.3.345-351.1997

Measurement of antibodies against meningococcal capsular polysaccharides B and C in enzyme-linked immunosorbent assays: towards an improved surveillance of meningococcal disease.

J Andersen 1, L Berthelsen 1, I Lind 1
PMCID: PMC170530  PMID: 9144375

Abstract

In order to improve the surveillance of serogroup B and C meningococcal diseases, enzyme-linked immunosorbent assays (ELISAs) specific for anti-B immunoglobulin M (IgM) and anti-C IgM and IgG antibodies were developed. The tests were evaluated by using paired sera from 122 patients with and 101 patients without laboratory evidence of meningococcal disease. Fifty-three of 67 patients (79%) with culture-confirmed serogroup B disease had an anti-B IgM antibody response; anti-B IgM levels waned rapidly in children < or = 4 years of age. Twenty-four of 25 patients (96%) with culture-confirmed serogroup C disease had an anti-C IgM and/or IgG antibody response (IgM, 92%; IgG, 68%). In patients without evidence of meningococcal disease, 19% of children < or = 4 years of age and 69% of those > 4 years of age had intermediate anti-B IgM titers. In contrast, only 1 and 5% of these patients had intermediate titers of anti-C IgM and anti-C IgG, respectively. The ELISAs were shown to be powerful tools for discriminating between serogroup B and C diseases in 96 to 100% of culture-confirmed cases. For 90% of patients with culture-negative meningococcal disease, a serogroup-specific diagnosis could be established by examination of paired sera in the ELISAs. As serogroup B and C meningococci account for practically all cases of meningococcal disease in industrialized countries, the availability of these tests may improve surveillance and prevention.

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Selected References

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  1. Arakere G., Frasch C. E. Specificity of antibodies to O-acetyl-positive and O-acetyl-negative group C meningococcal polysaccharides in sera from vaccinees and carriers. Infect Immun. 1991 Dec;59(12):4349–4356. doi: 10.1128/iai.59.12.4349-4356.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Begg N. Reducing mortality from meningococcal disease. BMJ. 1992 Jul 18;305(6846):133–134. doi: 10.1136/bmj.305.6846.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brandt B. L., Wyle F. A., Artenstein M. S. A radioactive antigen-binding assay for Neisseria meningitidis polysaccharide antibody. J Immunol. 1972 Apr;108(4):913–920. [PubMed] [Google Scholar]
  4. Carlone G. M., Frasch C. E., Siber G. R., Quataert S., Gheesling L. L., Turner S. H., Plikaytis B. D., Helsel L. O., DeWitt W. E., Bibb W. F. Multicenter comparison of levels of antibody to the Neisseria meningitidis group A capsular polysaccharide measured by using an enzyme-linked immunosorbent assay. J Clin Microbiol. 1992 Jan;30(1):154–159. doi: 10.1128/jcm.30.1.154-159.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Devi S. J., Robbins J. B., Schneerson R. Antibodies to poly[(2----8)-alpha-N-acetylneuraminic acid] and poly[(2----9)-alpha-N-acetylneuraminic acid] are elicited by immunization of mice with Escherichia coli K92 conjugates: potential vaccines for groups B and C meningococci and E. coli K1. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7175–7179. doi: 10.1073/pnas.88.16.7175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Diaz Romero J., Outschoorn I. M. Current status of meningococcal group B vaccine candidates: capsular or noncapsular? Clin Microbiol Rev. 1994 Oct;7(4):559–575. doi: 10.1128/cmr.7.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Diaz Romero J., Outschoorn I. Selective biotinylation of Neisseria meningitidis group B capsular polysaccharide and application in an improved ELISA for the detection of specific antibodies. J Immunol Methods. 1993 Mar 15;160(1):35–47. doi: 10.1016/0022-1759(93)90006-s. [DOI] [PubMed] [Google Scholar]
  8. Edwards E. A., Devine L. F., Sengbusch G. H., Ward H. W. Immunological investigations of meningococcal disease. III. Brevity of group C acquisition prior to disease occurrence. Scand J Infect Dis. 1977;9(2):105–110. doi: 10.3109/inf.1977.9.issue-2.09. [DOI] [PubMed] [Google Scholar]
  9. Finne J., Leinonen M., Mäkelä P. H. Antigenic similarities between brain components and bacteria causing meningitis. Implications for vaccine development and pathogenesis. Lancet. 1983 Aug 13;2(8346):355–357. doi: 10.1016/s0140-6736(83)90340-9. [DOI] [PubMed] [Google Scholar]
  10. Gheesling L. L., Carlone G. M., Pais L. B., Holder P. F., Maslanka S. E., Plikaytis B. D., Achtman M., Densen P., Frasch C. E., Käyhty H. Multicenter comparison of Neisseria meningitidis serogroup C anti-capsular polysaccharide antibody levels measured by a standardized enzyme-linked immunosorbent assay. J Clin Microbiol. 1994 Jun;32(6):1475–1482. doi: 10.1128/jcm.32.6.1475-1482.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goldschneider I., Gotschlich E. C., Artenstein M. S. Human immunity to the meningococcus. II. Development of natural immunity. J Exp Med. 1969 Jun 1;129(6):1327–1348. doi: 10.1084/jem.129.6.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Griffiss J. M., Brandt B. L., Broud D. D., Goroff D. K., Baker C. J. Immune response of infants and children to disseminated infections with Neisseria meningitidis. J Infect Dis. 1984 Jul;150(1):71–79. doi: 10.1093/infdis/150.1.71. [DOI] [PubMed] [Google Scholar]
  13. Høiby E. A., Bjune G., Frøholm L. O., Eng J., Halstensen A., Rosenqvist E., Rønnild E., Wedege E. The Norwegian meningococcal serogroup B outer membrane vesicle vaccine protection trials: case tracing, meningococcal antigen detection and serological diagnosis. NIPH Ann. 1991 Dec;14(2):107–123. [PubMed] [Google Scholar]
  14. Jackson L. A., Schuchat A., Reeves M. W., Wenger J. D. Serogroup C meningococcal outbreaks in the United States. An emerging threat. JAMA. 1995 Feb 1;273(5):383–389. [PubMed] [Google Scholar]
  15. Konradsen H. B., Sørensen U. B., Henrichsen J. A modified enzyme-linked immunosorbent assay for measuring type-specific anti-pneumococcal capsular polysaccharide antibodies. J Immunol Methods. 1993 Aug 26;164(1):13–20. doi: 10.1016/0022-1759(93)90270-h. [DOI] [PubMed] [Google Scholar]
  16. Käyhty H., Jousimies-Somer H., Peltola H., Mäketä P. H. Antibody response to capsular polysaccharides of groups A and C neisseria meningitidis and Haemophilus influenzae type b during bacteremic disease. J Infect Dis. 1981 Jan;143(1):32–41. doi: 10.1093/infdis/143.1.32. [DOI] [PubMed] [Google Scholar]
  17. Leinonen M., Frasch C. E. Class-specific antibody response to group B Neisseria meningitidis capsular polysaccharide: use of polylysine precoating in an enzyme-linked immunosorbent assay. Infect Immun. 1982 Dec;38(3):1203–1207. doi: 10.1128/iai.38.3.1203-1207.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Salih M. A., Fredlund H., Hugosson S., Bodin L., Olcén P. Different seroprevalences of antibodies against Neisseria meningitidis serogroup A and Haemophilus influenzae type b in Sudanese and Swedish children. Epidemiol Infect. 1993 Apr;110(2):307–316. doi: 10.1017/s0950268800068242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Samuelsson S., Gustavsen S., Rønne T. Epidemiology of meningococcal disease in Denmark 1980-1988. Scand J Infect Dis. 1991;23(6):723–730. doi: 10.3109/00365549109024300. [DOI] [PubMed] [Google Scholar]
  20. Schwartz B., Moore P. S., Broome C. V. Global epidemiology of meningococcal disease. Clin Microbiol Rev. 1989 Apr;2 (Suppl):S118–S124. doi: 10.1128/cmr.2.suppl.s118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sippel J. E., Girgis N. I. Detection of IgM antibodies to Neisseria meningitidis group A polysaccharide in meningitis patients by direct and antibody capture enzyme-linked immunosorbent assays. Am J Trop Med Hyg. 1991 Dec;45(6):676–682. doi: 10.4269/ajtmh.1991.45.676. [DOI] [PubMed] [Google Scholar]
  22. Sørensen H. T., Møller-Petersen J., Krarup H. B., Pedersen H., Hansen H., Hamburger H. Early treatment of meningococcal disease. BMJ. 1992 Sep 26;305(6856):774–774. doi: 10.1136/bmj.305.6856.774-a. [DOI] [PMC free article] [PubMed] [Google Scholar]

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