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. 1997 Sep;4(5):587–591. doi: 10.1128/cdli.4.5.587-591.1997

Field evaluation of an immunoglobulin G anti-F1 enzyme-linked immunosorbent assay for serodiagnosis of human plague in Madagascar.

B Rasoamanana 1, F Leroy 1, P Boisier 1, M Rasolomaharo 1, P Buchy 1, E Carniel 1, S Chanteau 1
PMCID: PMC170602  PMID: 9302210

Abstract

Bacteriological isolation of Yersinia pestis is the reference test for confirming plague infection, but recovery of the pathogen from human samples is usually very poor. When the etiology of the disease cannot be bacteriologically confirmed, it may be useful to possess alternative tests such as detection of specific circulating antibodies to help guide the diagnosis. In the present study, the immunoglobulin G (IgG) anti-F1 enzyme-linked immunosorbent assay (ELISA) has been applied to various human sera to evaluate its large-scale applicability in the high-endemicity plague foci of Madagascar. The sensitivity of the test was found to be 91.4%, and its specificity was 98.5%. The positive and negative predictive values were 96 and 96.6%, respectively. Seroconversion was observed on day 7 after onset of the disease. Patients with a positive ELISA result could be separated into high (82%) and low (18%) IgG anti-F1 responders. Cross-reactions with eight other infectious diseases prevalent in Madagascar were scarce and were found in 1 of 27 Mycobacterium tuberculosis-, 3 of 34 Schistosoma haematobium-, and 1 of 41 Salmonella-infected patients. Finally, the efficiency of the IgG anti-F1 ELISA was evaluated during the Mahajanga, Madagascar, plague outbreak of 1995. When the number of ELISA-positive patients was added to the number of bacteriologically confirmed and probable cases, the number of positive patients was increased by 35%. In conclusion, although it does not replace bacteriology, IgG anti-F1 ELISA is a useful and powerful tool for retrospective diagnosis and epidemiological surveillance of plague outbreaks.

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Selected References

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  1. BAKER E. E., SOMMER H., FOSTER L. E., MEYER E., MEYER K. F. Studies on immunization against plague. I. The isolation and characterization of the soluble antigen of Pasteurella pestis. J Immunol. 1952 Feb;68(2):131–145. [PubMed] [Google Scholar]
  2. Benoit C., Guiyoule A., Carniel E. Sérodiagnostic des infections humaines à Yersinia pathogènes. Presse Med. 1996 Nov 9;25(34):1627–1630. [PubMed] [Google Scholar]
  3. Butler T., Hudson B. W. The serological response to Yersinia pestis infection. Bull World Health Organ. 1977;55(1):39–42. [PMC free article] [PubMed] [Google Scholar]
  4. Cavanaugh D. C., Deoras P. J., Hunter D. H., Marshall J. D., Jr, Do-van-Quy, Rust J. H., Jr, Purnaveja S., Winter P. E. Some observations on the necessity for serological testing of rodent sera for Pasteurella pestis antibody in a plague control programme. Bull World Health Organ. 1970;42(3):451–459. [PMC free article] [PubMed] [Google Scholar]
  5. Cavanaugh D. C., Fortier M. K., Robinson D. M., Williams J. E., Rust J. H., Jr Application of the ELISA technique to problems in the serologic diagnosis of plague. Bull Pan Am Health Organ. 1979;13(4):399–402. [PubMed] [Google Scholar]
  6. Champetier de Ribes G., Rasoamanana B., Randriambelosoa J., Rakoto L. J., Rabescn D., Chanteau S. La peste à Madagascar: données épidémiologiques de 1989 à 1995 et programme national de lutte. Sante. 1997 Jan-Feb;7(1):53–60. [PubMed] [Google Scholar]
  7. Chen T. H., Meyer K. F. An evaluation of Pasteurella pestis fraction-1-specific antibody for the confirmation of plague infections. Bull World Health Organ. 1966;34(6):911–918. [PMC free article] [PubMed] [Google Scholar]
  8. Dodin A., Brygoo E. R. La réaction d'hémagglutination conditionnée dans la peste, chez différents hotes. Rapport entre les anticorps hémagglutinants et précipitants. I. Les polyosides. Ann Inst Pasteur (Paris) 1968 Apr;114(4):452–462. [PubMed] [Google Scholar]
  9. Marshall J. D., Jr, Cavanaugh D. C., Bartelloni P. J., Meyer K. F. Plague immunization. 3. Serologic response to multiple inoculations of vaccine. J Infect Dis. 1974 May;129(Suppl):S26–S29. doi: 10.1093/infdis/129.supplement_1.s26. [DOI] [PubMed] [Google Scholar]
  10. Mavalankar D. V. Indian 'plague' epidemic: unanswered questions and key lessons. J R Soc Med. 1995 Oct;88(10):547–551. [PMC free article] [PubMed] [Google Scholar]
  11. Metz C. E. Basic principles of ROC analysis. Semin Nucl Med. 1978 Oct;8(4):283–298. doi: 10.1016/s0001-2998(78)80014-2. [DOI] [PubMed] [Google Scholar]
  12. Meyer K. F. Effectiveness of live or killed plague vaccines in man. Bull World Health Organ. 1970;42(5):653–666. [PMC free article] [PubMed] [Google Scholar]
  13. Rasolomaharo M., Rasoamanana B., Andrianirina Z., Buchy P., Rakotoarimanana N., Chanteau S. Plague in Majunga, Madagascar. Lancet. 1995 Nov 4;346(8984):1234–1234. doi: 10.1016/s0140-6736(95)92944-4. [DOI] [PubMed] [Google Scholar]
  14. Shepherd A. J., Hummitzsch D. E., Leman P. A., Hartwig E. K. Studies on plague in the eastern Cape Province of South Africa. Trans R Soc Trop Med Hyg. 1983;77(6):800–808. doi: 10.1016/0035-9203(83)90293-6. [DOI] [PubMed] [Google Scholar]
  15. Shepherd A. J., Hummitzsch D. E., Leman P. A., Swanepoel R., Searle L. A. Comparative tests for detection of plague antigen and antibody in experimentally infected wild rodents. J Clin Microbiol. 1986 Dec;24(6):1075–1078. doi: 10.1128/jcm.24.6.1075-1078.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shepherd A. J., Leman P. A., Hummitzsch D. E., Swanepoel R. A comparison of serological techniques for plague surveillance. Trans R Soc Trop Med Hyg. 1984;78(6):771–773. doi: 10.1016/0035-9203(84)90014-2. [DOI] [PubMed] [Google Scholar]
  17. VOGEL F., PETTENKOFER H. J., HELMBOLD W. [On the population genetics of ABO blood groups. 2. Gene frequency and epidemic diseases]. Acta Genet Stat Med. 1960;10:267–294. [PubMed] [Google Scholar]
  18. Williams J. E., Arntzen L., Robinson D. M., Cavanaugh D. C., Isaäcson M. Comparison of passive haemagglutination and enzyme-linked immunosorbent assay for serodiagnosis of plague. Bull World Health Organ. 1982;60(5):777–781. [PMC free article] [PubMed] [Google Scholar]
  19. Williams J. E., Arntzen L., Tyndal G. L., Isaäcson M. Application of enzyme immunoassays for the confirmation of clinically suspect plague in Namibia, 1982. Bull World Health Organ. 1986;64(5):745–752. [PMC free article] [PubMed] [Google Scholar]
  20. Williams J. E., Cavanaugh D. C. Measuring the efficacy of vaccination in affording protection against plague. Bull World Health Organ. 1979;57(2):309–313. [PMC free article] [PubMed] [Google Scholar]

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