Abstract
A latex coagglutination assay was developed to identify rough (R) isolates of Brucella. Latex beads were coated, via protein A, with either an anti-Brucella rough-lipopolysaccharide (R-LPS) monoclonal antibody (MAb) or an anti-Brucella 25-kDa outer membrane protein (Omp25) MAb. Slide agglutination tests were done for 68 strains of Brucella spp., including type strains of all biovars as well as field isolates. Latex beads coated with MAb to R-LPS coagglutinated only R strains, whereas latex beads coated with MAb to Omp25 coagglutinated all the R Brucella isolates except Brucella ovis. Coagglutination was easier to read than agglutination with rabbit R-Brucella-specific antiserum. Thus, this assay accurately differentiates B. ovis from other R Brucella isolates. The latex coagglutination assay can substitute, to advantage, for the current anti-Brucella (R) rabbit monospecific serum.
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Selected References
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- ALTON G. G. The occurrence of dissociated strains of Brucella melitensis in the milk of goats in Malta. J Comp Pathol. 1960 Jan;70:10–17. doi: 10.1016/s0368-1742(60)80002-1. [DOI] [PubMed] [Google Scholar]
- Bowden R. A., Cloeckaert A., Zygmunt M. S., Bernard S., Dubray G. Surface exposure of outer membrane protein and lipopolysaccharide epitopes in Brucella species studied by enzyme-linked immunosorbent assay and flow cytometry. Infect Immun. 1995 Oct;63(10):3945–3952. doi: 10.1128/iai.63.10.3945-3952.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cloeckaert A., Jacques I., Bowden R. A., Dubray G., Limet J. N. Monoclonal antibodies to Brucella rough lipopolysaccharide: characterization and evaluation of their protective effect against B. abortus. Res Microbiol. 1993 Jul-Aug;144(6):475–484. doi: 10.1016/0923-2508(93)90055-7. [DOI] [PubMed] [Google Scholar]
- Cloeckaert A., Verger J. M., Grayon M., Zygmunt M. S., Grépinet O. Nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of Brucella ovis: Evidence for antigenic shift, compared with other Brucella species, due to a deletion in the gene. Infect Immun. 1996 Jun;64(6):2047–2055. doi: 10.1128/iai.64.6.2047-2055.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cloeckaert A., Zygmunt M. S., Bézard G., Dubray G. Purification and antigenic analysis of the major 25-kilodalton outer membrane protein of Brucella abortus. Res Microbiol. 1996 May;147(4):225–235. doi: 10.1016/0923-2508(96)81383-0. [DOI] [PubMed] [Google Scholar]
- Cloeckaert A., de Wergifosse P., Dubray G., Limet J. N. Identification of seven surface-exposed Brucella outer membrane proteins by use of monoclonal antibodies: immunogold labeling for electron microscopy and enzyme-linked immunosorbent assay. Infect Immun. 1990 Dec;58(12):3980–3987. doi: 10.1128/iai.58.12.3980-3987.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Da Costa M., Guillou J. P., Garin-Bastuji B., Thiébaud M., Dubray G. Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification. J Appl Bacteriol. 1996 Sep;81(3):267–275. doi: 10.1111/j.1365-2672.1996.tb04328.x. [DOI] [PubMed] [Google Scholar]
- Roop R. M., 2nd, Preston-Moore D., Bagchi T., Schurig G. G. Rapid identification of smooth Brucella species with a monoclonal antibody. J Clin Microbiol. 1987 Nov;25(11):2090–2093. doi: 10.1128/jcm.25.11.2090-2093.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vizcaíno N., Fernández-Lago L. A rapid and sensitive method for the identification of Brucella species with a monoclonal antibody. Res Microbiol. 1992 Jun;143(5):513–518. doi: 10.1016/0923-2508(92)90098-9. [DOI] [PubMed] [Google Scholar]