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. 1997 Sep;4(5):615–619. doi: 10.1128/cdli.4.5.615-619.1997

Monoclonal antibody E8-18 identifies an integral membrane surface protein unique to Mycoplasma capricolum subsp. capripneumoniae.

F R Rurangirwa 1, P S Shompole 1, A N Wambugu 1, S M Kihara 1, T C McGuire 1
PMCID: PMC170610  PMID: 9302216

Abstract

Monoclonal antibody (MAb) E8-18 reacted with four isolates of Mycoplasma capricolum subsp. capripneumoniae in Western blots identifying an epitope on a 24 kDa antigen (p24). MAb E8-18 did not react with 11 isolates belonging to four other Mycoplasma species or subspecies closely related to M. capricolum subsp. capripneumoniae. A combination of trypsin treatment of intact organisms and detergent-phase partitioning revealed p24 to be an integral M. capricolum subsp. capripneumoniae surface membrane protein.

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Selected References

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