Skip to main content
NCBI home page
Clinical and Diagnostic Laboratory Immunology logoLink to Clinical and Diagnostic Laboratory Immunology
. 1997 Nov;4(6):711–714. doi: 10.1128/cdli.4.6.711-714.1997

Conservation of fimbriae and the hemagglutinating adhesin HA-Ag2 among Porphyromonas gingivalis strains and other anaerobic bacteria studied by epitope mapping analysis.

L Du 1, P Pellen-Mussi 1, F Chandad 1, C Mouton 1, M Bonnaure-Mallet 1
PMCID: PMC170645  PMID: 9384294

Abstract

Monoclonal antibodies characterized as antifimbria and anti-HA-Ag2 were used in immunoblotting to examine the antigenic distribution of fimbriae and HA-Ag2 among a collection of human and animal Porphyromonas strains and human Prevotella and Bacteroides strains. The results showed that fimbrial and HA-Ag2 antigenic structures are peculiar to the species Porphyromonas gingivalis.

Full Text

The Full Text of this article is available as a PDF (690.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ansorge W. Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate. J Biochem Biophys Methods. 1985 May;11(1):13–20. doi: 10.1016/0165-022x(85)90037-5. [DOI] [PubMed] [Google Scholar]
  2. Brook I., Gober A. E. Bacteroides melaninogenicus. Its recovery from tonsils of children with acute tonsillitis. Arch Otolaryngol. 1983 Dec;109(12):818–820. doi: 10.1001/archotol.1983.00800260040010. [DOI] [PubMed] [Google Scholar]
  3. Chandad F., Mouton C. Antigenic, structural, and functional relationships between fimbriae and the hemagglutinating adhesin HA-Ag2 of Porphyromonas gingivalis. Infect Immun. 1995 Dec;63(12):4755–4763. doi: 10.1128/iai.63.12.4755-4763.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chandad F., Mouton C. Molecular size variation of the hemagglutinating adhesin HA-Ag2, a common antigen of Bacteroides gingivalis. Can J Microbiol. 1990 Oct;36(10):690–696. doi: 10.1139/m90-117. [DOI] [PubMed] [Google Scholar]
  5. Deslauriers M., Mouton C. Targetting the production of monoclonal antibodies to the hemagglutinating adhesin of Bacteroides (Porphyromonas) gingivalis by injection of an immunoprecipitate from crossed immunoelectrophoresis. J Biol Buccale. 1991 Jun;19(2):155–160. [PubMed] [Google Scholar]
  6. Dickinson D. P., Kubiniec M. A., Yoshimura F., Genco R. J. Molecular cloning and sequencing of the gene encoding the fimbrial subunit protein of Bacteroides gingivalis. J Bacteriol. 1988 Apr;170(4):1658–1665. doi: 10.1128/jb.170.4.1658-1665.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Du L., Pellen-Mussi P., Chandad F., Mouton C., Bonnaure-Mallet M. Fimbriae and the hemagglutinating adhesin HA-Ag2 mediate adhesion of Porphyromonas gingivalis to epithelial cells. Infect Immun. 1997 Sep;65(9):3875–3881. doi: 10.1128/iai.65.9.3875-3881.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Haapasalo M., Ranta H., Ranta K., Shah H. Black-pigmented Bacteroides spp. in human apical periodontitis. Infect Immun. 1986 Jul;53(1):149–153. doi: 10.1128/iai.53.1.149-153.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hamada N., Sojar H. T., Cho M. I., Genco R. J. Isolation and characterization of a minor fimbria from Porphyromonas gingivalis. Infect Immun. 1996 Nov;64(11):4788–4794. doi: 10.1128/iai.64.11.4788-4794.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hanazawa S., Hirose K., Ohmori Y., Amano S., Kitano S. Bacteroides gingivalis fimbriae stimulate production of thymocyte-activating factor by human gingival fibroblasts. Infect Immun. 1988 Jan;56(1):272–274. doi: 10.1128/iai.56.1.272-274.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Isogai H., Isogai E., Yoshimura F., Suzuki T., Kagota W., Takano K. Specific inhibition of adherence of an oral strain of Bacteroides gingivalis 381 to epithelial cells by monoclonal antibodies against the bacterial fimbriae. Arch Oral Biol. 1988;33(7):479–485. doi: 10.1016/0003-9969(88)90028-3. [DOI] [PubMed] [Google Scholar]
  12. Joe A., Yamamoto A., McBride B. C. Characterization of recombinant and native forms of a cell surface antigen of Porphyromonas (Bacteroides) gingivalis. Infect Immun. 1993 Aug;61(8):3294–3303. doi: 10.1128/iai.61.8.3294-3303.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jones C. H., Jacob-Dubuisson F., Dodson K., Kuehn M., Slonim L., Striker R., Hultgren S. J. Adhesin presentation in bacteria requires molecular chaperones and ushers. Infect Immun. 1992 Nov;60(11):4445–4451. doi: 10.1128/iai.60.11.4445-4451.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kinder S. A., Holt S. C. Characterization of coaggregation between Bacteroides gingivalis T22 and Fusobacterium nucleatum T18. Infect Immun. 1989 Nov;57(11):3425–3433. doi: 10.1128/iai.57.11.3425-3433.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kokeguchi S., Miyamoto M., Ohyama H., Hongyo H., Takigawa M., Kurihara H., Murayama Y., Kato K. Biochemical properties of the major outer membrane proteins of Porphyromonas gingivalis. Microbios. 1994;77(313):247–252. [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Laliberté M., Mayrand D. Characterization of black-pigmented Bacteroides strains isolated from animals. J Appl Bacteriol. 1983 Oct;55(2):247–252. doi: 10.1111/j.1365-2672.1983.tb01322.x. [DOI] [PubMed] [Google Scholar]
  19. Lee J. Y., Sojar H. T., Bedi G. S., Genco R. J. Porphyromonas (Bacteroides) gingivalis fimbrillin: size, amino-terminal sequence, and antigenic heterogeneity. Infect Immun. 1991 Jan;59(1):383–389. doi: 10.1128/iai.59.1.383-389.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Matsushita K., Nagaoka S., Arakaki R., Kawabata Y., Iki K., Kawagoe M., Takada H. Immunobiological activities of a 55-kilodalton cell surface protein of Prevotella intermedia ATCC 25611. Infect Immun. 1994 Jun;62(6):2459–2469. doi: 10.1128/iai.62.6.2459-2469.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ogawa T., Mukai T., Yasuda K., Shimauchi H., Toda Y., Hamada S. Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species. Oral Microbiol Immunol. 1991 Dec;6(6):332–340. doi: 10.1111/j.1399-302x.1991.tb00504.x. [DOI] [PubMed] [Google Scholar]
  22. Ogawa T., Ogo H., Kinoshita A. Antagonistic effect of synthetic peptides corresponding to the binding regions within fimbrial subunit protein from Porphyromonas gingivalis to human gingival fibroblasts. Vaccine. 1997 Feb;15(2):230–236. doi: 10.1016/s0264-410x(96)00127-2. [DOI] [PubMed] [Google Scholar]
  23. Ogawa T., Yasuda K., Yamada K., Mori H., Ochiai K., Hasegawa M. Immunochemical characterisation and epitope mapping of a novel fimbrial protein (Pg-II fimbria) of Porphyromonas gingivalis. FEMS Immunol Med Microbiol. 1995 Jun;11(3):247–255. doi: 10.1111/j.1574-695X.1995.tb00122.x. [DOI] [PubMed] [Google Scholar]
  24. Okuda K., Takazoe I. Haemagglutinating activity of Bacteroides melaninogenicus. Arch Oral Biol. 1974 May;19(5):415–416. doi: 10.1016/0003-9969(74)90184-8. [DOI] [PubMed] [Google Scholar]
  25. Parent R., Mouton C., Lamonde L., Bouchard D. Human and animal serotypes of Bacteroides gingivalis defined by crossed immunoelectrophoresis. Infect Immun. 1986 Mar;51(3):909–918. doi: 10.1128/iai.51.3.909-918.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Slots J., Genco R. J. Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction. J Dent Res. 1984 Mar;63(3):412–421. doi: 10.1177/00220345840630031101. [DOI] [PubMed] [Google Scholar]
  27. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Weinberg A., Belton C. M., Park Y., Lamont R. J. Role of fimbriae in Porphyromonas gingivalis invasion of gingival epithelial cells. Infect Immun. 1997 Jan;65(1):313–316. doi: 10.1128/iai.65.1.313-316.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Woo D. D., Holt S. C., Leadbetter E. R. Ultrastructure of Bacteroides species: Bacteroides asaccharolyticus, Bacteroides fragilis, Bacteroides melaninogenicus subspecies melaninogenicus, and B. melaninogenicus subspecies intermedius. J Infect Dis. 1979 May;139(5):534–546. doi: 10.1093/infdis/139.5.534. [DOI] [PubMed] [Google Scholar]
  30. Yoshimura F., Takahashi K., Nodasaka Y., Suzuki T. Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis. J Bacteriol. 1984 Dec;160(3):949–957. doi: 10.1128/jb.160.3.949-957.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Yoshimura F., Takahashi Y., Hibi E., Takasawa T., Kato H., Dickinson D. P. Proteins with molecular masses of 50 and 80 kilodaltons encoded by genes downstream from the fimbrilin gene (fimA) are components associated with fimbriae in the oral anaerobe Porphyromonas gingivalis. Infect Immun. 1993 Dec;61(12):5181–5189. doi: 10.1128/iai.61.12.5181-5189.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. van Winkelhoff A. J., Carlee A. W., de Graaff J. Bacteroides endodontalis and other black-pigmented Bacteroides species in odontogenic abscesses. Infect Immun. 1985 Sep;49(3):494–497. doi: 10.1128/iai.49.3.494-497.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Diagnostic Laboratory Immunology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES