Abstract
Experiments to determine optimum yields of roquefortine, isofumigaclavine A, and PR toxin, metabolites from Penicillum roqueforti Thom, were performed. Four strains, isolated from blue cheese, and five liquid media were evaluated, although not all permutations were studied. Sucrose (15%)-yeast extract (2%) was the medium chosen for time-course studies at 25 and 15 degrees C using one favorable strain. At 25 degrees C, maximum estimated yields of roquefortine were about 100 mg/liter in the mycelium by 16 days, and no subsequent degradation of this alkaloid was observed. On the other hand, production of PR toxin in the medium peaked at 770 mg/liter at 21 days. At 15 degrees C, yields of roquefortine and PR toxin after 49 days were 60 to 70% of the maximum yields obtained at 25 degrees C. However, about three times more isofumigaclavine A (up to 11 mg/liter) was formed in the mycelium at 15 degrees C than at 25 degrees C. All four strains of P. roqueforti procedure both roquefortine and PR toxin on the sucrose-yeast extract medium at 25 degrees C; isofumigaclavine A was detected in all but one strain grown on this medium.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ARCAMONE F., CHAIN E. B., FERRETTI A., MINGHETTI A., PENNELLA P., TONOLO A. The effect of tryptophan and methyltryptophans on submerged lysergic acid fermentation by Claviceps paspali Stevens and Hall. Biochim Biophys Acta. 1962 Feb 12;57:174–176. doi: 10.1016/0006-3002(62)91103-4. [DOI] [PubMed] [Google Scholar]
- ARCAMONE F., CHAIN E. B., FERRETTI A., MINGHETTI A., PENNELLA P., TONOLO A., VERO L. Production of a new lysergic acid derivative in submerged culture by a strain of Claviceps paspali Stevens & Hall. Proc R Soc Lond B Biol Sci. 1961 Oct 17;155:26–54. doi: 10.1098/rspb.1961.0056. [DOI] [PubMed] [Google Scholar]
- Orth R. PR-Toxinbildung bei Penicillium roqueforti-Stämmen. Z Lebensm Unters Forsch. 1976 Feb 27;160(2):131–136. doi: 10.1007/BF01259958. [DOI] [PubMed] [Google Scholar]
- Rao K. K., Patel V. P., Patel B. Alkaloid production by Aspergillus fumigatus as influenced by changes in substrate composition. Indian J Exp Biol. 1974 Jan;12(1):76–78. [PubMed] [Google Scholar]
- Scott P. M., Kennedy P. C. Analysis of blue cheese for roquefortine and other alkaloids from Penicillium roqueforti. J Agric Food Chem. 1976 Jul-Aug;24(4):865–868. doi: 10.1021/jf60206a028. [DOI] [PubMed] [Google Scholar]
- Scott P. M., Lawrence J. W., van Walbeek W. Detection of mycotoxins by thin-layer chromatography: application to screening of fungal extracts. Appl Microbiol. 1970 Nov;20(5):839–842. doi: 10.1128/am.20.5.839-842.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wei R. D., Still P. E., Smalley E. B., Schnoes H. K., Strong F. M. Isolation and partial characterization of a mycotoxin from Penicillium roqueforti. Appl Microbiol. 1973 Jan;25(1):111–114. doi: 10.1128/am.25.1.111-114.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]