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. 1977 Feb;33(2):257–261. doi: 10.1128/aem.33.2.257-261.1977

Effect of culture conditions on synthesis of L-asparaginase by Escherichia coli A-1.

W R Barnes, G L Dorn, G R Vela
PMCID: PMC170675  PMID: 15509

Abstract

The nutritional requirements and culture conditions affecting biosynthesis of L-asparaginase in a mutant of Escherichia coli HAP designated strain A-1 were studied. Asparaginase activity was increased by the addition of L-glutamic acid, L-glutamine, or commercial-grade monosodium glutamate. The rate of enzyme synthesis was dependent on the interaction between the pH of the culture and the amount of oxygen dissolved in the medium. A critical oxygen transfer rate essential for asparaginase formation was identified, and a fermentation procedure is described in which enzyme synthesis is controlled by aeration rate. Enhancement of L-asparaginase activity by monosodium glutamate was inhibited by the presence of glucose, culture pH, chloramphenicol, and oxygen dissolved in the fermentation medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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