Abstract
Lake Mendota sediments were studied to determine the role of H2 in sediment methanogenesis. H2 was generally not detectable in sediment. The addition of H2 to sediment significantly increased methanogenensis. The amount of methane produced was proportional to the concentration of hydrogen added. H2 addition stimulated the reduction of CO2 to methane, but did not significantly stimulate the conversion of methanol or the methyl position of acetate to methane. Various organic compounds also stimulated sediment methanogenesis. Formate, ethanol, and glucose were shown to serve as electron donors for CO2 reduction to methane. The addition of formate to sediment resulted in H2 evolution. H2 was not deith the phenomenon of interspecies hydrogen transfer. The results indicate that hydrogen is an important intermediate and a rate-limiting factor in sediment methanogenesis.
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Selected References
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- Bell G. R., LeGall L., Peck H. D. Evidence for the periplasmic location of hydrogenase in Desulfovibrio gigas. J Bacteriol. 1974 Nov;120(2):994–997. doi: 10.1128/jb.120.2.994-997.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bryant M. P., Wolin E. A., Wolin M. J., Wolfe R. S. Methanobacillus omelianskii, a symbiotic association of two species of bacteria. Arch Mikrobiol. 1967;59(1):20–31. doi: 10.1007/BF00406313. [DOI] [PubMed] [Google Scholar]
- Cappenberg T. E. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. I. Field observations. Antonie Van Leeuwenhoek. 1974;40(2):285–295. doi: 10.1007/BF00394387. [DOI] [PubMed] [Google Scholar]
- Cappenberg T. E. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. II. Inhibition experiments. Antonie Van Leeuwenhoek. 1974;40(2):297–306. doi: 10.1007/BF00394388. [DOI] [PubMed] [Google Scholar]
- Cappenberg T. E., Prins R. A. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. 3. Experiments with 14C-labeled substrates. Antonie Van Leeuwenhoek. 1974;40(3):457–469. doi: 10.1007/BF00399358. [DOI] [PubMed] [Google Scholar]
- Hungate R. E. Hydrogen as an intermediate in the rumen fermentation. Arch Mikrobiol. 1967;59(1):158–164. doi: 10.1007/BF00406327. [DOI] [PubMed] [Google Scholar]
- Nelson D. R., Zeikus J. G. Rapid method for the radioisotopic analysis of gaseous end products of anaerobic metabolism. Appl Microbiol. 1974 Aug;28(2):258–261. doi: 10.1128/am.28.2.258-261.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oremland R. S. Methane production in shallow-water, tropical marine sediments. Appl Microbiol. 1975 Oct;30(4):602–608. doi: 10.1128/am.30.4.602-608.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scheifinger C. C., Linehan B., Wolin M. J. H2 production by Selenomonas ruminantium in the absence and presence of methanogenic bacteria. Appl Microbiol. 1975 Apr;29(4):480–483. doi: 10.1128/am.29.4.480-483.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith P. H., Mah R. A. Kinetics of acetate metabolism during sludge digestion. Appl Microbiol. 1966 May;14(3):368–371. doi: 10.1128/am.14.3.368-371.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolfe R. S. Microbial formation of methane. Adv Microb Physiol. 1971;6:107–146. doi: 10.1016/s0065-2911(08)60068-5. [DOI] [PubMed] [Google Scholar]
- Wolin M. J. Metabolic interactions among intestinal microorganisms. Am J Clin Nutr. 1974 Nov;27(11):1320–1328. doi: 10.1093/ajcn/27.11.1320. [DOI] [PubMed] [Google Scholar]
- Zeikus J. G., Winfrey M. R. Temperature limitation of methanogenesis in aquatic sediments. Appl Environ Microbiol. 1976 Jan;31(1):99–107. doi: 10.1128/aem.31.1.99-107.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]