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. 1977 Feb;33(2):400–405. doi: 10.1128/aem.33.2.400-405.1977

Isolation and molecular size of Clostridium botulinum type C toxin.

B Syuto, S Kubo
PMCID: PMC170697  PMID: 403861

Abstract

A procedure is described for the purification of hemagglutinin-free Clostridium botulinum type C toxin. The toxin was purified approximately 1,000-fold from the original culture supernatant in an overall yield of 60% to a final specific toxicity of 4.4 x 10(7) minimal lethal doses/mg of protein. The toxin had a molecular weight of 141,000 and consisted of a heavy and a light chain. The molecular weights of the subunits were approximately 98,000 and 53,000. When comparing the molecular size and composition of type C toxin to that of botulinum toxins of different types, some common features may be suggested; i.e., the toxin has a molecular weight between 141,000 to 160,000 and is comprised of a heavy and a light chain linked by disulfide bonds (or bond).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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