Abstract
Hydrogen sulfide-producing strains of salmonellae, Escherichia coli, Citrobacter freundii, and Proteus mirabilis were isolated from fresh pork sausage. All the strains produced black-centered colonies on Hektoen enteric agar (HE). On xylose lysine deoxycholate agar (XLD), C. freundii produced yellow colonies, and the strains of the other three genera formed black-centered colonies. The selectivity of HE and XLD for salmonellae was improved by the addition of novobiocin to both media. With increasing concentrations of novobiocin, the degree of growth inhibition for the four genera was less on HE than on XLD. Novobiocin concentrations of 80 μg/ml in HE and 5 μg/ml in XLD did not affect the growth or colonial morphology of salmonellae. When 80 μg of novobiocin per ml was incorporated into HE, P. mirabilis strains were not recovered, 40% of C. freundii strains failed to form black-centered colonies, and growth of E. coli strains was not affected but colonies were altered without eliminating the black centers. When novobiocin at 5 μg/ml was incorporated into XLD, colonies of P. mirabilis strains were not recovered, C. freundii formed yellow colonies, and the colonies of the H2S-producing E. coli strains were unaffected.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bisciello N. B., Jr, Schrade J. P. Evaluation of hektoen enteric agar for the detection of Salmonella in foods and feeds. J Assoc Off Anal Chem. 1974 Jul;57(4):992–996. [PubMed] [Google Scholar]
- Boothroyd M., Baird-Parker A. C. The use of enrichment serology for Salmonella detection in human foods and animal feeds. J Appl Bacteriol. 1973 Mar;36(1):165–172. doi: 10.1111/j.1365-2672.1973.tb04084.x. [DOI] [PubMed] [Google Scholar]
- Darland G., Davis B. R. Biochemical and serological characterization of hydrogen sulfide-positive variants of Escherichia coli. Appl Microbiol. 1974 Jan;27(1):54–58. doi: 10.1128/am.27.1.54-58.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoben D. A., Ashton D. H., Peterson A. C. A rapid, presumptive procedure for the detection of Salmonella in foods and food ingredients. Appl Microbiol. 1973 Jan;25(1):123–129. doi: 10.1128/am.25.1.123-129.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoben D. A., Ashton D. H., Peterson A. C. Some observations on the incorporation of novobiocin into Hektoen enteric agar for improved Salmonella isolation. Appl Microbiol. 1973 Jul;26(1):126–127. doi: 10.1128/am.26.1.126-127.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JEFFRIES L. Novobiocin-tetrathionate broth: a medium of improved selectivity for the isolation of Salmonellae from faeces. J Clin Pathol. 1959 Nov;12:568–571. doi: 10.1136/jcp.12.6.568. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moats W. A., Kinner J. A. Observations on brilliant green agar with H2S indicator. Appl Environ Microbiol. 1976 Mar;31(3):380–384. doi: 10.1128/aem.31.3.380-384.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shanson D. C. A new selective medium for the isolation of Salmonellae other than Salmonella typhi. J Med Microbiol. 1975 May;8(2):357–364. doi: 10.1099/00222615-8-2-357. [DOI] [PubMed] [Google Scholar]
- Smith P. B., Tomfohrde K. M., Rhoden D. L., Balows A. API system: a multitube micromethod for identification of Enterobacteriaceae. Appl Microbiol. 1972 Sep;24(3):449–452. doi: 10.1128/am.24.3.449-452.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor W. I., Schelhart D. Isolation of Shigellae. 8. Comparison of xylose lysine deoxycholate agar, hektoen enteric agar, Salmonella-Shigella agar, and eosin methylene blue agar with stool specimens. Appl Microbiol. 1971 Jan;21(1):32–37. doi: 10.1128/am.21.1.32-37.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Dijck P., van de Voorde H. Sensitivity of environmental microorganisms to antimicrobial agents. Appl Environ Microbiol. 1976 Mar;31(3):332–336. doi: 10.1128/aem.31.3.332-336.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]